Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model

Abstract Background Because of the high frequency of chronic edema formation in the current “aged” society, analyses and detailed observation of post-surgical edema are getting more required. Post-surgical examination of the dynamic vasculature including L.V. (Lymphatic Vasculature) to monitor edema...

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Autores principales:	Shinji Kumegawa, Gen Yamada, Daiki Hashimoto, Tsuyoshi Hirashima, Mizuki Kajimoto, Kyoichi Isono, Kota Fujimoto, Kentaro Suzuki, Kazuhisa Uemura, Masatsugu Ema, Shinichi Asamura
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Lymphedema Lymphatic vasculature Tail model Live imaging Transparent sheet 3D reconstruction Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/af8f1e4444c94d0c8ba9c8e7fb2d3947
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spelling	oai:doaj.org-article:af8f1e4444c94d0c8ba9c8e7fb2d39472021-11-14T12:07:25ZDevelopment of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model10.1186/s12575-021-00159-31480-9222https://doaj.org/article/af8f1e4444c94d0c8ba9c8e7fb2d39472021-11-01T00:00:00Zhttps://doi.org/10.1186/s12575-021-00159-3https://doaj.org/toc/1480-9222Abstract Background Because of the high frequency of chronic edema formation in the current “aged” society, analyses and detailed observation of post-surgical edema are getting more required. Post-surgical examination of the dynamic vasculature including L.V. (Lymphatic Vasculature) to monitor edema formation has not been efficiently performed. Hence, procedures for investigating such vasculature are essential. By inserting transparent sheet into the cutaneous layer of mouse tails as a novel surgery model (the T ail E dema by S ilicone sheet mediated T ransparency protocol; TEST), the novel procedures are introduced and analyzed by series of histological analyses including video-based L.V. observation and 3D histological reconstruction of vasculatures in mouse tails. Results The dynamic generation of post-surgical main and fine (neo) L.V. connective structure during the edematous recovery process was visualized by series of studies with a novel surgery model. Snapshot images taken from live binocular image recording for TEST samples suggested the presence of main and elongating fine (neo) L.V. structure. After the ligation of L.V., the enlargement of main L.V. was confirmed. In the case of light sheet fluorescence microscopy (LSFM) observation, such L.V. connections were also suggested by using transparent 3D samples. Finally, the generation of neo blood vessels particularly in the region adjacent to the silicone sheet and the operated boundary region was suggested in 3D reconstruction images. However, direct detection of elongating fine (neo) L.V. was not suitable for analysis by such LSFM and 3D reconstruction procedures. Thus, such methods utilizing fixed tissues are appropriate for general observation for the operated region including of L.V. Conclusions The current surgical procedures and analysis on the post-surgical status are the first case to observe vasculatures in vivo with a transparent sheet. Systematic analyses including the FITC-dextran mediated snap shot images observation suggest the elongation of fine (neo) lymphatic vasculature. Post-surgical analyses including LSFM and 3D histological structural reconstruction, are suitable to reveal the fixed structures of blood and lymphatic vessels formation.Shinji KumegawaGen YamadaDaiki HashimotoTsuyoshi HirashimaMizuki KajimotoKyoichi IsonoKota FujimotoKentaro SuzukiKazuhisa UemuraMasatsugu EmaShinichi AsamuraBMCarticleLymphedemaLymphatic vasculatureTail modelLive imagingTransparent sheet3D reconstructionMedicine (General)R5-920Biology (General)QH301-705.5ENBiological Procedures Online, Vol 23, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lymphedema Lymphatic vasculature Tail model Live imaging Transparent sheet 3D reconstruction Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Lymphedema Lymphatic vasculature Tail model Live imaging Transparent sheet 3D reconstruction Medicine (General) R5-920 Biology (General) QH301-705.5 Shinji Kumegawa Gen Yamada Daiki Hashimoto Tsuyoshi Hirashima Mizuki Kajimoto Kyoichi Isono Kota Fujimoto Kentaro Suzuki Kazuhisa Uemura Masatsugu Ema Shinichi Asamura Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model
description	Abstract Background Because of the high frequency of chronic edema formation in the current “aged” society, analyses and detailed observation of post-surgical edema are getting more required. Post-surgical examination of the dynamic vasculature including L.V. (Lymphatic Vasculature) to monitor edema formation has not been efficiently performed. Hence, procedures for investigating such vasculature are essential. By inserting transparent sheet into the cutaneous layer of mouse tails as a novel surgery model (the T ail E dema by S ilicone sheet mediated T ransparency protocol; TEST), the novel procedures are introduced and analyzed by series of histological analyses including video-based L.V. observation and 3D histological reconstruction of vasculatures in mouse tails. Results The dynamic generation of post-surgical main and fine (neo) L.V. connective structure during the edematous recovery process was visualized by series of studies with a novel surgery model. Snapshot images taken from live binocular image recording for TEST samples suggested the presence of main and elongating fine (neo) L.V. structure. After the ligation of L.V., the enlargement of main L.V. was confirmed. In the case of light sheet fluorescence microscopy (LSFM) observation, such L.V. connections were also suggested by using transparent 3D samples. Finally, the generation of neo blood vessels particularly in the region adjacent to the silicone sheet and the operated boundary region was suggested in 3D reconstruction images. However, direct detection of elongating fine (neo) L.V. was not suitable for analysis by such LSFM and 3D reconstruction procedures. Thus, such methods utilizing fixed tissues are appropriate for general observation for the operated region including of L.V. Conclusions The current surgical procedures and analysis on the post-surgical status are the first case to observe vasculatures in vivo with a transparent sheet. Systematic analyses including the FITC-dextran mediated snap shot images observation suggest the elongation of fine (neo) lymphatic vasculature. Post-surgical analyses including LSFM and 3D histological structural reconstruction, are suitable to reveal the fixed structures of blood and lymphatic vessels formation.
format	article
author	Shinji Kumegawa Gen Yamada Daiki Hashimoto Tsuyoshi Hirashima Mizuki Kajimoto Kyoichi Isono Kota Fujimoto Kentaro Suzuki Kazuhisa Uemura Masatsugu Ema Shinichi Asamura
author_facet	Shinji Kumegawa Gen Yamada Daiki Hashimoto Tsuyoshi Hirashima Mizuki Kajimoto Kyoichi Isono Kota Fujimoto Kentaro Suzuki Kazuhisa Uemura Masatsugu Ema Shinichi Asamura
author_sort	Shinji Kumegawa
title	Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model
title_short	Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model
title_full	Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model
title_fullStr	Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model
title_full_unstemmed	Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model
title_sort	development of surgical and visualization procedures to analyze vasculatures by mouse tail edema model
publisher	BMC
publishDate	2021
url	https://doaj.org/article/af8f1e4444c94d0c8ba9c8e7fb2d3947
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Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model

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