Wound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies

<i>Gnathonemus</i><i>petersii</i> (F. Mormyridae) commonly known as Peters’ elephant-nose fish is a freshwater elephant fish native to West and Central African rivers. The present research aimed at metabolic profiling of its derived crude oil via GC-MS analysis. In addition,...

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Autores principales:	Faisal Alsenani, Ahmed M. Ashour, Mubarak A. Alzubaidi, Ahmed F. Azmy, Mona H. Hetta, Dalia H. Abu-Baih, Mahmoud A. Elrehany, Ahmed Zayed, Ahmed M. Sayed, Usama Ramadan Abdelmohsen, Abeer H. Elmaidomy
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Peters’ elephant-nose fish GC-MS fatty acids COX molecular modeling molecular dynamic simulation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/af9d8fb12c6541c6809c246efaeb891c
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id	oai:doaj.org-article:af9d8fb12c6541c6809c246efaeb891c
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spelling	oai:doaj.org-article:af9d8fb12c6541c6809c246efaeb891c2021-11-25T18:12:46ZWound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies10.3390/md191106051660-3397https://doaj.org/article/af9d8fb12c6541c6809c246efaeb891c2021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/605https://doaj.org/toc/1660-3397<i>Gnathonemus</i><i>petersii</i> (F. Mormyridae) commonly known as Peters’ elephant-nose fish is a freshwater elephant fish native to West and Central African rivers. The present research aimed at metabolic profiling of its derived crude oil via GC-MS analysis. In addition, wound healing aptitude in adult male New Zealand Dutch strain albino rabbits along with isolated bioactive compounds in comparison with a commercial product (Mebo<sup>®</sup>). The molecular mechanism was studied through a number of in vitro investigations, i.e., radical scavenging and inhibition of COX enzymes, in addition to in silico molecular docking study. The results revealed a total of 35 identified (71.11%) compounds in the fish oil, belonging to fatty acids (59.57%), sterols (6.11%), and alkanes (5.43%). Phytochemical investigation of the crude oil afforded isolation of six compounds <b>1–6</b>. Moreover, the crude oil showed significant in vitro hydrogen peroxide and superoxide radical scavenging activities. Furthermore, the crude oil along with one of its major components (compound <b>4</b>) exhibited selective inhibitory activity towards COX-2 with IC<sub>50</sub> values of 15.27 and 2.41 µM, respectively. Topical application of the crude oil on excision wounds showed a significant (<i>p</i> < 0.05) increase in the wound healing rate in comparison to the untreated and Mebo<sup>®</sup>-treated groups, where fish oil increased the TGF-β1 expression, down-regulated TNF-<i>α</i>, and IL-1β. Accordingly, Peters’ elephant-nose fish oil may be a potential alternative medication helping wound healing owing to its antioxidant and anti-inflammatory activities.Faisal AlsenaniAhmed M. AshourMubarak A. AlzubaidiAhmed F. AzmyMona H. HettaDalia H. Abu-BaihMahmoud A. ElrehanyAhmed ZayedAhmed M. SayedUsama Ramadan AbdelmohsenAbeer H. ElmaidomyMDPI AGarticlePeters’ elephant-nose fishGC-MSfatty acidsCOXmolecular modelingmolecular dynamic simulationBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 605, p 605 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Peters’ elephant-nose fish GC-MS fatty acids COX molecular modeling molecular dynamic simulation Biology (General) QH301-705.5
spellingShingle	Peters’ elephant-nose fish GC-MS fatty acids COX molecular modeling molecular dynamic simulation Biology (General) QH301-705.5 Faisal Alsenani Ahmed M. Ashour Mubarak A. Alzubaidi Ahmed F. Azmy Mona H. Hetta Dalia H. Abu-Baih Mahmoud A. Elrehany Ahmed Zayed Ahmed M. Sayed Usama Ramadan Abdelmohsen Abeer H. Elmaidomy Wound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies
description	<i>Gnathonemus</i><i>petersii</i> (F. Mormyridae) commonly known as Peters’ elephant-nose fish is a freshwater elephant fish native to West and Central African rivers. The present research aimed at metabolic profiling of its derived crude oil via GC-MS analysis. In addition, wound healing aptitude in adult male New Zealand Dutch strain albino rabbits along with isolated bioactive compounds in comparison with a commercial product (Mebo<sup>®</sup>). The molecular mechanism was studied through a number of in vitro investigations, i.e., radical scavenging and inhibition of COX enzymes, in addition to in silico molecular docking study. The results revealed a total of 35 identified (71.11%) compounds in the fish oil, belonging to fatty acids (59.57%), sterols (6.11%), and alkanes (5.43%). Phytochemical investigation of the crude oil afforded isolation of six compounds <b>1–6</b>. Moreover, the crude oil showed significant in vitro hydrogen peroxide and superoxide radical scavenging activities. Furthermore, the crude oil along with one of its major components (compound <b>4</b>) exhibited selective inhibitory activity towards COX-2 with IC<sub>50</sub> values of 15.27 and 2.41 µM, respectively. Topical application of the crude oil on excision wounds showed a significant (<i>p</i> < 0.05) increase in the wound healing rate in comparison to the untreated and Mebo<sup>®</sup>-treated groups, where fish oil increased the TGF-β1 expression, down-regulated TNF-<i>α</i>, and IL-1β. Accordingly, Peters’ elephant-nose fish oil may be a potential alternative medication helping wound healing owing to its antioxidant and anti-inflammatory activities.
format	article
author	Faisal Alsenani Ahmed M. Ashour Mubarak A. Alzubaidi Ahmed F. Azmy Mona H. Hetta Dalia H. Abu-Baih Mahmoud A. Elrehany Ahmed Zayed Ahmed M. Sayed Usama Ramadan Abdelmohsen Abeer H. Elmaidomy
author_facet	Faisal Alsenani Ahmed M. Ashour Mubarak A. Alzubaidi Ahmed F. Azmy Mona H. Hetta Dalia H. Abu-Baih Mahmoud A. Elrehany Ahmed Zayed Ahmed M. Sayed Usama Ramadan Abdelmohsen Abeer H. Elmaidomy
author_sort	Faisal Alsenani
title	Wound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies
title_short	Wound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies
title_full	Wound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies
title_fullStr	Wound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies
title_full_unstemmed	Wound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies
title_sort	wound healing metabolites from peters’ elephant-nose fish oil: an in vivo investigation supported by in vitro and in silico studies
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/af9d8fb12c6541c6809c246efaeb891c
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Wound Healing Metabolites from Peters’ Elephant-Nose Fish Oil: An In Vivo Investigation Supported by In Vitro and In Silico Studies

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