Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates

Abstract Despite the mitochondrial antiviral signalling protein (MAVS)-dependent RIG-I-like receptor (RLR) signalling pathway in the cytosol plays an indispensable role in the antiviral immunity of the host, surprising little is known in invertebrates. Here we characterized the major members of RLR...

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Autores principales:	Baoyu Huang, Linlin Zhang, Yishuai Du, Fei Xu, Li Li, Guofan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af75537375d24606867e7b29811ca0e7
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spelling	oai:doaj.org-article:af75537375d24606867e7b29811ca0e72021-12-02T15:06:14ZCharacterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates10.1038/s41598-017-08566-x2045-2322https://doaj.org/article/af75537375d24606867e7b29811ca0e72017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08566-xhttps://doaj.org/toc/2045-2322Abstract Despite the mitochondrial antiviral signalling protein (MAVS)-dependent RIG-I-like receptor (RLR) signalling pathway in the cytosol plays an indispensable role in the antiviral immunity of the host, surprising little is known in invertebrates. Here we characterized the major members of RLR pathway and investigated their signal transduction a Molluscs. We show that genes involved in RLR pathway were significantly induced during virus challenge, including CgRIG-I-1, CgMAVS, CgTRAF6 (TNF receptor-associated factor 6), and CgIRFs (interferon regulatory factors. Similar to human RIG-I, oyster RIG-I-1 could bind poly(I:C) directly in vitro and interact with oyster MAVS via its caspase activation and recruitment domains. We also show that transmembrane domain-dependent self-association of CgMAVS may be crucial for its signalling and that CgMAVS can recruit the downstream signalling molecule, TRAF6, which can subsequently activate NF-κB signal pathway. Moreover, oyster IRFs appeared to function downstream of CgMAVS and were able to activate the interferon β promoter and interferon stimulated response elements in mammalian cells. These results establish invertebrate MAVS-dependent RLR signalling for the first time and would be helpful for deciphering the antiviral mechanisms of invertebrates and understanding the development of the vertebrate RLR network.Baoyu HuangLinlin ZhangYishuai DuFei XuLi LiGuofan ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Baoyu Huang Linlin Zhang Yishuai Du Fei Xu Li Li Guofan Zhang Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
description	Abstract Despite the mitochondrial antiviral signalling protein (MAVS)-dependent RIG-I-like receptor (RLR) signalling pathway in the cytosol plays an indispensable role in the antiviral immunity of the host, surprising little is known in invertebrates. Here we characterized the major members of RLR pathway and investigated their signal transduction a Molluscs. We show that genes involved in RLR pathway were significantly induced during virus challenge, including CgRIG-I-1, CgMAVS, CgTRAF6 (TNF receptor-associated factor 6), and CgIRFs (interferon regulatory factors. Similar to human RIG-I, oyster RIG-I-1 could bind poly(I:C) directly in vitro and interact with oyster MAVS via its caspase activation and recruitment domains. We also show that transmembrane domain-dependent self-association of CgMAVS may be crucial for its signalling and that CgMAVS can recruit the downstream signalling molecule, TRAF6, which can subsequently activate NF-κB signal pathway. Moreover, oyster IRFs appeared to function downstream of CgMAVS and were able to activate the interferon β promoter and interferon stimulated response elements in mammalian cells. These results establish invertebrate MAVS-dependent RLR signalling for the first time and would be helpful for deciphering the antiviral mechanisms of invertebrates and understanding the development of the vertebrate RLR network.
format	article
author	Baoyu Huang Linlin Zhang Yishuai Du Fei Xu Li Li Guofan Zhang
author_facet	Baoyu Huang Linlin Zhang Yishuai Du Fei Xu Li Li Guofan Zhang
author_sort	Baoyu Huang
title	Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
title_short	Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
title_full	Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
title_fullStr	Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
title_full_unstemmed	Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
title_sort	characterization of the mollusc rig-i/mavs pathway reveals an archaic antiviral signalling framework in invertebrates
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/af75537375d24606867e7b29811ca0e7
work_keys_str_mv	AT baoyuhuang characterizationofthemolluscrigimavspathwayrevealsanarchaicantiviralsignallingframeworkininvertebrates AT linlinzhang characterizationofthemolluscrigimavspathwayrevealsanarchaicantiviralsignallingframeworkininvertebrates AT yishuaidu characterizationofthemolluscrigimavspathwayrevealsanarchaicantiviralsignallingframeworkininvertebrates AT feixu characterizationofthemolluscrigimavspathwayrevealsanarchaicantiviralsignallingframeworkininvertebrates AT lili characterizationofthemolluscrigimavspathwayrevealsanarchaicantiviralsignallingframeworkininvertebrates AT guofanzhang characterizationofthemolluscrigimavspathwayrevealsanarchaicantiviralsignallingframeworkininvertebrates
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Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates

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