Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection

Objective: Identification of otomycotic fungi using matrix-assisted laser desorption ionization (MALDI) time of flight (TOF) mass spectroscopy (MS) and to quantify pervasive errors with Bayes rule; values of sensitivity and specificity of culture test and MALDI-TOF-MS method are quantified. Method:...

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Autores principales:	Sibanarayan Rath, Saumya Ranjan Das, Rabindra Nath Padhy
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2019
Materias:	Otorhinolaryngology RF1-547 Surgery RD1-811
Acceso en línea:	https://doaj.org/article/af6bdd769f4f492fae72e583006d7a51
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spelling	oai:doaj.org-article:af6bdd769f4f492fae72e583006d7a512021-12-02T13:21:24ZBayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection2095-881110.1016/j.wjorl.2018.03.006https://doaj.org/article/af6bdd769f4f492fae72e583006d7a512019-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2095881117300409https://doaj.org/toc/2095-8811Objective: Identification of otomycotic fungi using matrix-assisted laser desorption ionization (MALDI) time of flight (TOF) mass spectroscopy (MS) and to quantify pervasive errors with Bayes rule; values of sensitivity and specificity of culture test and MALDI-TOF-MS method are quantified. Method: Fungi cultured ear discharge samples were identified with culture test and MALDI-TOF-MS system. Minimum inhibitory concentration (MIC) or MEC (minimum effective concentration) for 6 antifungals were determined by antifungal susceptibility testing in vitro. With Bayes rule, sensitivity and specificity of both MALDI-TOF MS and culture test methods were computed. Results: Cultures yielded 42 fungal isolates which were confirmed as species (specified against each species) belonging to 8 genera, A. niger 22, Candida sp. 7, A. fumigatus 3, A. flavus 3, mixed Aspergillus sp. 3, Mucor sp. 2, Rhizopus sp. 1, and Scopulariopsis sp.1; and MALDI-TOF-MS system also confirmed those isolates. In vitro antifungal susceptibility testing with terms of MIC 50 and MIC 90, isolates fungi were highly susceptible to 6 antifungals; and caspofungin was the most active antifungal. The high value of specificity 84.6%, suggested a limited loss of confidence on the culture test at the absence of an infection, in comparison to MALDI-TOF-MS. Sensitivity of the culture test was 87.5%; this high figure strongly approves the culture test to be the dependable method for the otomycosis diagnosis, when the infection is stable. Conclusion: Based on sensitivity and specificity together the culture test could be done in face of the gold-standard MALDI-TOF-MS system the estimating both methods. Keywords: Otomycosis, MALDI-TOF-MS system, Identification at species level, Sensitivity, SpecificitySibanarayan RathSaumya Ranjan DasRabindra Nath PadhyKeAi Communications Co., Ltd.articleOtorhinolaryngologyRF1-547SurgeryRD1-811ENWorld Journal of Otorhinolaryngology-Head and Neck Surgery, Vol 5, Iss 1, Pp 6-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Otorhinolaryngology RF1-547 Surgery RD1-811
spellingShingle	Otorhinolaryngology RF1-547 Surgery RD1-811 Sibanarayan Rath Saumya Ranjan Das Rabindra Nath Padhy Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection
description	Objective: Identification of otomycotic fungi using matrix-assisted laser desorption ionization (MALDI) time of flight (TOF) mass spectroscopy (MS) and to quantify pervasive errors with Bayes rule; values of sensitivity and specificity of culture test and MALDI-TOF-MS method are quantified. Method: Fungi cultured ear discharge samples were identified with culture test and MALDI-TOF-MS system. Minimum inhibitory concentration (MIC) or MEC (minimum effective concentration) for 6 antifungals were determined by antifungal susceptibility testing in vitro. With Bayes rule, sensitivity and specificity of both MALDI-TOF MS and culture test methods were computed. Results: Cultures yielded 42 fungal isolates which were confirmed as species (specified against each species) belonging to 8 genera, A. niger 22, Candida sp. 7, A. fumigatus 3, A. flavus 3, mixed Aspergillus sp. 3, Mucor sp. 2, Rhizopus sp. 1, and Scopulariopsis sp.1; and MALDI-TOF-MS system also confirmed those isolates. In vitro antifungal susceptibility testing with terms of MIC 50 and MIC 90, isolates fungi were highly susceptible to 6 antifungals; and caspofungin was the most active antifungal. The high value of specificity 84.6%, suggested a limited loss of confidence on the culture test at the absence of an infection, in comparison to MALDI-TOF-MS. Sensitivity of the culture test was 87.5%; this high figure strongly approves the culture test to be the dependable method for the otomycosis diagnosis, when the infection is stable. Conclusion: Based on sensitivity and specificity together the culture test could be done in face of the gold-standard MALDI-TOF-MS system the estimating both methods. Keywords: Otomycosis, MALDI-TOF-MS system, Identification at species level, Sensitivity, Specificity
format	article
author	Sibanarayan Rath Saumya Ranjan Das Rabindra Nath Padhy
author_facet	Sibanarayan Rath Saumya Ranjan Das Rabindra Nath Padhy
author_sort	Sibanarayan Rath
title	Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection
title_short	Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection
title_full	Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection
title_fullStr	Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection
title_full_unstemmed	Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection
title_sort	bayesian analysis of two methods maldi-tof-ms system and culture test in otomycosis infection
publisher	KeAi Communications Co., Ltd.
publishDate	2019
url	https://doaj.org/article/af6bdd769f4f492fae72e583006d7a51
work_keys_str_mv	AT sibanarayanrath bayesiananalysisoftwomethodsmalditofmssystemandculturetestinotomycosisinfection AT saumyaranjandas bayesiananalysisoftwomethodsmalditofmssystemandculturetestinotomycosisinfection AT rabindranathpadhy bayesiananalysisoftwomethodsmalditofmssystemandculturetestinotomycosisinfection
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Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection

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