Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion

Single-molecule localisation microscopy is limited by low labeling and detection efficiencies of the molecular probes. Here the authors report a framework to obtain absolute molecular quantities on a true molecular scale; the data reveal a ternary adhesion complex underlying cell-matrix adhesion.

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Detalles Bibliográficos
Autores principales: Lisa S. Fischer, Christoph Klingner, Thomas Schlichthaerle, Maximilian T. Strauss, Ralph Böttcher, Reinhard Fässler, Ralf Jungmann, Carsten Grashoff
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/af7ee6c4a1ba419ab1ee68cefdf933b9
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Sumario:Single-molecule localisation microscopy is limited by low labeling and detection efficiencies of the molecular probes. Here the authors report a framework to obtain absolute molecular quantities on a true molecular scale; the data reveal a ternary adhesion complex underlying cell-matrix adhesion.