Standard screening methods underreport AAV-mediated transduction and gene editing

Conventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.

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Autores principales:	Jonathan F. Lang, Sushila A. Toulmin, Kasey L. Brida, Laurence C. Eisenlohr, Beverly L. Davidson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/af6cb7bdc8fb4782a4723ed186a60397
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spelling	oai:doaj.org-article:af6cb7bdc8fb4782a4723ed186a603972021-12-02T17:01:46ZStandard screening methods underreport AAV-mediated transduction and gene editing10.1038/s41467-019-11321-72041-1723https://doaj.org/article/af6cb7bdc8fb4782a4723ed186a603972019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11321-7https://doaj.org/toc/2041-1723Conventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.Jonathan F. LangSushila A. ToulminKasey L. BridaLaurence C. EisenlohrBeverly L. DavidsonNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jonathan F. Lang Sushila A. Toulmin Kasey L. Brida Laurence C. Eisenlohr Beverly L. Davidson Standard screening methods underreport AAV-mediated transduction and gene editing
description	Conventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.
format	article
author	Jonathan F. Lang Sushila A. Toulmin Kasey L. Brida Laurence C. Eisenlohr Beverly L. Davidson
author_facet	Jonathan F. Lang Sushila A. Toulmin Kasey L. Brida Laurence C. Eisenlohr Beverly L. Davidson
author_sort	Jonathan F. Lang
title	Standard screening methods underreport AAV-mediated transduction and gene editing
title_short	Standard screening methods underreport AAV-mediated transduction and gene editing
title_full	Standard screening methods underreport AAV-mediated transduction and gene editing
title_fullStr	Standard screening methods underreport AAV-mediated transduction and gene editing
title_full_unstemmed	Standard screening methods underreport AAV-mediated transduction and gene editing
title_sort	standard screening methods underreport aav-mediated transduction and gene editing
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/af6cb7bdc8fb4782a4723ed186a60397
work_keys_str_mv	AT jonathanflang standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting AT sushilaatoulmin standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting AT kaseylbrida standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting AT laurenceceisenlohr standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting AT beverlyldavidson standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting
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Standard screening methods underreport AAV-mediated transduction and gene editing

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