Interaction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila

Abstract The inverse dosage effect caused by chromosome number variations shows global consequences in genomic imbalance including sexual dimorphism and an X chromosome-specific response. To investigate the relationship of the MSL complex to genomic imbalance, we over-expressed MSL2 in autosomal and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shuai Zhang, Haizhu Qi, Cheng Huang, Lijia Yuan, Ludan Zhang, Ruixue Wang, Yu Tian, Lin Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af50f0dc00f24d648cf3519bd41461c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:af50f0dc00f24d648cf3519bd41461c3
record_format	dspace
spelling	oai:doaj.org-article:af50f0dc00f24d648cf3519bd41461c32021-12-02T18:01:40ZInteraction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila10.1038/s41598-021-99268-y2045-2322https://doaj.org/article/af50f0dc00f24d648cf3519bd41461c32021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99268-yhttps://doaj.org/toc/2045-2322Abstract The inverse dosage effect caused by chromosome number variations shows global consequences in genomic imbalance including sexual dimorphism and an X chromosome-specific response. To investigate the relationship of the MSL complex to genomic imbalance, we over-expressed MSL2 in autosomal and sex chromosomal aneuploids, and analyzed the different transcriptomes. Some candidate genes involved in regulatory mechanisms have also been tested during embryogenesis using TSA-FISH. Here we show that the de novo MSL complex assembled on the X chromosomes in females further reduced the global expression level on the basis of 2/3 down-regulation caused by the inverse dosage effect in trisomy through epigenetic modulations rather than induced dosage compensation. Plus, the sexual dimorphism effect in unbalanced genomes was further examined due to the pre-existing of the MSL complex in males. All these results demonstrate the dynamic functions of the MSL complex on global gene expression in different aneuploid genomes.Shuai ZhangHaizhu QiCheng HuangLijia YuanLudan ZhangRuixue WangYu TianLin SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuai Zhang Haizhu Qi Cheng Huang Lijia Yuan Ludan Zhang Ruixue Wang Yu Tian Lin Sun Interaction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila
description	Abstract The inverse dosage effect caused by chromosome number variations shows global consequences in genomic imbalance including sexual dimorphism and an X chromosome-specific response. To investigate the relationship of the MSL complex to genomic imbalance, we over-expressed MSL2 in autosomal and sex chromosomal aneuploids, and analyzed the different transcriptomes. Some candidate genes involved in regulatory mechanisms have also been tested during embryogenesis using TSA-FISH. Here we show that the de novo MSL complex assembled on the X chromosomes in females further reduced the global expression level on the basis of 2/3 down-regulation caused by the inverse dosage effect in trisomy through epigenetic modulations rather than induced dosage compensation. Plus, the sexual dimorphism effect in unbalanced genomes was further examined due to the pre-existing of the MSL complex in males. All these results demonstrate the dynamic functions of the MSL complex on global gene expression in different aneuploid genomes.
format	article
author	Shuai Zhang Haizhu Qi Cheng Huang Lijia Yuan Ludan Zhang Ruixue Wang Yu Tian Lin Sun
author_facet	Shuai Zhang Haizhu Qi Cheng Huang Lijia Yuan Ludan Zhang Ruixue Wang Yu Tian Lin Sun
author_sort	Shuai Zhang
title	Interaction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila
title_short	Interaction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila
title_full	Interaction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila
title_fullStr	Interaction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila
title_full_unstemmed	Interaction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila
title_sort	interaction of male specific lethal complex and genomic imbalance on global gene expression in drosophila
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/af50f0dc00f24d648cf3519bd41461c3
work_keys_str_mv	AT shuaizhang interactionofmalespecificlethalcomplexandgenomicimbalanceonglobalgeneexpressionindrosophila AT haizhuqi interactionofmalespecificlethalcomplexandgenomicimbalanceonglobalgeneexpressionindrosophila AT chenghuang interactionofmalespecificlethalcomplexandgenomicimbalanceonglobalgeneexpressionindrosophila AT lijiayuan interactionofmalespecificlethalcomplexandgenomicimbalanceonglobalgeneexpressionindrosophila AT ludanzhang interactionofmalespecificlethalcomplexandgenomicimbalanceonglobalgeneexpressionindrosophila AT ruixuewang interactionofmalespecificlethalcomplexandgenomicimbalanceonglobalgeneexpressionindrosophila AT yutian interactionofmalespecificlethalcomplexandgenomicimbalanceonglobalgeneexpressionindrosophila AT linsun interactionofmalespecificlethalcomplexandgenomicimbalanceonglobalgeneexpressionindrosophila
_version_	1718378948406018048

Interaction of Male Specific Lethal complex and genomic imbalance on global gene expression in Drosophila

Ejemplares similares