Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells

BACKGROUND AND OBJECTIVE: Silver nanoparticles have antibacterial and anticancer activity. But the organic solvents used to produce these nanoparticles are toxic and can have devastating environmental effects. Therefore, there is a strong desire to use healthy methods for the synthesis of silver nan...

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Autores principales:	Sh Nedaie, H Akbari, H Yaghoubi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	silver nanoparticles truffle fungus(tuber spp.) bio-synthesis mtt mcf-7 cell line Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/afbed82ff96a425e9045f55237d301b6
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spelling	oai:doaj.org-article:afbed82ff96a425e9045f55237d301b62021-11-10T08:30:36ZCytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells1561-41072251-7170https://doaj.org/article/afbed82ff96a425e9045f55237d301b62019-12-01T00:00:00Zhttp://jbums.org/article-1-8140-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Silver nanoparticles have antibacterial and anticancer activity. But the organic solvents used to produce these nanoparticles are toxic and can have devastating environmental effects. Therefore, there is a strong desire to use healthy methods for the synthesis of silver nanoparticles. Therefore, this study was performed to synthesize silver nanoparticles biologically from aqueous extract of Truffle and to study their biological effects on cancer cells. METHODS: In this experimental study, to synthesize silver nanoparticles from aqueous extract of Truffle, 10 ml of fungus extract was added to 90 ml of 1 ml silver nitrate solution and incubated for 72 hours at room temperature. The physicochemical properties of the nanoparticles synthesized by XRD, FESEM and TEM were analyzed. Effects of Extract and Synthesized Nanoparticles were studied by MTT at concentrations of 0.025, 0.25, 0.25, 0.5 and 1 mg / ml on MCF-7 Cancer Cells at 72, 48, and 24 hours. FINDINGS: The size of the nanoparticles was between 19 and 35 nm and their shape was mainly spherical. In cytotoxicity assay using MTT assay IC50 calculated for silver nanoparticles and extract in MCF-7 cells showed that silver nanoparticles had more cytotoxic effects than extract. The calculated IC50 for extracts and nanoparticles at 24, 48 and 72 hours were 0.73, 0.8, and 0.64 mg / ml, respectively, and for the nanoparticles were 0.6, 0.5, and 0.48, respectively (p <0.05). CONCLUSION: The silver nanoparticles produced by bio-synthesis method have a higher cytotoxicity than the truffle fungus (Tuber spp.) extract.Sh NedaieH AkbariH YaghoubiBabol University of Medical Sciencesarticlesilver nanoparticlestruffle fungus(tuber spp.)bio-synthesismttmcf-7 cell lineMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 242-248 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	silver nanoparticles truffle fungus(tuber spp.) bio-synthesis mtt mcf-7 cell line Medicine R Medicine (General) R5-920
spellingShingle	silver nanoparticles truffle fungus(tuber spp.) bio-synthesis mtt mcf-7 cell line Medicine R Medicine (General) R5-920 Sh Nedaie H Akbari H Yaghoubi Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells
description	BACKGROUND AND OBJECTIVE: Silver nanoparticles have antibacterial and anticancer activity. But the organic solvents used to produce these nanoparticles are toxic and can have devastating environmental effects. Therefore, there is a strong desire to use healthy methods for the synthesis of silver nanoparticles. Therefore, this study was performed to synthesize silver nanoparticles biologically from aqueous extract of Truffle and to study their biological effects on cancer cells. METHODS: In this experimental study, to synthesize silver nanoparticles from aqueous extract of Truffle, 10 ml of fungus extract was added to 90 ml of 1 ml silver nitrate solution and incubated for 72 hours at room temperature. The physicochemical properties of the nanoparticles synthesized by XRD, FESEM and TEM were analyzed. Effects of Extract and Synthesized Nanoparticles were studied by MTT at concentrations of 0.025, 0.25, 0.25, 0.5 and 1 mg / ml on MCF-7 Cancer Cells at 72, 48, and 24 hours. FINDINGS: The size of the nanoparticles was between 19 and 35 nm and their shape was mainly spherical. In cytotoxicity assay using MTT assay IC50 calculated for silver nanoparticles and extract in MCF-7 cells showed that silver nanoparticles had more cytotoxic effects than extract. The calculated IC50 for extracts and nanoparticles at 24, 48 and 72 hours were 0.73, 0.8, and 0.64 mg / ml, respectively, and for the nanoparticles were 0.6, 0.5, and 0.48, respectively (p <0.05). CONCLUSION: The silver nanoparticles produced by bio-synthesis method have a higher cytotoxicity than the truffle fungus (Tuber spp.) extract.
format	article
author	Sh Nedaie H Akbari H Yaghoubi
author_facet	Sh Nedaie H Akbari H Yaghoubi
author_sort	Sh Nedaie
title	Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells
title_short	Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells
title_full	Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells
title_fullStr	Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells
title_full_unstemmed	Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells
title_sort	cytotoxicity effects of green synthesized silver nanoparticles by using the extract of tuber spp. on breast cancer (mcf-7) cells
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/afbed82ff96a425e9045f55237d301b6
work_keys_str_mv	AT shnedaie cytotoxicityeffectsofgreensynthesizedsilvernanoparticlesbyusingtheextractoftuberspponbreastcancermcf7cells AT hakbari cytotoxicityeffectsofgreensynthesizedsilvernanoparticlesbyusingtheextractoftuberspponbreastcancermcf7cells AT hyaghoubi cytotoxicityeffectsofgreensynthesizedsilvernanoparticlesbyusingtheextractoftuberspponbreastcancermcf7cells
_version_	1718440408636194816

Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells

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