Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.

Caseous lymphadenitis (CLA) or pseudotuberculosis is a chronic zoonotic bacterial disease caused by Corynebacterium pseudotuberculosis, which affects livestock and humans. This study aimed to describe the pathology, bacteriology and confirm the identity of the pathogen by 16S rRNA gene sequencing in...

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Autores principales:	Abdelnasir Mohammed Adam Terab, Ghada El Derdiri Abdel Wahab, Hassan Zackaria Ali Ishag, Nasereldien Altaib Hussein Khalil, El Tigani Ahmed El Tigani-Asil, Farouk Mohamed Hashem, Abdelmalik Ibrahim Khalafalla, Asma Abdi Mohamed Shah, Salama Suhail Mohammed Al Muhairi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af55eda12d434b61a0606db3ea34388d
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spelling	oai:doaj.org-article:af55eda12d434b61a0606db3ea34388d2021-12-02T20:10:59ZPathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.1932-620310.1371/journal.pone.0252893https://doaj.org/article/af55eda12d434b61a0606db3ea34388d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252893https://doaj.org/toc/1932-6203Caseous lymphadenitis (CLA) or pseudotuberculosis is a chronic zoonotic bacterial disease caused by Corynebacterium pseudotuberculosis, which affects livestock and humans. This study aimed to describe the pathology, bacteriology and confirm the identity of the pathogen by 16S rRNA gene sequencing in Camelus dromedarius. A total of 12 camels with suspected CLA in three regions of Abu Dhabi Emirate (Abu Dhabi, Al Ain and Al Dhafra), United Arab Emirate (UAE) were subjected to clinical and postmortem examinations from January 2015 to December 2020. Clinically, camels were emaciated and showed the presence of external caseous abscesses suggestive of CLA. Postmortem examination showed multiple abscesses of variable sizes with caseous material encapsulated by fibrous tissue in the liver, lungs, muscle, and lymph nodes. Following clinical and postmortem examination, blood, pus and different tissue samples were collected for subsequent analysis. Histopathological examination of all organs stained with Hematoxylin and Eosin (H&E) indicated a central caseo-necrotic core that was admixed with bacterial colonies and infiltration of chronic inflammatory cells, surrounded by a pyogenic membrane, and an outer fibrous connective tissue capsule. Bacterial culture identified the isolates of Corynebacterium pseudotuberculosis biotype ovis strain, and these isolates were shown to be sensitive to all antibiotics tested (penicillin, ampicillin, Co-trimoxazole, enrofloxacin and tetracycline). Moreover, the identity of the isolates was confirmed by partial sequencing of the 16S rRNA gene which showed a 100% identity to Corynebacterium pseudotuberculosis. Phylogenetic analysis based on 16S rRNA gene sequence clearly differentiates Corynebacterium pseudotuberculosis from other species of Corynebacterium. Briefly, this study provided the basic information for infection of Corynebacterium pseudotuberculosis in Camels and will help in controlling of this pathogen in the region.Abdelnasir Mohammed Adam TerabGhada El Derdiri Abdel WahabHassan Zackaria Ali IshagNasereldien Altaib Hussein KhalilEl Tigani Ahmed El Tigani-AsilFarouk Mohamed HashemAbdelmalik Ibrahim KhalafallaAsma Abdi Mohamed ShahSalama Suhail Mohammed Al MuhairiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252893 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abdelnasir Mohammed Adam Terab Ghada El Derdiri Abdel Wahab Hassan Zackaria Ali Ishag Nasereldien Altaib Hussein Khalil El Tigani Ahmed El Tigani-Asil Farouk Mohamed Hashem Abdelmalik Ibrahim Khalafalla Asma Abdi Mohamed Shah Salama Suhail Mohammed Al Muhairi Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.
description	Caseous lymphadenitis (CLA) or pseudotuberculosis is a chronic zoonotic bacterial disease caused by Corynebacterium pseudotuberculosis, which affects livestock and humans. This study aimed to describe the pathology, bacteriology and confirm the identity of the pathogen by 16S rRNA gene sequencing in Camelus dromedarius. A total of 12 camels with suspected CLA in three regions of Abu Dhabi Emirate (Abu Dhabi, Al Ain and Al Dhafra), United Arab Emirate (UAE) were subjected to clinical and postmortem examinations from January 2015 to December 2020. Clinically, camels were emaciated and showed the presence of external caseous abscesses suggestive of CLA. Postmortem examination showed multiple abscesses of variable sizes with caseous material encapsulated by fibrous tissue in the liver, lungs, muscle, and lymph nodes. Following clinical and postmortem examination, blood, pus and different tissue samples were collected for subsequent analysis. Histopathological examination of all organs stained with Hematoxylin and Eosin (H&E) indicated a central caseo-necrotic core that was admixed with bacterial colonies and infiltration of chronic inflammatory cells, surrounded by a pyogenic membrane, and an outer fibrous connective tissue capsule. Bacterial culture identified the isolates of Corynebacterium pseudotuberculosis biotype ovis strain, and these isolates were shown to be sensitive to all antibiotics tested (penicillin, ampicillin, Co-trimoxazole, enrofloxacin and tetracycline). Moreover, the identity of the isolates was confirmed by partial sequencing of the 16S rRNA gene which showed a 100% identity to Corynebacterium pseudotuberculosis. Phylogenetic analysis based on 16S rRNA gene sequence clearly differentiates Corynebacterium pseudotuberculosis from other species of Corynebacterium. Briefly, this study provided the basic information for infection of Corynebacterium pseudotuberculosis in Camels and will help in controlling of this pathogen in the region.
format	article
author	Abdelnasir Mohammed Adam Terab Ghada El Derdiri Abdel Wahab Hassan Zackaria Ali Ishag Nasereldien Altaib Hussein Khalil El Tigani Ahmed El Tigani-Asil Farouk Mohamed Hashem Abdelmalik Ibrahim Khalafalla Asma Abdi Mohamed Shah Salama Suhail Mohammed Al Muhairi
author_facet	Abdelnasir Mohammed Adam Terab Ghada El Derdiri Abdel Wahab Hassan Zackaria Ali Ishag Nasereldien Altaib Hussein Khalil El Tigani Ahmed El Tigani-Asil Farouk Mohamed Hashem Abdelmalik Ibrahim Khalafalla Asma Abdi Mohamed Shah Salama Suhail Mohammed Al Muhairi
author_sort	Abdelnasir Mohammed Adam Terab
title	Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.
title_short	Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.
title_full	Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.
title_fullStr	Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.
title_full_unstemmed	Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.
title_sort	pathology, bacteriology and molecular studies on caseous lymphadenitis in camelus dromedarius in the emirate of abu dhabi, uae, 2015-2020.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/af55eda12d434b61a0606db3ea34388d
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Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020.

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