Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method

Abstract Visualization of specific cells in the three-dimensional organ architecture is one of the key steps to develop our knowledge about pathophysiological mechanisms in various organs. In this study, we successfully obtained stereoscopic whole images of the intrauterine murine embryo and placent...

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Autores principales:	Kyosuke Kagami, Yohei Shinmyo, Masanori Ono, Hiroshi Kawasaki, Hiroshi Fujiwara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af8040cb0dd247108fa228496e77ad48
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spelling	oai:doaj.org-article:af8040cb0dd247108fa228496e77ad482021-12-02T12:32:46ZThree-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method10.1038/s41598-017-06549-62045-2322https://doaj.org/article/af8040cb0dd247108fa228496e77ad482017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06549-6https://doaj.org/toc/2045-2322Abstract Visualization of specific cells in the three-dimensional organ architecture is one of the key steps to develop our knowledge about pathophysiological mechanisms in various organs. In this study, we successfully obtained stereoscopic whole images of the intrauterine murine embryo and placenta through the uterus using a modified tissue clearing CUBIC method. By this procedure, we can recognize the three-dimensional relationships among various tissues within the pregnant uterus and analyze free-angle images of cross-sections with single-cell resolution using a computer system. Based on these data, we can select optimal cross-section angles and then produce the corresponding tissue slices that are adequate for further immunohistochemical examination. Furthermore, using transgenic mice, distinct images of an EGFP-positive embryo and the placenta can be obtained, confirming the precise three-dimensional location of invading trophoblasts in the feto-maternal interface in the uterus. These results indicate that this procedure will significantly contribute to analyzing pathophysiological mechanisms in reproductive organs.Kyosuke KagamiYohei ShinmyoMasanori OnoHiroshi KawasakiHiroshi FujiwaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kyosuke Kagami Yohei Shinmyo Masanori Ono Hiroshi Kawasaki Hiroshi Fujiwara Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method
description	Abstract Visualization of specific cells in the three-dimensional organ architecture is one of the key steps to develop our knowledge about pathophysiological mechanisms in various organs. In this study, we successfully obtained stereoscopic whole images of the intrauterine murine embryo and placenta through the uterus using a modified tissue clearing CUBIC method. By this procedure, we can recognize the three-dimensional relationships among various tissues within the pregnant uterus and analyze free-angle images of cross-sections with single-cell resolution using a computer system. Based on these data, we can select optimal cross-section angles and then produce the corresponding tissue slices that are adequate for further immunohistochemical examination. Furthermore, using transgenic mice, distinct images of an EGFP-positive embryo and the placenta can be obtained, confirming the precise three-dimensional location of invading trophoblasts in the feto-maternal interface in the uterus. These results indicate that this procedure will significantly contribute to analyzing pathophysiological mechanisms in reproductive organs.
format	article
author	Kyosuke Kagami Yohei Shinmyo Masanori Ono Hiroshi Kawasaki Hiroshi Fujiwara
author_facet	Kyosuke Kagami Yohei Shinmyo Masanori Ono Hiroshi Kawasaki Hiroshi Fujiwara
author_sort	Kyosuke Kagami
title	Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method
title_short	Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method
title_full	Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method
title_fullStr	Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method
title_full_unstemmed	Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method
title_sort	three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/af8040cb0dd247108fa228496e77ad48
work_keys_str_mv	AT kyosukekagami threedimensionalvisualizationofintrauterineconceptusthroughtheuterinewallbytissueclearingmethod AT yoheishinmyo threedimensionalvisualizationofintrauterineconceptusthroughtheuterinewallbytissueclearingmethod AT masanoriono threedimensionalvisualizationofintrauterineconceptusthroughtheuterinewallbytissueclearingmethod AT hiroshikawasaki threedimensionalvisualizationofintrauterineconceptusthroughtheuterinewallbytissueclearingmethod AT hiroshifujiwara threedimensionalvisualizationofintrauterineconceptusthroughtheuterinewallbytissueclearingmethod
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Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method

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