Identification and functional analysis of bacteria in sclerotia of Cordyceps militaris

Background Cordyceps militaris is a fungus that parasitizes insects. Compounds from C. militaris are valuable in medicine and functional food. There are many kinds of bacteria in the natural sclerotia of C. militaris. However, the community structure of microorganisms in samples from different place...

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Autores principales:	Li Luo, Jiaxi Zhou, Zhongshun Xu, Jingqiang Guan, Yingming Gao, Xiao Zou
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Cordyceps militaris Microbial community Co-culture Functional analysis Medicine R
Acceso en línea:	https://doaj.org/article/af94be230b2a40108997da3ade983786
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spelling	oai:doaj.org-article:af94be230b2a40108997da3ade9837862021-11-27T15:05:06ZIdentification and functional analysis of bacteria in sclerotia of Cordyceps militaris10.7717/peerj.125112167-8359https://doaj.org/article/af94be230b2a40108997da3ade9837862021-11-01T00:00:00Zhttps://peerj.com/articles/12511.pdfhttps://peerj.com/articles/12511/https://doaj.org/toc/2167-8359Background Cordyceps militaris is a fungus that parasitizes insects. Compounds from C. militaris are valuable in medicine and functional food. There are many kinds of bacteria in the natural sclerotia of C. militaris. However, the community structure of microorganisms in samples from different places may be different, and their corresponding ecological functions require experimental verification. Methods We used high-throughput sequencing technology to analyze bacterial 16S rRNA gene sequences in sclerotia of three samples of C. militaris from Liaoning Province, China. We isolated, identified and verified the function of culturable bacterial strains from the sclerotia. Results Pseudomonas, Pedobacter, Sphingobacterium, and Serratia were the dominant bacterial genera in the sclerotia. And function prediction showed that Pseudomonas and Pedobacter could be heterotrophic, Sphingobacterium could decompose urea, and Serratia could reduce nitrate. Two strains of bacteria isolated from the sclerotia of C. militaris, N-2 and N-26, were identified as Stenotrophomonas maltophilia and Pseudomonas baetica, respectively, based on culture and biochemical characteristics. When these isolated strains were co-cultured with C. militaris, the mycelium biomass and mycelium pellet diameter decreased, and the content of extracellular polysaccharide increased. Strain N-26 decreased the cordycepin content in C. militaris. Conclusions Bacteria in sclerotia have an important effect on the growth of C. militaris and the production of its metabolites.Li LuoJiaxi ZhouZhongshun XuJingqiang GuanYingming GaoXiao ZouPeerJ Inc.articleCordyceps militarisMicrobial communityCo-cultureFunctional analysisMedicineRENPeerJ, Vol 9, p e12511 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cordyceps militaris Microbial community Co-culture Functional analysis Medicine R
spellingShingle	Cordyceps militaris Microbial community Co-culture Functional analysis Medicine R Li Luo Jiaxi Zhou Zhongshun Xu Jingqiang Guan Yingming Gao Xiao Zou Identification and functional analysis of bacteria in sclerotia of Cordyceps militaris
description	Background Cordyceps militaris is a fungus that parasitizes insects. Compounds from C. militaris are valuable in medicine and functional food. There are many kinds of bacteria in the natural sclerotia of C. militaris. However, the community structure of microorganisms in samples from different places may be different, and their corresponding ecological functions require experimental verification. Methods We used high-throughput sequencing technology to analyze bacterial 16S rRNA gene sequences in sclerotia of three samples of C. militaris from Liaoning Province, China. We isolated, identified and verified the function of culturable bacterial strains from the sclerotia. Results Pseudomonas, Pedobacter, Sphingobacterium, and Serratia were the dominant bacterial genera in the sclerotia. And function prediction showed that Pseudomonas and Pedobacter could be heterotrophic, Sphingobacterium could decompose urea, and Serratia could reduce nitrate. Two strains of bacteria isolated from the sclerotia of C. militaris, N-2 and N-26, were identified as Stenotrophomonas maltophilia and Pseudomonas baetica, respectively, based on culture and biochemical characteristics. When these isolated strains were co-cultured with C. militaris, the mycelium biomass and mycelium pellet diameter decreased, and the content of extracellular polysaccharide increased. Strain N-26 decreased the cordycepin content in C. militaris. Conclusions Bacteria in sclerotia have an important effect on the growth of C. militaris and the production of its metabolites.
format	article
author	Li Luo Jiaxi Zhou Zhongshun Xu Jingqiang Guan Yingming Gao Xiao Zou
author_facet	Li Luo Jiaxi Zhou Zhongshun Xu Jingqiang Guan Yingming Gao Xiao Zou
author_sort	Li Luo
title	Identification and functional analysis of bacteria in sclerotia of Cordyceps militaris
title_short	Identification and functional analysis of bacteria in sclerotia of Cordyceps militaris
title_full	Identification and functional analysis of bacteria in sclerotia of Cordyceps militaris
title_fullStr	Identification and functional analysis of bacteria in sclerotia of Cordyceps militaris
title_full_unstemmed	Identification and functional analysis of bacteria in sclerotia of Cordyceps militaris
title_sort	identification and functional analysis of bacteria in sclerotia of cordyceps militaris
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/af94be230b2a40108997da3ade983786
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Identification and functional analysis of bacteria in sclerotia of Cordyceps militaris

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