Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS120

ABSTRACT Prochlorococcus requires the capability to accommodate to environmental changes in order to proliferate in oligotrophic oceans, in particular regarding nitrogen availability. A precise knowledge of the composition and changes in the proteome can yield fundamental insights into such a respon...

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Autores principales:	Maria Agustina Domínguez-Martín, Guadalupe Gómez-Baena, Jesús Díez, Maria José López-Grueso, Robert J. Beynon, José Manuel García-Fernández
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	marine cyanobacteria nitrogen limitation nitrogen metabolism prochlorococcus quantitative proteomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/af529d53517f45b1b679d5c7e14c16c6
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spelling	oai:doaj.org-article:af529d53517f45b1b679d5c7e14c16c62021-12-02T18:39:33ZQuantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS12010.1128/mSystems.00008-172379-5077https://doaj.org/article/af529d53517f45b1b679d5c7e14c16c62017-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00008-17https://doaj.org/toc/2379-5077ABSTRACT Prochlorococcus requires the capability to accommodate to environmental changes in order to proliferate in oligotrophic oceans, in particular regarding nitrogen availability. A precise knowledge of the composition and changes in the proteome can yield fundamental insights into such a response. Here we report a detailed proteome analysis of the important model cyanobacterium Prochlorococcus marinus SS120 after treatment with azaserine, an inhibitor of ferredoxin-dependent glutamate synthase (GOGAT), to simulate extreme nitrogen starvation. In total, 1,072 proteins, corresponding to 57% of the theoretical proteome, were identified—the maximum proteome coverage obtained for any Prochlorococcus strain thus far. Spectral intensity, calibrated quantification by the Hi3 method, was obtained for 1,007 proteins. Statistically significant changes (P value of <0.05) were observed for 408 proteins, with the majority of proteins (92.4%) downregulated after 8 h of treatment. There was a strong decrease in ribosomal proteins upon azaserine addition, while many transporters were increased. The regulatory proteins PII and PipX were decreased, and the global nitrogen regulator NtcA was upregulated. Furthermore, our data for Prochlorococcus indicate that NtcA also participates in the regulation of photosynthesis. Prochlorococcus responds to the lack of nitrogen by slowing down translation, while inducing photosynthetic cyclic electron flow and biosynthesis of proteins involved in nitrogen uptake and assimilation. IMPORTANCE Prochlorococcus is the most abundant photosynthetic organism on Earth, contributing significantly to global primary production and playing a prominent role in biogeochemical cycles. Here we study the effects of extreme nitrogen limitation, a feature of the oligotrophic oceans inhabited by this organism. Quantitative proteomics allowed an accurate quantification of the Prochlorococcus proteome, finding three main responses to nitrogen limitation: upregulation of nitrogen assimilation-related proteins, including transporters; downregulation of ribosome proteins; and induction of the photosystem II cyclic electron flow. This suggests that nitrogen limitation affects a range of metabolic processes far wider than initially believed, with the ultimate goal of saving nitrogen and maximizing the nitrogen uptake and assimilation capabilities of the cell. Author Video: An author video summary of this article is available.Maria Agustina Domínguez-MartínGuadalupe Gómez-BaenaJesús DíezMaria José López-GruesoRobert J. BeynonJosé Manuel García-FernándezAmerican Society for Microbiologyarticlemarine cyanobacterianitrogen limitationnitrogen metabolismprochlorococcusquantitative proteomicsMicrobiologyQR1-502ENmSystems, Vol 2, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	marine cyanobacteria nitrogen limitation nitrogen metabolism prochlorococcus quantitative proteomics Microbiology QR1-502
spellingShingle	marine cyanobacteria nitrogen limitation nitrogen metabolism prochlorococcus quantitative proteomics Microbiology QR1-502 Maria Agustina Domínguez-Martín Guadalupe Gómez-Baena Jesús Díez Maria José López-Grueso Robert J. Beynon José Manuel García-Fernández Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS120
description	ABSTRACT Prochlorococcus requires the capability to accommodate to environmental changes in order to proliferate in oligotrophic oceans, in particular regarding nitrogen availability. A precise knowledge of the composition and changes in the proteome can yield fundamental insights into such a response. Here we report a detailed proteome analysis of the important model cyanobacterium Prochlorococcus marinus SS120 after treatment with azaserine, an inhibitor of ferredoxin-dependent glutamate synthase (GOGAT), to simulate extreme nitrogen starvation. In total, 1,072 proteins, corresponding to 57% of the theoretical proteome, were identified—the maximum proteome coverage obtained for any Prochlorococcus strain thus far. Spectral intensity, calibrated quantification by the Hi3 method, was obtained for 1,007 proteins. Statistically significant changes (P value of <0.05) were observed for 408 proteins, with the majority of proteins (92.4%) downregulated after 8 h of treatment. There was a strong decrease in ribosomal proteins upon azaserine addition, while many transporters were increased. The regulatory proteins PII and PipX were decreased, and the global nitrogen regulator NtcA was upregulated. Furthermore, our data for Prochlorococcus indicate that NtcA also participates in the regulation of photosynthesis. Prochlorococcus responds to the lack of nitrogen by slowing down translation, while inducing photosynthetic cyclic electron flow and biosynthesis of proteins involved in nitrogen uptake and assimilation. IMPORTANCE Prochlorococcus is the most abundant photosynthetic organism on Earth, contributing significantly to global primary production and playing a prominent role in biogeochemical cycles. Here we study the effects of extreme nitrogen limitation, a feature of the oligotrophic oceans inhabited by this organism. Quantitative proteomics allowed an accurate quantification of the Prochlorococcus proteome, finding three main responses to nitrogen limitation: upregulation of nitrogen assimilation-related proteins, including transporters; downregulation of ribosome proteins; and induction of the photosystem II cyclic electron flow. This suggests that nitrogen limitation affects a range of metabolic processes far wider than initially believed, with the ultimate goal of saving nitrogen and maximizing the nitrogen uptake and assimilation capabilities of the cell. Author Video: An author video summary of this article is available.
format	article
author	Maria Agustina Domínguez-Martín Guadalupe Gómez-Baena Jesús Díez Maria José López-Grueso Robert J. Beynon José Manuel García-Fernández
author_facet	Maria Agustina Domínguez-Martín Guadalupe Gómez-Baena Jesús Díez Maria José López-Grueso Robert J. Beynon José Manuel García-Fernández
author_sort	Maria Agustina Domínguez-Martín
title	Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS120
title_short	Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS120
title_full	Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS120
title_fullStr	Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS120
title_full_unstemmed	Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS120
title_sort	quantitative proteomics shows extensive remodeling induced by nitrogen limitation in <named-content content-type="genus-species">prochlorococcus marinus</named-content> ss120
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/af529d53517f45b1b679d5c7e14c16c6
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Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in <named-content content-type="genus-species">Prochlorococcus marinus</named-content> SS120

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