Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots

Cyst nematodes are important herbivorous pests in agriculture that obtain nutrients through specialized root structures termed syncytia. Syncytium initiation, development, and functioning are a research focus because syncytia are the primary interface for molecular interactions between the host plan...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marcin Filipecki, Marek Żurczak, Mateusz Matuszkiewicz, Magdalena Święcicka, Wojciech Kurek, Jarosław Olszewski, Marek Daniel Koter, Douglas Lamont, Mirosław Sobczak
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Globodera rostochiensis</i> <i>Solanum lycopersicum</i> syncytium proteome laser capture microdissection mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/af4a019eee4b420b8d527bc13632c1ea
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:af4a019eee4b420b8d527bc13632c1ea
record_format	dspace
spelling	oai:doaj.org-article:af4a019eee4b420b8d527bc13632c1ea2021-11-25T17:53:48ZProfiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots10.3390/ijms2222121471422-00671661-6596https://doaj.org/article/af4a019eee4b420b8d527bc13632c1ea2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12147https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cyst nematodes are important herbivorous pests in agriculture that obtain nutrients through specialized root structures termed syncytia. Syncytium initiation, development, and functioning are a research focus because syncytia are the primary interface for molecular interactions between the host plant and parasite. The small size and complex development (over approximately two weeks) of syncytia hinder precise analyses, therefore most studies have analyzed the transcriptome of infested whole-root systems or syncytia-containing root segments. Here, we describe an effective procedure to microdissect syncytia induced by <i>Globodera rostochiensis</i> from tomato roots and to analyze the syncytial proteome using mass spectrometry. As little as 15 mm<sup>2</sup> of 10-µm-thick sections dissected from 30 syncytia enabled the identification of 100–200 proteins in each sample, indicating that mass-spectrometric methods currently in use achieved acceptable sensitivity for proteome profiling of microscopic samples of plant tissues (approximately 100 µg). Among the identified proteins, 48 were specifically detected in syncytia and 7 in uninfected roots. The occurrence of approximately 50% of these proteins in syncytia was not correlated with transcript abundance estimated by quantitative reverse-transcription PCR analysis. The functional categories of these proteins confirmed that protein turnover, stress responses, and intracellular trafficking are important components of the proteome dynamics of developing syncytia.Marcin FilipeckiMarek ŻurczakMateusz MatuszkiewiczMagdalena ŚwięcickaWojciech KurekJarosław OlszewskiMarek Daniel KoterDouglas LamontMirosław SobczakMDPI AGarticle<i>Globodera rostochiensis</i><i>Solanum lycopersicum</i>syncytiumproteomelaser capture microdissectionmass spectrometryBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12147, p 12147 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Globodera rostochiensis</i> <i>Solanum lycopersicum</i> syncytium proteome laser capture microdissection mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Globodera rostochiensis</i> <i>Solanum lycopersicum</i> syncytium proteome laser capture microdissection mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999 Marcin Filipecki Marek Żurczak Mateusz Matuszkiewicz Magdalena Święcicka Wojciech Kurek Jarosław Olszewski Marek Daniel Koter Douglas Lamont Mirosław Sobczak Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots
description	Cyst nematodes are important herbivorous pests in agriculture that obtain nutrients through specialized root structures termed syncytia. Syncytium initiation, development, and functioning are a research focus because syncytia are the primary interface for molecular interactions between the host plant and parasite. The small size and complex development (over approximately two weeks) of syncytia hinder precise analyses, therefore most studies have analyzed the transcriptome of infested whole-root systems or syncytia-containing root segments. Here, we describe an effective procedure to microdissect syncytia induced by <i>Globodera rostochiensis</i> from tomato roots and to analyze the syncytial proteome using mass spectrometry. As little as 15 mm<sup>2</sup> of 10-µm-thick sections dissected from 30 syncytia enabled the identification of 100–200 proteins in each sample, indicating that mass-spectrometric methods currently in use achieved acceptable sensitivity for proteome profiling of microscopic samples of plant tissues (approximately 100 µg). Among the identified proteins, 48 were specifically detected in syncytia and 7 in uninfected roots. The occurrence of approximately 50% of these proteins in syncytia was not correlated with transcript abundance estimated by quantitative reverse-transcription PCR analysis. The functional categories of these proteins confirmed that protein turnover, stress responses, and intracellular trafficking are important components of the proteome dynamics of developing syncytia.
format	article
author	Marcin Filipecki Marek Żurczak Mateusz Matuszkiewicz Magdalena Święcicka Wojciech Kurek Jarosław Olszewski Marek Daniel Koter Douglas Lamont Mirosław Sobczak
author_facet	Marcin Filipecki Marek Żurczak Mateusz Matuszkiewicz Magdalena Święcicka Wojciech Kurek Jarosław Olszewski Marek Daniel Koter Douglas Lamont Mirosław Sobczak
author_sort	Marcin Filipecki
title	Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots
title_short	Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots
title_full	Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots
title_fullStr	Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots
title_full_unstemmed	Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots
title_sort	profiling the proteome of cyst nematode-induced syncytia on tomato roots
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/af4a019eee4b420b8d527bc13632c1ea
work_keys_str_mv	AT marcinfilipecki profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots AT marekzurczak profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots AT mateuszmatuszkiewicz profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots AT magdalenaswiecicka profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots AT wojciechkurek profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots AT jarosławolszewski profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots AT marekdanielkoter profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots AT douglaslamont profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots AT mirosławsobczak profilingtheproteomeofcystnematodeinducedsyncytiaontomatoroots
_version_	1718411880660205568

Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots

Ejemplares similares