Proteotyping to establish gene origin within reassortant influenza viruses.

The application of a rapid and direct proteotyping approach with which to identify the gene origin of viral antigens in a reassortant influenza strain is demonstrated. The reassortant strain, constructed for a vaccine against type A 2009 H1N1 pandemic influenza, contains genes derived from a wild-ty...

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Autores principales:	Ji-won Ha, Alexander B Schwahn, Kevin M Downard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/afb954fd900344d4b0ac7625aacb00d3
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spelling	oai:doaj.org-article:afb954fd900344d4b0ac7625aacb00d32021-11-18T06:59:22ZProteotyping to establish gene origin within reassortant influenza viruses.1932-620310.1371/journal.pone.0015771https://doaj.org/article/afb954fd900344d4b0ac7625aacb00d32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21305059/?tool=EBIhttps://doaj.org/toc/1932-6203The application of a rapid and direct proteotyping approach with which to identify the gene origin of viral antigens in a reassortant influenza strain is demonstrated. The reassortant strain, constructed for a vaccine against type A 2009 H1N1 pandemic influenza, contains genes derived from a wild-type pandemic strain (A/California/7/2009) and an egg adapted high-growth strain (denoted NYMC X-157) derived from an earlier A/Puerto Rico/8/34 strain. The proteotyping approach employs modern proteomics methods and high resolution mass spectrometry to correctly establish that the genes of the surface antigens, hemagglutinin and neuraminidase, are derived from the A/California/7/2009 strain while those for nucleoprotein and matrix protein M1 antigens are derived from the NYMC X-157 strain. This is achieved for both gel-separated antigens and those from a whole vaccine digest. Furthermore, signature peptides detected in the mass spectra of the digested antigens enable the engineered reassortant strain to be identified as a type A virus of the H1N1 subtype in accord with earlier studies. The results demonstrate that proteotyping approach provides a more direct and rapid approach over RT-PCR with which to characterize reassortant strains of the influenza virus at the molecular protein level. Given that these strains pose the greatest risk to human and animal health and have been responsible for all human pandemics of the 20th and 21st centuries, there is a vital need for the origins and evolutionary history of these strains to be rapidly established.Ji-won HaAlexander B SchwahnKevin M DownardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e15771 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ji-won Ha Alexander B Schwahn Kevin M Downard Proteotyping to establish gene origin within reassortant influenza viruses.
description	The application of a rapid and direct proteotyping approach with which to identify the gene origin of viral antigens in a reassortant influenza strain is demonstrated. The reassortant strain, constructed for a vaccine against type A 2009 H1N1 pandemic influenza, contains genes derived from a wild-type pandemic strain (A/California/7/2009) and an egg adapted high-growth strain (denoted NYMC X-157) derived from an earlier A/Puerto Rico/8/34 strain. The proteotyping approach employs modern proteomics methods and high resolution mass spectrometry to correctly establish that the genes of the surface antigens, hemagglutinin and neuraminidase, are derived from the A/California/7/2009 strain while those for nucleoprotein and matrix protein M1 antigens are derived from the NYMC X-157 strain. This is achieved for both gel-separated antigens and those from a whole vaccine digest. Furthermore, signature peptides detected in the mass spectra of the digested antigens enable the engineered reassortant strain to be identified as a type A virus of the H1N1 subtype in accord with earlier studies. The results demonstrate that proteotyping approach provides a more direct and rapid approach over RT-PCR with which to characterize reassortant strains of the influenza virus at the molecular protein level. Given that these strains pose the greatest risk to human and animal health and have been responsible for all human pandemics of the 20th and 21st centuries, there is a vital need for the origins and evolutionary history of these strains to be rapidly established.
format	article
author	Ji-won Ha Alexander B Schwahn Kevin M Downard
author_facet	Ji-won Ha Alexander B Schwahn Kevin M Downard
author_sort	Ji-won Ha
title	Proteotyping to establish gene origin within reassortant influenza viruses.
title_short	Proteotyping to establish gene origin within reassortant influenza viruses.
title_full	Proteotyping to establish gene origin within reassortant influenza viruses.
title_fullStr	Proteotyping to establish gene origin within reassortant influenza viruses.
title_full_unstemmed	Proteotyping to establish gene origin within reassortant influenza viruses.
title_sort	proteotyping to establish gene origin within reassortant influenza viruses.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/afb954fd900344d4b0ac7625aacb00d3
work_keys_str_mv	AT jiwonha proteotypingtoestablishgeneoriginwithinreassortantinfluenzaviruses AT alexanderbschwahn proteotypingtoestablishgeneoriginwithinreassortantinfluenzaviruses AT kevinmdownard proteotypingtoestablishgeneoriginwithinreassortantinfluenzaviruses
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Proteotyping to establish gene origin within reassortant influenza viruses.

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