PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction

Cardiac development is a complex process that is strictly controlled by various factors, including PcG protein complexes. Several studies have reported the critical role of PRC2 in cardiogenesis. However, little is known about the regulation mechanism of PRC1 in embryonic heart development. To gain...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xixia Peng, Gang Feng, Yanyong Zhang, Yuhua Sun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Rnf2 PRC1 cardiac contraction sarcomere assembly cardiac conduction system Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/af5bfb32ca844b9aa99deb152e8168f9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:af5bfb32ca844b9aa99deb152e8168f9
record_format	dspace
spelling	oai:doaj.org-article:af5bfb32ca844b9aa99deb152e8168f92021-11-11T16:50:39ZPRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction10.3390/ijms2221113681422-00671661-6596https://doaj.org/article/af5bfb32ca844b9aa99deb152e8168f92021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11368https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cardiac development is a complex process that is strictly controlled by various factors, including PcG protein complexes. Several studies have reported the critical role of PRC2 in cardiogenesis. However, little is known about the regulation mechanism of PRC1 in embryonic heart development. To gain more insight into the mechanistic role of PRC1 in cardiogenesis, we generated a PRC1 loss-of-function zebrafish line by using the CRISPR/Cas9 system targeting <i>rnf2</i>, a gene encoding the core subunit shared by all PRC1 subfamilies. Our results revealed that Rnf2 is not involved in cardiomyocyte differentiation and heart tube formation, but that it is crucial to maintaining regular cardiac contraction. Further analysis suggested that Rnf2 loss-of-function disrupted cardiac sarcomere assembly through the ectopic activation of non-cardiac sarcomere genes in the developing heart. Meanwhile, Rnf2 deficiency disrupts the construction of the atrioventricular canal and the sinoatrial node by modulating the expression of <i>bmp4</i> and other atrioventricular canal marker genes, leading to an impaired cardiac conduction system. The disorganized cardiac sarcomere and defective cardiac conduction system together contribute to defective cardiac contraction. Our results emphasize the critical role of PRC1 in the cardiac development.Xixia PengGang FengYanyong ZhangYuhua SunMDPI AGarticleRnf2PRC1cardiac contractionsarcomere assemblycardiac conduction systemBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11368, p 11368 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rnf2 PRC1 cardiac contraction sarcomere assembly cardiac conduction system Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	Rnf2 PRC1 cardiac contraction sarcomere assembly cardiac conduction system Biology (General) QH301-705.5 Chemistry QD1-999 Xixia Peng Gang Feng Yanyong Zhang Yuhua Sun PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction
description	Cardiac development is a complex process that is strictly controlled by various factors, including PcG protein complexes. Several studies have reported the critical role of PRC2 in cardiogenesis. However, little is known about the regulation mechanism of PRC1 in embryonic heart development. To gain more insight into the mechanistic role of PRC1 in cardiogenesis, we generated a PRC1 loss-of-function zebrafish line by using the CRISPR/Cas9 system targeting <i>rnf2</i>, a gene encoding the core subunit shared by all PRC1 subfamilies. Our results revealed that Rnf2 is not involved in cardiomyocyte differentiation and heart tube formation, but that it is crucial to maintaining regular cardiac contraction. Further analysis suggested that Rnf2 loss-of-function disrupted cardiac sarcomere assembly through the ectopic activation of non-cardiac sarcomere genes in the developing heart. Meanwhile, Rnf2 deficiency disrupts the construction of the atrioventricular canal and the sinoatrial node by modulating the expression of <i>bmp4</i> and other atrioventricular canal marker genes, leading to an impaired cardiac conduction system. The disorganized cardiac sarcomere and defective cardiac conduction system together contribute to defective cardiac contraction. Our results emphasize the critical role of PRC1 in the cardiac development.
format	article
author	Xixia Peng Gang Feng Yanyong Zhang Yuhua Sun
author_facet	Xixia Peng Gang Feng Yanyong Zhang Yuhua Sun
author_sort	Xixia Peng
title	PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction
title_short	PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction
title_full	PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction
title_fullStr	PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction
title_full_unstemmed	PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction
title_sort	prc1 stabilizes cardiac contraction by regulating cardiac sarcomere assembly and cardiac conduction system construction
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/af5bfb32ca844b9aa99deb152e8168f9
work_keys_str_mv	AT xixiapeng prc1stabilizescardiaccontractionbyregulatingcardiacsarcomereassemblyandcardiacconductionsystemconstruction AT gangfeng prc1stabilizescardiaccontractionbyregulatingcardiacsarcomereassemblyandcardiacconductionsystemconstruction AT yanyongzhang prc1stabilizescardiaccontractionbyregulatingcardiacsarcomereassemblyandcardiacconductionsystemconstruction AT yuhuasun prc1stabilizescardiaccontractionbyregulatingcardiacsarcomereassemblyandcardiacconductionsystemconstruction
_version_	1718432259131834368

PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction

Ejemplares similares