Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer

Islam Ali Abdel Raheem,1 Amro Abdul Razek,1 Abeer Abdelaziz Elgendy,1 Noha Mohamed Saleh,2 Mona Ibrahem Shaaban,3 Faten K Abd El-Hady4 1Department of Endodontics, Faculty of Dentistry, Mansoura University, Mansoura, Egypt; 2Department of Pharmaceutics, Faculty of Pharmacy, Mansoura University, Manso...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Abdel Raheem IA, Abdul Razek A, Elgendy AA, Saleh NM, Shaaban MI, Abd El-Hady FK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	propolis sealer polymeric nanoparticles PLGA in vitro cytotoxicity antimicrobial activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/af5b469586ef46ee97919c73d574177b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:af5b469586ef46ee97919c73d574177b
record_format	dspace
spelling	oai:doaj.org-article:af5b469586ef46ee97919c73d574177b2021-12-02T06:29:11ZDesign, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer1178-2013https://doaj.org/article/af5b469586ef46ee97919c73d574177b2019-10-01T00:00:00Zhttps://www.dovepress.com/design-evaluation-and-antimicrobial-activity-of-egyptian-propolis-load-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Islam Ali Abdel Raheem,1 Amro Abdul Razek,1 Abeer Abdelaziz Elgendy,1 Noha Mohamed Saleh,2 Mona Ibrahem Shaaban,3 Faten K Abd El-Hady4 1Department of Endodontics, Faculty of Dentistry, Mansoura University, Mansoura, Egypt; 2Department of Pharmaceutics, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; 3Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; 4Chemistry of Natural and Microbial Products Department, National Research Centre, Giza, EgyptCorrespondence: Noha Mohamed SalehDepartment of Pharmaceutics, Faculty of Pharmacy, Mansoura University, Gomhoreyah Street, Mansoura 35516, EgyptTel/fax +2 050 2247496Email nunu_ramy@yahoo.comBackground: Propolis is a unique natural adhesive product collected by honeybees. It contains a diversity of bioactive compounds with reported functional properties such as antioxidants, antibacterial, antifungal, anti-inflammatory, antiviral and anticancer activity. Dental caries is a worldwide problem that caused by microbial growth usually progress from tooth enamel to the underlying pulpal tissues and root canal. This situation could be controlled by a sequence of steps to remove microorganisms and fill root canal with a suitable long-lasting root canal sealer. Unfortunately, leachable and degradation products of the currently used sealers compromised their antimicrobial activity by inflammatory modulation associated with irritation and toxicity of periapical tissues.Materials and methods: Hence, propolis was selected to be designed as a natural root canal sealer due to its amazing functional properties. Moreover, its handling properties were enhanced and potentiated by its incorporation in polymeric nanoparticles (NPs). Frist, propolis was collected, extracted and analyzed for its bioactive compounds. After that, propolis-loaded NPs of PLGA (ProE-loaded NPs) were developed and fully characterized regarding physicochemical properties, in vitro release and in vitro cytotoxicity. Then, root canal sealers were fabricated and assayed for their antimicrobial activity. Both cytotoxicity and antimicrobial activity were compared to those of a model sealer; AH Plus®.Results: The results revealed that spherical nanoscopic NPs with narrow size distribution were obtained. ProE-loaded NPs exhibited accepted entrapment efficiency (>80) and prolonged release. In vitro cytotoxicity study confirmed the safety of ProE-loaded NPs. Also, the developed sealers showed antimicrobial activity versus bacterial strains of Enterococcus faecalis and Streptococcus mutans and antifungal activity against Candida albicans.Conclusion: ProE-loaded NPs could be incorporated in and represented as a root canal sealer with prolonged release and enhanced cytocompatibility as well as antimicrobial activities.Keywords: propolis, sealer, polymeric nanoparticles, PLGA, in vitro cytotoxicity, antimicrobial activityAbdel Raheem IAAbdul Razek AElgendy AASaleh NMShaaban MIAbd El-Hady FKDove Medical Pressarticlepropolissealerpolymeric nanoparticlesPLGAin vitro cytotoxicityantimicrobial activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8379-8398 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	propolis sealer polymeric nanoparticles PLGA in vitro cytotoxicity antimicrobial activity Medicine (General) R5-920
spellingShingle	propolis sealer polymeric nanoparticles PLGA in vitro cytotoxicity antimicrobial activity Medicine (General) R5-920 Abdel Raheem IA Abdul Razek A Elgendy AA Saleh NM Shaaban MI Abd El-Hady FK Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer
