CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites

Global inhibition of NHEJ factors has been one strategy to improve CRISPR-Cas9 mediated HDR. Here the authors fuse a dominant-negative mutant of 53BP1 to Cas9 to enhance HDR frequency, reduce NHEJ specifically at the Cas9 cut sites, and reduce the toxicity associated with global NHEJ inhibition.

Guardado en:

Detalles Bibliográficos
Autores principales:	Rajeswari Jayavaradhan, Devin M. Pillis, Michael Goodman, Fan Zhang, Yue Zhang, Paul R. Andreassen, Punam Malik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/af6eb1ef193d4ac7958e34f1c42a6fa1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:af6eb1ef193d4ac7958e34f1c42a6fa1
record_format	dspace
spelling	oai:doaj.org-article:af6eb1ef193d4ac7958e34f1c42a6fa12021-12-02T14:35:44ZCRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites10.1038/s41467-019-10735-72041-1723https://doaj.org/article/af6eb1ef193d4ac7958e34f1c42a6fa12019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10735-7https://doaj.org/toc/2041-1723Global inhibition of NHEJ factors has been one strategy to improve CRISPR-Cas9 mediated HDR. Here the authors fuse a dominant-negative mutant of 53BP1 to Cas9 to enhance HDR frequency, reduce NHEJ specifically at the Cas9 cut sites, and reduce the toxicity associated with global NHEJ inhibition.Rajeswari JayavaradhanDevin M. PillisMichael GoodmanFan ZhangYue ZhangPaul R. AndreassenPunam MalikNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Rajeswari Jayavaradhan Devin M. Pillis Michael Goodman Fan Zhang Yue Zhang Paul R. Andreassen Punam Malik CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
description	Global inhibition of NHEJ factors has been one strategy to improve CRISPR-Cas9 mediated HDR. Here the authors fuse a dominant-negative mutant of 53BP1 to Cas9 to enhance HDR frequency, reduce NHEJ specifically at the Cas9 cut sites, and reduce the toxicity associated with global NHEJ inhibition.
format	article
author	Rajeswari Jayavaradhan Devin M. Pillis Michael Goodman Fan Zhang Yue Zhang Paul R. Andreassen Punam Malik
author_facet	Rajeswari Jayavaradhan Devin M. Pillis Michael Goodman Fan Zhang Yue Zhang Paul R. Andreassen Punam Malik
author_sort	Rajeswari Jayavaradhan
title	CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
title_short	CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
title_full	CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
title_fullStr	CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
title_full_unstemmed	CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
title_sort	crispr-cas9 fusion to dominant-negative 53bp1 enhances hdr and inhibits nhej specifically at cas9 target sites
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/af6eb1ef193d4ac7958e34f1c42a6fa1
work_keys_str_mv	AT rajeswarijayavaradhan crisprcas9fusiontodominantnegative53bp1enhanceshdrandinhibitsnhejspecificallyatcas9targetsites AT devinmpillis crisprcas9fusiontodominantnegative53bp1enhanceshdrandinhibitsnhejspecificallyatcas9targetsites AT michaelgoodman crisprcas9fusiontodominantnegative53bp1enhanceshdrandinhibitsnhejspecificallyatcas9targetsites AT fanzhang crisprcas9fusiontodominantnegative53bp1enhanceshdrandinhibitsnhejspecificallyatcas9targetsites AT yuezhang crisprcas9fusiontodominantnegative53bp1enhanceshdrandinhibitsnhejspecificallyatcas9targetsites AT paulrandreassen crisprcas9fusiontodominantnegative53bp1enhanceshdrandinhibitsnhejspecificallyatcas9targetsites AT punammalik crisprcas9fusiontodominantnegative53bp1enhanceshdrandinhibitsnhejspecificallyatcas9targetsites
_version_	1718391093671755776

CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites

Ejemplares similares