Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.

Extremely low birth weight (ELBW) infants have high morbidity and mortality, frequently due to invasive infections from bacteria, fungi, and viruses. The microbial communities present in the gastrointestinal tracts of preterm infants may serve as a reservoir for invasive organisms and remain poorly...

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Autores principales:	Mariam Susan LaTuga, Joseph Christopher Ellis, Charles Michael Cotton, Ronald N Goldberg, James L Wynn, Robert B Jackson, Patrick C Seed
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/afa64f0b062d49389895a241761d995b
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spelling	oai:doaj.org-article:afa64f0b062d49389895a241761d995b2021-11-18T07:32:46ZBeyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.1932-620310.1371/journal.pone.0027858https://doaj.org/article/afa64f0b062d49389895a241761d995b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22174751/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Extremely low birth weight (ELBW) infants have high morbidity and mortality, frequently due to invasive infections from bacteria, fungi, and viruses. The microbial communities present in the gastrointestinal tracts of preterm infants may serve as a reservoir for invasive organisms and remain poorly characterized. We used deep pyrosequencing to examine the gut-associated microbiome of 11 ELBW infants in the first postnatal month, with a first time determination of the eukaryote microbiota such as fungi and nematodes, including bacteria and viruses that have not been previously described. Among the fungi observed, Candida sp. and Clavispora sp. dominated the sequences, but a range of environmental molds were also observed. Surprisingly, seventy-one percent of the infant fecal samples tested contained ribosomal sequences corresponding to the parasitic organism Trichinella. Ribosomal DNA sequences for the roundworm symbiont Xenorhabdus accompanied these sequences in the infant with the greatest proportion of Trichinella sequences. When examining ribosomal DNA sequences in aggregate, Enterobacteriales, Pseudomonas, Staphylococcus, and Enterococcus were the most abundant bacterial taxa in a low diversity bacterial community (mean Shannon-Weaver Index of 1.02 ± 0.69), with relatively little change within individual infants through time. To supplement the ribosomal sequence data, shotgun sequencing was performed on DNA from multiple displacement amplification (MDA) of total fecal genomic DNA from two infants. In addition to the organisms mentioned previously, the metagenome also revealed sequences for gram positive and gram negative bacteriophages, as well as human adenovirus C. Together, these data reveal surprising eukaryotic and viral microbial diversity in ELBW enteric microbiota dominated bytypes of bacteria known to cause invasive disease in these infants.Mariam Susan LaTugaJoseph Christopher EllisCharles Michael CottonRonald N GoldbergJames L WynnRobert B JacksonPatrick C SeedPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e27858 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariam Susan LaTuga Joseph Christopher Ellis Charles Michael Cotton Ronald N Goldberg James L Wynn Robert B Jackson Patrick C Seed Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.
description	Extremely low birth weight (ELBW) infants have high morbidity and mortality, frequently due to invasive infections from bacteria, fungi, and viruses. The microbial communities present in the gastrointestinal tracts of preterm infants may serve as a reservoir for invasive organisms and remain poorly characterized. We used deep pyrosequencing to examine the gut-associated microbiome of 11 ELBW infants in the first postnatal month, with a first time determination of the eukaryote microbiota such as fungi and nematodes, including bacteria and viruses that have not been previously described. Among the fungi observed, Candida sp. and Clavispora sp. dominated the sequences, but a range of environmental molds were also observed. Surprisingly, seventy-one percent of the infant fecal samples tested contained ribosomal sequences corresponding to the parasitic organism Trichinella. Ribosomal DNA sequences for the roundworm symbiont Xenorhabdus accompanied these sequences in the infant with the greatest proportion of Trichinella sequences. When examining ribosomal DNA sequences in aggregate, Enterobacteriales, Pseudomonas, Staphylococcus, and Enterococcus were the most abundant bacterial taxa in a low diversity bacterial community (mean Shannon-Weaver Index of 1.02 ± 0.69), with relatively little change within individual infants through time. To supplement the ribosomal sequence data, shotgun sequencing was performed on DNA from multiple displacement amplification (MDA) of total fecal genomic DNA from two infants. In addition to the organisms mentioned previously, the metagenome also revealed sequences for gram positive and gram negative bacteriophages, as well as human adenovirus C. Together, these data reveal surprising eukaryotic and viral microbial diversity in ELBW enteric microbiota dominated bytypes of bacteria known to cause invasive disease in these infants.
format	article
author	Mariam Susan LaTuga Joseph Christopher Ellis Charles Michael Cotton Ronald N Goldberg James L Wynn Robert B Jackson Patrick C Seed
author_facet	Mariam Susan LaTuga Joseph Christopher Ellis Charles Michael Cotton Ronald N Goldberg James L Wynn Robert B Jackson Patrick C Seed
author_sort	Mariam Susan LaTuga
title	Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.
title_short	Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.
title_full	Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.
title_fullStr	Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.
title_full_unstemmed	Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.
title_sort	beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/afa64f0b062d49389895a241761d995b
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Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.

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