description	Islam Ali Abdel Raheem,1 Amro Abdul Razek,1 Abeer Abdelaziz Elgendy,1 Noha Mohamed Saleh,2 Mona Ibrahem Shaaban,3 Faten K Abd El-Hady4 1Department of Endodontics, Faculty of Dentistry, Mansoura University, Mansoura, Egypt; 2Department of Pharmaceutics, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; 3Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; 4Chemistry of Natural and Microbial Products Department, National Research Centre, Giza, EgyptCorrespondence: Noha Mohamed SalehDepartment of Pharmaceutics, Faculty of Pharmacy, Mansoura University, Gomhoreyah Street, Mansoura 35516, EgyptTel/fax +2 050 2247496Email nunu_ramy@yahoo.comBackground: Propolis is a unique natural adhesive product collected by honeybees. It contains a diversity of bioactive compounds with reported functional properties such as antioxidants, antibacterial, antifungal, anti-inflammatory, antiviral and anticancer activity. Dental caries is a worldwide problem that caused by microbial growth usually progress from tooth enamel to the underlying pulpal tissues and root canal. This situation could be controlled by a sequence of steps to remove microorganisms and fill root canal with a suitable long-lasting root canal sealer. Unfortunately, leachable and degradation products of the currently used sealers compromised their antimicrobial activity by inflammatory modulation associated with irritation and toxicity of periapical tissues.Materials and methods: Hence, propolis was selected to be designed as a natural root canal sealer due to its amazing functional properties. Moreover, its handling properties were enhanced and potentiated by its incorporation in polymeric nanoparticles (NPs). Frist, propolis was collected, extracted and analyzed for its bioactive compounds. After that, propolis-loaded NPs of PLGA (ProE-loaded NPs) were developed and fully characterized regarding physicochemical properties, in vitro release and in vitro cytotoxicity. Then, root canal sealers were fabricated and assayed for their antimicrobial activity. Both cytotoxicity and antimicrobial activity were compared to those of a model sealer; AH Plus®.Results: The results revealed that spherical nanoscopic NPs with narrow size distribution were obtained. ProE-loaded NPs exhibited accepted entrapment efficiency (>80) and prolonged release. In vitro cytotoxicity study confirmed the safety of ProE-loaded NPs. Also, the developed sealers showed antimicrobial activity versus bacterial strains of Enterococcus faecalis and Streptococcus mutans and antifungal activity against Candida albicans.Conclusion: ProE-loaded NPs could be incorporated in and represented as a root canal sealer with prolonged release and enhanced cytocompatibility as well as antimicrobial activities.Keywords: propolis, sealer, polymeric nanoparticles, PLGA, in vitro cytotoxicity, antimicrobial activity
format	article
author	Abdel Raheem IA Abdul Razek A Elgendy AA Saleh NM Shaaban MI Abd El-Hady FK
author_facet	Abdel Raheem IA Abdul Razek A Elgendy AA Saleh NM Shaaban MI Abd El-Hady FK
author_sort	Abdel Raheem IA
title	Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer
title_short	Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer
title_full	Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer
title_fullStr	Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer
title_full_unstemmed	Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer
title_sort	design, evaluation and antimicrobial activity of egyptian propolis-loaded nanoparticles: intrinsic role as a novel and naturally based root canal nanosealer
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/af5b469586ef46ee97919c73d574177b
work_keys_str_mv	AT abdelraheemia designevaluationandantimicrobialactivityofegyptianpropolisloadednanoparticlesintrinsicroleasanovelandnaturallybasedrootcanalnanosealer AT abdulrazeka designevaluationandantimicrobialactivityofegyptianpropolisloadednanoparticlesintrinsicroleasanovelandnaturallybasedrootcanalnanosealer AT elgendyaa designevaluationandantimicrobialactivityofegyptianpropolisloadednanoparticlesintrinsicroleasanovelandnaturallybasedrootcanalnanosealer AT salehnm designevaluationandantimicrobialactivityofegyptianpropolisloadednanoparticlesintrinsicroleasanovelandnaturallybasedrootcanalnanosealer AT shaabanmi designevaluationandantimicrobialactivityofegyptianpropolisloadednanoparticlesintrinsicroleasanovelandnaturallybasedrootcanalnanosealer AT abdelhadyfk designevaluationandantimicrobialactivityofegyptianpropolisloadednanoparticlesintrinsicroleasanovelandnaturallybasedrootcanalnanosealer
_version_	1718399895145021440

Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer

Ejemplares similares