Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.

Human induced pluripotent stem cells (hiPSCs) can differentiate into notochordal cell (NC)-like cells when cultured in the presence of natural porcine nucleus pulposus (NP) tissue matrix. The method promises massive production of high-quality, functional cells to treat degenerative intervertebral di...

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Autores principales:	Yongxing Liu, Mohamed N Rahaman, B Sonny Bal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af75ab5a2e624742a2522ac902f5bdfb
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spelling	oai:doaj.org-article:af75ab5a2e624742a2522ac902f5bdfb2021-11-25T06:07:31ZModulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.1932-620310.1371/journal.pone.0100885https://doaj.org/article/af75ab5a2e624742a2522ac902f5bdfb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25054208/?tool=EBIhttps://doaj.org/toc/1932-6203Human induced pluripotent stem cells (hiPSCs) can differentiate into notochordal cell (NC)-like cells when cultured in the presence of natural porcine nucleus pulposus (NP) tissue matrix. The method promises massive production of high-quality, functional cells to treat degenerative intervertebral discs (IVDs). Based on our previous work, we further examined the effect of cell-NP matrix contact and culture medium on the differentiation, and further assessed the functional differentiation ability of the generated NC-like. The study showed that direct contact between hiPSCs and NP matrix can promote the differentiation yield, whilst both the contact and non-contact cultures can generate functional NC-like cells. The generated NC-like cells are highly homogenous regarding the expression of notochordal marker genes. A culture medium containing a cocktail of growth factors (FGF, EGF, VEGF and IGF-1) also supported the notochordal differentiation in the presence of NP matrix. The NC-like cells showed excellent functional differentiation ability to generate NP-like tissue which was rich in aggrecan and collagen type II; and particularly, the proteoglycan to collagen content ratio was as high as 12.5-17.5 which represents a phenotype close to NP rather than hyaline cartilage. Collectively, the present study confirmed the effectiveness and flexibility of using natural NP tissue matrix to direct notochordal differentiation of hiPSCs, and the potential of using the generated NC-like cells for treating IVD degeneration.Yongxing LiuMohamed N RahamanB Sonny BalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e100885 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yongxing Liu Mohamed N Rahaman B Sonny Bal Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.
description	Human induced pluripotent stem cells (hiPSCs) can differentiate into notochordal cell (NC)-like cells when cultured in the presence of natural porcine nucleus pulposus (NP) tissue matrix. The method promises massive production of high-quality, functional cells to treat degenerative intervertebral discs (IVDs). Based on our previous work, we further examined the effect of cell-NP matrix contact and culture medium on the differentiation, and further assessed the functional differentiation ability of the generated NC-like. The study showed that direct contact between hiPSCs and NP matrix can promote the differentiation yield, whilst both the contact and non-contact cultures can generate functional NC-like cells. The generated NC-like cells are highly homogenous regarding the expression of notochordal marker genes. A culture medium containing a cocktail of growth factors (FGF, EGF, VEGF and IGF-1) also supported the notochordal differentiation in the presence of NP matrix. The NC-like cells showed excellent functional differentiation ability to generate NP-like tissue which was rich in aggrecan and collagen type II; and particularly, the proteoglycan to collagen content ratio was as high as 12.5-17.5 which represents a phenotype close to NP rather than hyaline cartilage. Collectively, the present study confirmed the effectiveness and flexibility of using natural NP tissue matrix to direct notochordal differentiation of hiPSCs, and the potential of using the generated NC-like cells for treating IVD degeneration.
format	article
author	Yongxing Liu Mohamed N Rahaman B Sonny Bal
author_facet	Yongxing Liu Mohamed N Rahaman B Sonny Bal
author_sort	Yongxing Liu
title	Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.
title_short	Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.
title_full	Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.
title_fullStr	Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.
title_full_unstemmed	Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.
title_sort	modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/af75ab5a2e624742a2522ac902f5bdfb
work_keys_str_mv	AT yongxingliu modulatingnotochordaldifferentiationofhumaninducedpluripotentstemcellsusingnaturalnucleuspulposustissuematrix AT mohamednrahaman modulatingnotochordaldifferentiationofhumaninducedpluripotentstemcellsusingnaturalnucleuspulposustissuematrix AT bsonnybal modulatingnotochordaldifferentiationofhumaninducedpluripotentstemcellsusingnaturalnucleuspulposustissuematrix
_version_	1718414183098220544

Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.

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