Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.

<h4>Background</h4>Development of T-cells based-Interferon gamma (IFNgamma) assays has offered new possibilities for the diagnosis of latent tuberculosis infection (LTBI) and active disease in adults. Few studies have been performed in children, none in France. With reference to the publ...

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Autores principales: Jean-Louis Herrmann, Marie Belloy, Raphael Porcher, Nancy Simonney, Rola Aboutaam, Muriel Lebourgeois, Joel Gaudelus, Laure De Losangeles, Katarina Chadelat, Pierre Scheinmann, Nicole Beydon, Brigitte Fauroux, Martine Bingen, Mustapha Terki, Dominique Barraud, Philippe Cruaud, Catherine Offredo, Agnes Ferroni, Patrick Berche, Didier Moissenet, Hoang Vuthien, Catherine Doit, Edouard Bingen, Philippe Henri Lagrange
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Publicado: Public Library of Science (PLoS) 2009
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spelling oai:doaj.org-article:af5dde56ae3b40518968ae1d3c7123ec2021-11-25T06:17:52ZTemporal dynamics of interferon gamma responses in children evaluated for tuberculosis.1932-620310.1371/journal.pone.0004130https://doaj.org/article/af5dde56ae3b40518968ae1d3c7123ec2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19125189/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Development of T-cells based-Interferon gamma (IFNgamma) assays has offered new possibilities for the diagnosis of latent tuberculosis infection (LTBI) and active disease in adults. Few studies have been performed in children, none in France. With reference to the published data on childhood TB epidemiology in the Paris and Ile de France Region, we considered it important to evaluate the performance of IGRA (QuantiFERON TB Gold In Tube(R), QF-TB-IT) in the diagnosis and the follow-up through treatment of LTBI and active TB in a cohort of French children.<h4>Methodology/principal findings</h4>131 children were recruited during a prospective and multicentre study (October 2005 and May 2007; Ethical Committee St Louis Hospital, Paris, study number 2005/32). Children were sampled at day 0, 10, 30, 60 (except Healthy Contacts, HC) and 90 for LTBI and HC, and a further day 120, and day 180 for active TB children. Median age was 7.4 years, with 91% of the children BCG vaccinated. LTBI and active TB children undergoing therapy produced significant higher IFNgamma values after 10 days of treatment (p = 0.035). In addition, IFNgamma values were significantly lower at the end of treatment compared to IFNgamma values at day 0, although the number of positive patients was not significantly different between day 0 and end of treatment.<h4>Conclusions/ significance</h4>By following quantitative IFNgamma values in each enrolled child with LTBI or active TB and receiving treatment, we were able to detect an increase in the IFNgamma response at day 10 of treatment which might allow the confirmation of a diagnosis. In addition, a decline in IFNgamma values during treatment makes it possible for clinicians to monitor the effect of preventive or curative therapy.Jean-Louis HerrmannMarie BelloyRaphael PorcherNancy SimonneyRola AboutaamMuriel LebourgeoisJoel GaudelusLaure De LosangelesKatarina ChadelatPierre ScheinmannNicole BeydonBrigitte FaurouxMartine BingenMustapha TerkiDominique BarraudPhilippe CruaudCatherine OffredoAgnes FerroniPatrick BercheDidier MoissenetHoang VuthienCatherine DoitEdouard BingenPhilippe Henri LagrangePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 1, p e4130 (2009)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jean-Louis Herrmann
Marie Belloy
Raphael Porcher
Nancy Simonney
Rola Aboutaam
Muriel Lebourgeois
Joel Gaudelus
Laure De Losangeles
Katarina Chadelat
Pierre Scheinmann
Nicole Beydon
Brigitte Fauroux
Martine Bingen
Mustapha Terki
Dominique Barraud
Philippe Cruaud
Catherine Offredo
Agnes Ferroni
Patrick Berche
Didier Moissenet
Hoang Vuthien
Catherine Doit
Edouard Bingen
Philippe Henri Lagrange
Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.
description <h4>Background</h4>Development of T-cells based-Interferon gamma (IFNgamma) assays has offered new possibilities for the diagnosis of latent tuberculosis infection (LTBI) and active disease in adults. Few studies have been performed in children, none in France. With reference to the published data on childhood TB epidemiology in the Paris and Ile de France Region, we considered it important to evaluate the performance of IGRA (QuantiFERON TB Gold In Tube(R), QF-TB-IT) in the diagnosis and the follow-up through treatment of LTBI and active TB in a cohort of French children.<h4>Methodology/principal findings</h4>131 children were recruited during a prospective and multicentre study (October 2005 and May 2007; Ethical Committee St Louis Hospital, Paris, study number 2005/32). Children were sampled at day 0, 10, 30, 60 (except Healthy Contacts, HC) and 90 for LTBI and HC, and a further day 120, and day 180 for active TB children. Median age was 7.4 years, with 91% of the children BCG vaccinated. LTBI and active TB children undergoing therapy produced significant higher IFNgamma values after 10 days of treatment (p = 0.035). In addition, IFNgamma values were significantly lower at the end of treatment compared to IFNgamma values at day 0, although the number of positive patients was not significantly different between day 0 and end of treatment.<h4>Conclusions/ significance</h4>By following quantitative IFNgamma values in each enrolled child with LTBI or active TB and receiving treatment, we were able to detect an increase in the IFNgamma response at day 10 of treatment which might allow the confirmation of a diagnosis. In addition, a decline in IFNgamma values during treatment makes it possible for clinicians to monitor the effect of preventive or curative therapy.
format article
author Jean-Louis Herrmann
Marie Belloy
Raphael Porcher
Nancy Simonney
Rola Aboutaam
Muriel Lebourgeois
Joel Gaudelus
Laure De Losangeles
Katarina Chadelat
Pierre Scheinmann
Nicole Beydon
Brigitte Fauroux
Martine Bingen
Mustapha Terki
Dominique Barraud
Philippe Cruaud
Catherine Offredo
Agnes Ferroni
Patrick Berche
Didier Moissenet
Hoang Vuthien
Catherine Doit
Edouard Bingen
Philippe Henri Lagrange
author_facet Jean-Louis Herrmann
Marie Belloy
Raphael Porcher
Nancy Simonney
Rola Aboutaam
Muriel Lebourgeois
Joel Gaudelus
Laure De Losangeles
Katarina Chadelat
Pierre Scheinmann
Nicole Beydon
Brigitte Fauroux
Martine Bingen
Mustapha Terki
Dominique Barraud
Philippe Cruaud
Catherine Offredo
Agnes Ferroni
Patrick Berche
Didier Moissenet
Hoang Vuthien
Catherine Doit
Edouard Bingen
Philippe Henri Lagrange
author_sort Jean-Louis Herrmann
title Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.
title_short Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.
title_full Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.
title_fullStr Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.
title_full_unstemmed Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.
title_sort temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.
publisher Public Library of Science (PLoS)
publishDate 2009
url https://doaj.org/article/af5dde56ae3b40518968ae1d3c7123ec
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