Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea

Abstract. Jafari H, Honari H, Zargan J, Jahromi ST. 2019. Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea. Biodiversitas 20: 1228-1232. The present study investigated the hemolytic capacity of the crude venom extrac...

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Autores principales:	HOSSEIN JAFARI, HOSSEIN HONARI, JAMIL ZARGAN, SAEID TAMADONI JAHROMI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	venom, nematocysts, hemolytic assay,rhopilema sp, Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/af98cf388b8d42a09dc8b7a768c7395d
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spelling	oai:doaj.org-article:af98cf388b8d42a09dc8b7a768c7395d2021-11-21T21:40:38ZIdentification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea1412-033X2085-472210.13057/biodiv/d200440https://doaj.org/article/af98cf388b8d42a09dc8b7a768c7395d2019-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3075https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Jafari H, Honari H, Zargan J, Jahromi ST. 2019. Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea. Biodiversitas 20: 1228-1232. The present study investigated the hemolytic capacity of the crude venom extracted from isolated nematocysts of Rhopilema sp. Scyphozoa: Rhizostomeae. Nematocyst was used at various concentrations to evaluate the hemolytic activity by using the nematocysts of human, mice, and sheep. Mean concentration-dependent hemolysis could be observed from 200 µg/mL of protein equivalents or higher with variable potencies in different species. The crude venom was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE. Molecular weight with 3 clear bands including 45, 65 and 95 kDa appeared to be the major protein components of the venom. The results of our experiments indicated that venom of Rhopilema sp. induces hemolysis in the studied species and determined that the increase in the amount of toxin has a positive correlation with the increase of cell lysis. This study showed that the venom of Rhopilema sp. may have many biologically active principles, which need further studies in the future.HOSSEIN JAFARIHOSSEIN HONARIJAMIL ZARGANSAEID TAMADONI JAHROMIMBI & UNS Soloarticlevenom, nematocysts, hemolytic assay,rhopilema sp,Biology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 4, Pp 1228-1232 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	venom, nematocysts, hemolytic assay,rhopilema sp, Biology (General) QH301-705.5
spellingShingle	venom, nematocysts, hemolytic assay,rhopilema sp, Biology (General) QH301-705.5 HOSSEIN JAFARI HOSSEIN HONARI JAMIL ZARGAN SAEID TAMADONI JAHROMI Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea
description	Abstract. Jafari H, Honari H, Zargan J, Jahromi ST. 2019. Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea. Biodiversitas 20: 1228-1232. The present study investigated the hemolytic capacity of the crude venom extracted from isolated nematocysts of Rhopilema sp. Scyphozoa: Rhizostomeae. Nematocyst was used at various concentrations to evaluate the hemolytic activity by using the nematocysts of human, mice, and sheep. Mean concentration-dependent hemolysis could be observed from 200 µg/mL of protein equivalents or higher with variable potencies in different species. The crude venom was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE. Molecular weight with 3 clear bands including 45, 65 and 95 kDa appeared to be the major protein components of the venom. The results of our experiments indicated that venom of Rhopilema sp. induces hemolysis in the studied species and determined that the increase in the amount of toxin has a positive correlation with the increase of cell lysis. This study showed that the venom of Rhopilema sp. may have many biologically active principles, which need further studies in the future.
format	article
author	HOSSEIN JAFARI HOSSEIN HONARI JAMIL ZARGAN SAEID TAMADONI JAHROMI
author_facet	HOSSEIN JAFARI HOSSEIN HONARI JAMIL ZARGAN SAEID TAMADONI JAHROMI
author_sort	HOSSEIN JAFARI
title	Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea
title_short	Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea
title_full	Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea
title_fullStr	Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea
title_full_unstemmed	Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea
title_sort	identification and hemolytic activity of jellyfish (rhopilema sp., scyphozoa: rhizostomeae) venom from the persian gulf and oman sea
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/af98cf388b8d42a09dc8b7a768c7395d
work_keys_str_mv	AT hosseinjafari identificationandhemolyticactivityofjellyfishrhopilemaspscyphozoarhizostomeaevenomfromthepersiangulfandomansea AT hosseinhonari identificationandhemolyticactivityofjellyfishrhopilemaspscyphozoarhizostomeaevenomfromthepersiangulfandomansea AT jamilzargan identificationandhemolyticactivityofjellyfishrhopilemaspscyphozoarhizostomeaevenomfromthepersiangulfandomansea AT saeidtamadonijahromi identificationandhemolyticactivityofjellyfishrhopilemaspscyphozoarhizostomeaevenomfromthepersiangulfandomansea
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Identification and hemolytic activity of jellyfish (Rhopilema sp., Scyphozoa: Rhizostomeae) venom from the Persian Gulf and Oman Sea

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