Search for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.

<h4>Background</h4>Preeclampsia and coronary-artery disease share risk factors, suggesting common pathophysiological mechanisms. CX3CR1/CX3CL1 mediates leukocyte migration and adhesion and has been implicated in the pathophysiology of several inflammatory diseases. M280/I249 variants of...

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Autores principales:	Alain Stepanian, Soraya Benchenni, Tiphaine Beillat-Lucas, Sophie Omnes, Fannie Defay, Edith Peynaud-Debayle, Gabriel Baron, Agnès Le Querrec, Michel Dreyfus, Laurence Salomon, Vassilis Tsatsaris, Dominique de Prost, Laurent Mandelbrot, ECLAXIR study group
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/afc7d0bf34854e6daef6d618b7dd9645
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spelling	oai:doaj.org-article:afc7d0bf34854e6daef6d618b7dd96452021-11-25T06:21:42ZSearch for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.1932-620310.1371/journal.pone.0006192https://doaj.org/article/afc7d0bf34854e6daef6d618b7dd96452009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19587779/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Preeclampsia and coronary-artery disease share risk factors, suggesting common pathophysiological mechanisms. CX3CR1/CX3CL1 mediates leukocyte migration and adhesion and has been implicated in the pathophysiology of several inflammatory diseases. M280/I249 variants of CX3CR1 are associated with an atheroprotective effect and reduced endothelial dysfunction. The aim of this study was to search for an association between V249I and T280M polymorphisms of CX3CR1, preeclampsia and endothelial dysfunction.<h4>Methodology/principal findings</h4>We explored these polymorphisms with real-time polymerase chain reaction in a case-control study (184 white women with preeclampsia and 184 matched normotensive pregnant women). Endothelial dysfunction biomarkers including von Willebrand factor, VCAM-1 and thrombomodulin, as well as the soluble form of CX3CL1 were measured by enzyme-linked immunosorbent assays (ELISA). The I249 and M280 alleles were associated neither with preeclampsia, nor with its more severe form or with endothelial injury. In contrast, we found a trend toward increased CX3CL1 levels in preeclampsia patients, especially in early-onset- preeclampsia as compared to its level in later-onset- preeclampsia.<h4>Conclusions/significance</h4>This is the first study to characterize the CX3CR1 gene polymorphisms in patients with preeclampsia. We found no differences in genotype or haplotype frequencies between patients with PE and normal pregnancies, suggesting that maternal CX3CR1 V249I and T280M polymorphisms do not increase susceptibility to preeclampsia. Further studies should be performed to directly evaluate the pathophysiological role of CX3CL1, a molecule abundantly expressed in endometrium, which has been shown to stimulate human trophoblast migration.Alain StepanianSoraya BenchenniTiphaine Beillat-LucasSophie OmnesFannie DefayEdith Peynaud-DebayleGabriel BaronAgnès Le QuerrecMichel DreyfusLaurence SalomonVassilis TsatsarisDominique de ProstLaurent MandelbrotECLAXIR study groupPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6192 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alain Stepanian Soraya Benchenni Tiphaine Beillat-Lucas Sophie Omnes Fannie Defay Edith Peynaud-Debayle Gabriel Baron Agnès Le Querrec Michel Dreyfus Laurence Salomon Vassilis Tsatsaris Dominique de Prost Laurent Mandelbrot ECLAXIR study group Search for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.
description	<h4>Background</h4>Preeclampsia and coronary-artery disease share risk factors, suggesting common pathophysiological mechanisms. CX3CR1/CX3CL1 mediates leukocyte migration and adhesion and has been implicated in the pathophysiology of several inflammatory diseases. M280/I249 variants of CX3CR1 are associated with an atheroprotective effect and reduced endothelial dysfunction. The aim of this study was to search for an association between V249I and T280M polymorphisms of CX3CR1, preeclampsia and endothelial dysfunction.<h4>Methodology/principal findings</h4>We explored these polymorphisms with real-time polymerase chain reaction in a case-control study (184 white women with preeclampsia and 184 matched normotensive pregnant women). Endothelial dysfunction biomarkers including von Willebrand factor, VCAM-1 and thrombomodulin, as well as the soluble form of CX3CL1 were measured by enzyme-linked immunosorbent assays (ELISA). The I249 and M280 alleles were associated neither with preeclampsia, nor with its more severe form or with endothelial injury. In contrast, we found a trend toward increased CX3CL1 levels in preeclampsia patients, especially in early-onset- preeclampsia as compared to its level in later-onset- preeclampsia.<h4>Conclusions/significance</h4>This is the first study to characterize the CX3CR1 gene polymorphisms in patients with preeclampsia. We found no differences in genotype or haplotype frequencies between patients with PE and normal pregnancies, suggesting that maternal CX3CR1 V249I and T280M polymorphisms do not increase susceptibility to preeclampsia. Further studies should be performed to directly evaluate the pathophysiological role of CX3CL1, a molecule abundantly expressed in endometrium, which has been shown to stimulate human trophoblast migration.
format	article
author	Alain Stepanian Soraya Benchenni Tiphaine Beillat-Lucas Sophie Omnes Fannie Defay Edith Peynaud-Debayle Gabriel Baron Agnès Le Querrec Michel Dreyfus Laurence Salomon Vassilis Tsatsaris Dominique de Prost Laurent Mandelbrot ECLAXIR study group
author_facet	Alain Stepanian Soraya Benchenni Tiphaine Beillat-Lucas Sophie Omnes Fannie Defay Edith Peynaud-Debayle Gabriel Baron Agnès Le Querrec Michel Dreyfus Laurence Salomon Vassilis Tsatsaris Dominique de Prost Laurent Mandelbrot ECLAXIR study group
author_sort	Alain Stepanian
title	Search for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.
title_short	Search for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.
title_full	Search for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.
title_fullStr	Search for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.
title_full_unstemmed	Search for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.
title_sort	search for an association between v249i and t280m cx3cr1 genetic polymorphisms, endothelial injury and preeclampsia: the eclaxir study.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/afc7d0bf34854e6daef6d618b7dd9645
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Search for an association between V249I and T280M CX3CR1 genetic polymorphisms, endothelial injury and preeclampsia: the ECLAXIR study.

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