Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion

Single-molecule localisation microscopy is limited by low labeling and detection efficiencies of the molecular probes. Here the authors report a framework to obtain absolute molecular quantities on a true molecular scale; the data reveal a ternary adhesion complex underlying cell-matrix adhesion.

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Autores principales:	Lisa S. Fischer, Christoph Klingner, Thomas Schlichthaerle, Maximilian T. Strauss, Ralph Böttcher, Reinhard Fässler, Ralf Jungmann, Carsten Grashoff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/af7ee6c4a1ba419ab1ee68cefdf933b9
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spelling	oai:doaj.org-article:af7ee6c4a1ba419ab1ee68cefdf933b92021-12-02T14:11:08ZQuantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion10.1038/s41467-021-21142-22041-1723https://doaj.org/article/af7ee6c4a1ba419ab1ee68cefdf933b92021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21142-2https://doaj.org/toc/2041-1723Single-molecule localisation microscopy is limited by low labeling and detection efficiencies of the molecular probes. Here the authors report a framework to obtain absolute molecular quantities on a true molecular scale; the data reveal a ternary adhesion complex underlying cell-matrix adhesion.Lisa S. FischerChristoph KlingnerThomas SchlichthaerleMaximilian T. StraussRalph BöttcherReinhard FässlerRalf JungmannCarsten GrashoffNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Lisa S. Fischer Christoph Klingner Thomas Schlichthaerle Maximilian T. Strauss Ralph Böttcher Reinhard Fässler Ralf Jungmann Carsten Grashoff Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
description	Single-molecule localisation microscopy is limited by low labeling and detection efficiencies of the molecular probes. Here the authors report a framework to obtain absolute molecular quantities on a true molecular scale; the data reveal a ternary adhesion complex underlying cell-matrix adhesion.
format	article
author	Lisa S. Fischer Christoph Klingner Thomas Schlichthaerle Maximilian T. Strauss Ralph Böttcher Reinhard Fässler Ralf Jungmann Carsten Grashoff
author_facet	Lisa S. Fischer Christoph Klingner Thomas Schlichthaerle Maximilian T. Strauss Ralph Böttcher Reinhard Fässler Ralf Jungmann Carsten Grashoff
author_sort	Lisa S. Fischer
title	Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
title_short	Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
title_full	Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
title_fullStr	Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
title_full_unstemmed	Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
title_sort	quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/af7ee6c4a1ba419ab1ee68cefdf933b9
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Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion

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