The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.

The IAPE (Intracisternal A-type Particles elements with an Envelope) family of murine endogenous retroelements is present at more than 200 copies in the mouse genome. We had previously identified a single copy that proved to be fully functional, i.e. which can generate viral particles budding out of...

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Autores principales:	Marie Dewannieux, Cécile Vernochet, David Ribet, Birke Bartosch, François-Loïc Cosset, Thierry Heidmann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/af4d03d099e0422d8ac30e78ac3f4399
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spelling	oai:doaj.org-article:af4d03d099e0422d8ac30e78ac3f43992021-11-18T06:05:14ZThe mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.1553-73661553-737410.1371/journal.ppat.1002309https://doaj.org/article/af4d03d099e0422d8ac30e78ac3f43992011-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22028653/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The IAPE (Intracisternal A-type Particles elements with an Envelope) family of murine endogenous retroelements is present at more than 200 copies in the mouse genome. We had previously identified a single copy that proved to be fully functional, i.e. which can generate viral particles budding out of the cell and infectious on a series of cells, including human cells. We also showed that IAPE are the progenitors of the highly reiterated IAP elements. The latter are now strictly intracellular retrotransposons, due to the loss of the envelope gene and re-localisation of the associated particles in the course of evolution. In the present study we searched for the cellular receptor of the IAPE elements, by using a lentiviral human cDNA library and a pseudotype assay on transduced cells. We identified Ephrin A4, a GPI-anchored molecule involved in several developmental processes, as a receptor for the IAPE pseudotypes. We also found that the other 4 members of the Ephrin A family -but not those of the closely related Ephrin B family- were also able to mediate IAPE cell entry, thus significantly increasing the amount of possible cell types susceptible to IAPE infection. We show that these include mouse germline cells, as illustrated by immunohistochemistry experiments, consistent with IAPE genomic amplification by successive re-infection. We propose that the uncovered properties of the identified receptors played a role in the accumulation of IAPE elements in the mouse genome, and in the survival of a functional copy.Marie DewannieuxCécile VernochetDavid RibetBirke BartoschFrançois-Loïc CossetThierry HeidmannPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 10, p e1002309 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Marie Dewannieux Cécile Vernochet David Ribet Birke Bartosch François-Loïc Cosset Thierry Heidmann The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
description	The IAPE (Intracisternal A-type Particles elements with an Envelope) family of murine endogenous retroelements is present at more than 200 copies in the mouse genome. We had previously identified a single copy that proved to be fully functional, i.e. which can generate viral particles budding out of the cell and infectious on a series of cells, including human cells. We also showed that IAPE are the progenitors of the highly reiterated IAP elements. The latter are now strictly intracellular retrotransposons, due to the loss of the envelope gene and re-localisation of the associated particles in the course of evolution. In the present study we searched for the cellular receptor of the IAPE elements, by using a lentiviral human cDNA library and a pseudotype assay on transduced cells. We identified Ephrin A4, a GPI-anchored molecule involved in several developmental processes, as a receptor for the IAPE pseudotypes. We also found that the other 4 members of the Ephrin A family -but not those of the closely related Ephrin B family- were also able to mediate IAPE cell entry, thus significantly increasing the amount of possible cell types susceptible to IAPE infection. We show that these include mouse germline cells, as illustrated by immunohistochemistry experiments, consistent with IAPE genomic amplification by successive re-infection. We propose that the uncovered properties of the identified receptors played a role in the accumulation of IAPE elements in the mouse genome, and in the survival of a functional copy.
format	article
author	Marie Dewannieux Cécile Vernochet David Ribet Birke Bartosch François-Loïc Cosset Thierry Heidmann
author_facet	Marie Dewannieux Cécile Vernochet David Ribet Birke Bartosch François-Loïc Cosset Thierry Heidmann
author_sort	Marie Dewannieux
title	The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
title_short	The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
title_full	The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
title_fullStr	The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
title_full_unstemmed	The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
title_sort	mouse iape endogenous retrovirus can infect cells through any of the five gpi-anchored ephrin a proteins.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/af4d03d099e0422d8ac30e78ac3f4399
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The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.

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