Low proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection

Abstract Follicular regulatory T (Tfr) cell can effectively regulate humoral immunity, but its function and mechanism in antibody-mediated rejection (AMR) after organ transplantation remains unclear. Here we detected follicular helper T (Tfh) cell subsets in 88 renal transplant patients with chronic...

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Autores principales:	Wen Chen, Jian Bai, Haiyan Huang, Lili Bi, Xiangrui Kong, Yu Gao, Yong Han, Li Xiao, Bingyi Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af570329ae904844b525a77d7f204a90
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spelling	oai:doaj.org-article:af570329ae904844b525a77d7f204a902021-12-02T16:05:58ZLow proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection10.1038/s41598-017-01625-32045-2322https://doaj.org/article/af570329ae904844b525a77d7f204a902017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01625-3https://doaj.org/toc/2045-2322Abstract Follicular regulatory T (Tfr) cell can effectively regulate humoral immunity, but its function and mechanism in antibody-mediated rejection (AMR) after organ transplantation remains unclear. Here we detected follicular helper T (Tfh) cell subsets in 88 renal transplant patients with chronic renal allograft dysfunction (40 with AMR and 48 without AMR). The ratio of Tfr cells in renal graft tissues and peripheral blood of AMR patients significantly decreased, while the ratio of IL-21-producing Tfh cells (Tfh2 and Tfh17) significantly increased, compared to non-AMR patients. When tested in functional assays, Tfr cells from both AMR and non-AMR patients exerted equivalent inhibitory function. Tfr cell transplantation or CTLA-4 virus transfection could significantly inhibit IL-21 secretion from Tfh cells of these patients, further suppress the proliferation and differentiation of B cells. CTLA-4 blocking, IL-10 and TGF-β neutralization could partially weaken such inhibitory effect of Tfr cells. Besides, our study found that sirolimus reduced the ratio of Tfr cells, while cyclosporine and tacrolimus had no significant effect on Tfr cells. In a word, renal transplant patients with AMR have low proportion of Tfr cells but these cell exerted normal function.Wen ChenJian BaiHaiyan HuangLili BiXiangrui KongYu GaoYong HanLi XiaoBingyi ShiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wen Chen Jian Bai Haiyan Huang Lili Bi Xiangrui Kong Yu Gao Yong Han Li Xiao Bingyi Shi Low proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection
description	Abstract Follicular regulatory T (Tfr) cell can effectively regulate humoral immunity, but its function and mechanism in antibody-mediated rejection (AMR) after organ transplantation remains unclear. Here we detected follicular helper T (Tfh) cell subsets in 88 renal transplant patients with chronic renal allograft dysfunction (40 with AMR and 48 without AMR). The ratio of Tfr cells in renal graft tissues and peripheral blood of AMR patients significantly decreased, while the ratio of IL-21-producing Tfh cells (Tfh2 and Tfh17) significantly increased, compared to non-AMR patients. When tested in functional assays, Tfr cells from both AMR and non-AMR patients exerted equivalent inhibitory function. Tfr cell transplantation or CTLA-4 virus transfection could significantly inhibit IL-21 secretion from Tfh cells of these patients, further suppress the proliferation and differentiation of B cells. CTLA-4 blocking, IL-10 and TGF-β neutralization could partially weaken such inhibitory effect of Tfr cells. Besides, our study found that sirolimus reduced the ratio of Tfr cells, while cyclosporine and tacrolimus had no significant effect on Tfr cells. In a word, renal transplant patients with AMR have low proportion of Tfr cells but these cell exerted normal function.
format	article
author	Wen Chen Jian Bai Haiyan Huang Lili Bi Xiangrui Kong Yu Gao Yong Han Li Xiao Bingyi Shi
author_facet	Wen Chen Jian Bai Haiyan Huang Lili Bi Xiangrui Kong Yu Gao Yong Han Li Xiao Bingyi Shi
author_sort	Wen Chen
title	Low proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection
title_short	Low proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection
title_full	Low proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection
title_fullStr	Low proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection
title_full_unstemmed	Low proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection
title_sort	low proportion of follicular regulatory t cell in renal transplant patients with chronic antibody-mediated rejection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/af570329ae904844b525a77d7f204a90
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Low proportion of follicular regulatory T cell in renal transplant patients with chronic antibody-mediated rejection

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