UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis

Skin aging is the result of superimposed intrinsic (individual) and extrinsic (e.g., UV exposure or nutrition) aging. Previous works have reported a relationship between UV irradiation and glycation in the aging process, leading, for example, to modified radical species production and the appearance...

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Autores principales:	Hervé Pageon, Hélène Zucchi, Sylvie Ricois, Philippe Bastien, Daniel Asselineau
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/afc6add94e0c441ca27ea13e0842e788
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spelling	oai:doaj.org-article:afc6add94e0c441ca27ea13e0842e7882021-11-08T02:36:21ZUVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis2090-221210.1155/2021/6647773https://doaj.org/article/afc6add94e0c441ca27ea13e0842e7882021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/6647773https://doaj.org/toc/2090-2212Skin aging is the result of superimposed intrinsic (individual) and extrinsic (e.g., UV exposure or nutrition) aging. Previous works have reported a relationship between UV irradiation and glycation in the aging process, leading, for example, to modified radical species production and the appearance of AGEs (advanced glycosylation end products) in increasing quantities, particularly glycoxidation products like pentosidine. In addition, the colocalization of AGEs and elastosis has also been observed. We first investigated the combination of the glycation reaction and UVA effects on a reconstructed skin model to explain their cumulative biological effect. We found that UVA exposure combined with glycation had the ability to intensify the response for specific markers: for example, MMP1 or MMP3 mRNA, proteases involved in extracellular matrix degradation, or proinflammatory cytokine, IL1α, protein expression. Moreover, the association of glycation and UVA irradiation is believed to promote an environment that favors the onset of an elastotic-like phenomenon: mRNA coding for elastin, elastase, and tropoelastin expression is increased. Secondly, because the damaging effects of UV radiation in vivo might be more detrimental in aged skin than in young skin due to increased accumulation of pentosidine and the exacerbation of alterations related to chronological aging, we studied the biological effect of soluble pentosidine in fibroblasts grown in monolayers. We found that pentosidine induced upregulation of CXCL2, IL8, and MMP12 mRNA expression (inflammatory and elastotic markers, respectively). Tropoelastin protein expression (elastin precursor) was also increased. In conclusion, fibroblasts in monolayers cultured with soluble pentosidine and tridimensional in vitro skin constructs exposed to the combination of AGEs and UVA promote an inflammatory state and an alteration of the dermal compartment in relation to an elastosis-like environment.Hervé PageonHélène ZucchiSylvie RicoisPhilippe BastienDaniel AsselineauHindawi LimitedarticleGeriatricsRC952-954.6ENJournal of Aging Research, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Geriatrics RC952-954.6
spellingShingle	Geriatrics RC952-954.6 Hervé Pageon Hélène Zucchi Sylvie Ricois Philippe Bastien Daniel Asselineau UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis
description	Skin aging is the result of superimposed intrinsic (individual) and extrinsic (e.g., UV exposure or nutrition) aging. Previous works have reported a relationship between UV irradiation and glycation in the aging process, leading, for example, to modified radical species production and the appearance of AGEs (advanced glycosylation end products) in increasing quantities, particularly glycoxidation products like pentosidine. In addition, the colocalization of AGEs and elastosis has also been observed. We first investigated the combination of the glycation reaction and UVA effects on a reconstructed skin model to explain their cumulative biological effect. We found that UVA exposure combined with glycation had the ability to intensify the response for specific markers: for example, MMP1 or MMP3 mRNA, proteases involved in extracellular matrix degradation, or proinflammatory cytokine, IL1α, protein expression. Moreover, the association of glycation and UVA irradiation is believed to promote an environment that favors the onset of an elastotic-like phenomenon: mRNA coding for elastin, elastase, and tropoelastin expression is increased. Secondly, because the damaging effects of UV radiation in vivo might be more detrimental in aged skin than in young skin due to increased accumulation of pentosidine and the exacerbation of alterations related to chronological aging, we studied the biological effect of soluble pentosidine in fibroblasts grown in monolayers. We found that pentosidine induced upregulation of CXCL2, IL8, and MMP12 mRNA expression (inflammatory and elastotic markers, respectively). Tropoelastin protein expression (elastin precursor) was also increased. In conclusion, fibroblasts in monolayers cultured with soluble pentosidine and tridimensional in vitro skin constructs exposed to the combination of AGEs and UVA promote an inflammatory state and an alteration of the dermal compartment in relation to an elastosis-like environment.
format	article
author	Hervé Pageon Hélène Zucchi Sylvie Ricois Philippe Bastien Daniel Asselineau
author_facet	Hervé Pageon Hélène Zucchi Sylvie Ricois Philippe Bastien Daniel Asselineau
author_sort	Hervé Pageon
title	UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis
title_short	UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis
title_full	UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis
title_fullStr	UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis
title_full_unstemmed	UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis
title_sort	uva exposure combined with glycation of the dermis are two catalysts for skin aging and promotes a favorable environment to the appearance of elastosis
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/afc6add94e0c441ca27ea13e0842e788
work_keys_str_mv	AT hervepageon uvaexposurecombinedwithglycationofthedermisaretwocatalystsforskinagingandpromotesafavorableenvironmenttotheappearanceofelastosis AT helenezucchi uvaexposurecombinedwithglycationofthedermisaretwocatalystsforskinagingandpromotesafavorableenvironmenttotheappearanceofelastosis AT sylviericois uvaexposurecombinedwithglycationofthedermisaretwocatalystsforskinagingandpromotesafavorableenvironmenttotheappearanceofelastosis AT philippebastien uvaexposurecombinedwithglycationofthedermisaretwocatalystsforskinagingandpromotesafavorableenvironmenttotheappearanceofelastosis AT danielasselineau uvaexposurecombinedwithglycationofthedermisaretwocatalystsforskinagingandpromotesafavorableenvironmenttotheappearanceofelastosis
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UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis

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