The type 3 secretion system requires actin polymerization to open translocon pores.

Many bacterial pathogens require a type 3 secretion system (T3SS) to establish a niche. Host contact activates bacterial T3SS assembly of a translocon pore in the host plasma membrane. Following pore formation, the T3SS docks onto the translocon pore. Docking establishes a continuous passage that en...

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Autores principales:	Brian C Russo, Jeffrey K Duncan-Lowey, Poyin Chen, Marcia B Goldberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/af4cef629e8b44c59266d04ecf09c18c
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spelling	oai:doaj.org-article:af4cef629e8b44c59266d04ecf09c18c2021-12-02T20:00:12ZThe type 3 secretion system requires actin polymerization to open translocon pores.1553-73661553-737410.1371/journal.ppat.1009932https://doaj.org/article/af4cef629e8b44c59266d04ecf09c18c2021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009932https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Many bacterial pathogens require a type 3 secretion system (T3SS) to establish a niche. Host contact activates bacterial T3SS assembly of a translocon pore in the host plasma membrane. Following pore formation, the T3SS docks onto the translocon pore. Docking establishes a continuous passage that enables the translocation of virulence proteins, effectors, into the host cytosol. Here we investigate the contribution of actin polymerization to T3SS-mediated translocation. Using the T3SS model organism Shigella flexneri, we show that actin polymerization is required for assembling the translocon pore in an open conformation, thereby enabling effector translocation. Opening of the pore channel is associated with a conformational change to the pore, which is dependent upon actin polymerization and a coiled-coil domain in the pore protein IpaC. Analysis of an IpaC mutant that is defective in ruffle formation shows that actin polymerization-dependent pore opening is distinct from the previously described actin polymerization-dependent ruffles that are required for bacterial internalization. Moreover, actin polymerization is not required for other pore functions, including docking or pore protein insertion into the plasma membrane. Thus, activation of the T3SS is a multilayered process in which host signals are sensed by the translocon pore leading to the activation of effector translocation.Brian C RussoJeffrey K Duncan-LoweyPoyin ChenMarcia B GoldbergPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 9, p e1009932 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Brian C Russo Jeffrey K Duncan-Lowey Poyin Chen Marcia B Goldberg The type 3 secretion system requires actin polymerization to open translocon pores.
description	Many bacterial pathogens require a type 3 secretion system (T3SS) to establish a niche. Host contact activates bacterial T3SS assembly of a translocon pore in the host plasma membrane. Following pore formation, the T3SS docks onto the translocon pore. Docking establishes a continuous passage that enables the translocation of virulence proteins, effectors, into the host cytosol. Here we investigate the contribution of actin polymerization to T3SS-mediated translocation. Using the T3SS model organism Shigella flexneri, we show that actin polymerization is required for assembling the translocon pore in an open conformation, thereby enabling effector translocation. Opening of the pore channel is associated with a conformational change to the pore, which is dependent upon actin polymerization and a coiled-coil domain in the pore protein IpaC. Analysis of an IpaC mutant that is defective in ruffle formation shows that actin polymerization-dependent pore opening is distinct from the previously described actin polymerization-dependent ruffles that are required for bacterial internalization. Moreover, actin polymerization is not required for other pore functions, including docking or pore protein insertion into the plasma membrane. Thus, activation of the T3SS is a multilayered process in which host signals are sensed by the translocon pore leading to the activation of effector translocation.
format	article
author	Brian C Russo Jeffrey K Duncan-Lowey Poyin Chen Marcia B Goldberg
author_facet	Brian C Russo Jeffrey K Duncan-Lowey Poyin Chen Marcia B Goldberg
author_sort	Brian C Russo
title	The type 3 secretion system requires actin polymerization to open translocon pores.
title_short	The type 3 secretion system requires actin polymerization to open translocon pores.
title_full	The type 3 secretion system requires actin polymerization to open translocon pores.
title_fullStr	The type 3 secretion system requires actin polymerization to open translocon pores.
title_full_unstemmed	The type 3 secretion system requires actin polymerization to open translocon pores.
title_sort	type 3 secretion system requires actin polymerization to open translocon pores.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/af4cef629e8b44c59266d04ecf09c18c
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The type 3 secretion system requires actin polymerization to open translocon pores.

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