Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice

Abstract Postoperative peritonitis is characterized by a more severe clinical course than other forms of secondary peritonitis. The pathophysiological mechanisms behind this phenomenon are incompletely understood. This study used an innovative model to investigate these mechanisms, combining the mod...

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Autores principales:	Jonas Menz, Laura Hundt, Tobias Schulze, Katrin Schmoeckel, Pia Menges, Grazyna Domanska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af5e1223a525438b83ddc04791509c00
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spelling	oai:doaj.org-article:af5e1223a525438b83ddc04791509c002021-12-02T19:06:35ZIncreased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice10.1038/s41598-021-95592-52045-2322https://doaj.org/article/af5e1223a525438b83ddc04791509c002021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95592-5https://doaj.org/toc/2045-2322Abstract Postoperative peritonitis is characterized by a more severe clinical course than other forms of secondary peritonitis. The pathophysiological mechanisms behind this phenomenon are incompletely understood. This study used an innovative model to investigate these mechanisms, combining the models of murine Colon Ascendens Stent Peritonitis (CASP) and Surgically induced Immune Dysfunction (SID). Moreover, the influence of the previously described anti-inflammatory reflex transmitted by the vagal nerve was characterized. SID alone, or 3 days before CASP were performed in female C57BL/6 N mice. Subdiaphragmatic vagotomy was performed six days before SID with following CASP. The immune status was assessed by FACS analysis and measurement of cytokines. Local intestinal inflammatory changes were characterized by immunohistochemistry. Mortality was increased in CASP animals previously subjected to SID. Subclinical bacteremia occurred after SID, and an immunosuppressive milieu occurred secondary to SID just before the induction of CASP. Previous SID modified the pattern of intestinal inflammation induced by CASP. Subdiaphragmatic vagotomy had no influence on sepsis mortality in our model of postoperative peritonitis. Our results indicate a surgery-induced inflammation of the small intestine and the peritoneal cavity with bacterial translocation, which led to immune dysfunction and consequently to a more severe peritonitis.Jonas MenzLaura HundtTobias SchulzeKatrin SchmoeckelPia MengesGrazyna DomanskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonas Menz Laura Hundt Tobias Schulze Katrin Schmoeckel Pia Menges Grazyna Domanska Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice
description	Abstract Postoperative peritonitis is characterized by a more severe clinical course than other forms of secondary peritonitis. The pathophysiological mechanisms behind this phenomenon are incompletely understood. This study used an innovative model to investigate these mechanisms, combining the models of murine Colon Ascendens Stent Peritonitis (CASP) and Surgically induced Immune Dysfunction (SID). Moreover, the influence of the previously described anti-inflammatory reflex transmitted by the vagal nerve was characterized. SID alone, or 3 days before CASP were performed in female C57BL/6 N mice. Subdiaphragmatic vagotomy was performed six days before SID with following CASP. The immune status was assessed by FACS analysis and measurement of cytokines. Local intestinal inflammatory changes were characterized by immunohistochemistry. Mortality was increased in CASP animals previously subjected to SID. Subclinical bacteremia occurred after SID, and an immunosuppressive milieu occurred secondary to SID just before the induction of CASP. Previous SID modified the pattern of intestinal inflammation induced by CASP. Subdiaphragmatic vagotomy had no influence on sepsis mortality in our model of postoperative peritonitis. Our results indicate a surgery-induced inflammation of the small intestine and the peritoneal cavity with bacterial translocation, which led to immune dysfunction and consequently to a more severe peritonitis.
format	article
author	Jonas Menz Laura Hundt Tobias Schulze Katrin Schmoeckel Pia Menges Grazyna Domanska
author_facet	Jonas Menz Laura Hundt Tobias Schulze Katrin Schmoeckel Pia Menges Grazyna Domanska
author_sort	Jonas Menz
title	Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice
title_short	Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice
title_full	Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice
title_fullStr	Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice
title_full_unstemmed	Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice
title_sort	increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/af5e1223a525438b83ddc04791509c00
work_keys_str_mv	AT jonasmenz increasedmortalityandalteredlocalimmuneresponseinsecondaryperitonitisafterpreviousvisceraloperationsinmice AT laurahundt increasedmortalityandalteredlocalimmuneresponseinsecondaryperitonitisafterpreviousvisceraloperationsinmice AT tobiasschulze increasedmortalityandalteredlocalimmuneresponseinsecondaryperitonitisafterpreviousvisceraloperationsinmice AT katrinschmoeckel increasedmortalityandalteredlocalimmuneresponseinsecondaryperitonitisafterpreviousvisceraloperationsinmice AT piamenges increasedmortalityandalteredlocalimmuneresponseinsecondaryperitonitisafterpreviousvisceraloperationsinmice AT grazynadomanska increasedmortalityandalteredlocalimmuneresponseinsecondaryperitonitisafterpreviousvisceraloperationsinmice
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Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice

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