Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress
Jiang Zhu,1,* Xia Zhang,2,* Hong Xie,1 Yuye Wang,1 Xiaoxiao Zhang,1 Zhaoheng Lin3 1Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, Suzhou, 215008, Jiangsu, People’s Republic of China; 2Department of Anesthesiology, Wuzhong People’s Hospital, Suzhou...
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oai:doaj.org-article:afcb49be66584d79943dc9e47a71fa092021-12-02T16:28:08ZCardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress1178-7031https://doaj.org/article/afcb49be66584d79943dc9e47a71fa092021-08-01T00:00:00Zhttps://www.dovepress.com/cardiomyocyte-stim1-deficiency-exacerbates-doxorubicin-cardiotoxicity--peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Jiang Zhu,1,* Xia Zhang,2,* Hong Xie,1 Yuye Wang,1 Xiaoxiao Zhang,1 Zhaoheng Lin3 1Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, Suzhou, 215008, Jiangsu, People’s Republic of China; 2Department of Anesthesiology, Wuzhong People’s Hospital, Suzhou, Jiangsu, 215128, People’s Republic of China; 3Intensive Care Unit, People’s Hospital of Xishuangbanna Dai Nationality Autonomous Prefecture, Jinghong City, 666100, Yunnan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhaoheng LinIntensive Care Unit, People’s Hospital of Xishuangbanna Dai Nationality Autonomous Prefecture, Galan South Road, Jinghong City, 666100, Yunnan, People’s Republic of ChinaTel +86691-2123836Fax +8615198406999Email linzh_med@163.comIntroduction: Doxorubicin (Dox) is an effective anticancer agent; however, its cardiotoxicity remains a challenge. Dysfunction of intracellular calcium ion (Ca2+) is implicated in the process of Dox-induced cardiomyocyte apoptosis. Although store-operated Ca2+ entry (SOCE) is suggested to be responsible for Ca2+ entry in cardiomyocytes, the direct role of store-operated Ca2+ channels in Dox-related cardiomyocyte apoptosis is unknown.Materials and Methods: Cardiomyocyte Stim1-specific knockout or overexpression mice were treated with Dox. Cardiomyocytes were pretreated with Stim1 adenovirus or siRNA followed by Dox incubation in vitro. Cardiac function and underlying mechanisms echocardiography were assessed via immunofluorescence, flow cytometry, real-time PCR, Western blotting and immunoprecipitation.Results: We observed the inhibition of Stim1 expression, association of Stim1 to Orai1 or Trpc1, and SOCE in Dox-treated mouse myocardium and cardiomyocytes. Orai1 and Trpc1 expression remained unchanged. Cardiomyocyte-specific deficiency of Stim1 exacerbated Dox-induced cardiac dysfunction and myocardial apoptosis. However, specific overexpression of Stim1 in the myocardium was associated with amelioration of cardiac dysfunction and myocardial apoptosis. In vitro, STIM1 knockdown potentiated Dox-induced AC16 human cardiomyocyte apoptosis. This apoptosis was attenuated by STIM1 upregulation. Moreover, STIM1 downregulation enhanced Dox-induced endoplasmic reticulum (ER) stress in cardiomyocytes. In contrast, STIM1 overexpression inhibited the activation of the above molecular markers of ER stress. Immunoprecipitation assay showed that STIM1 interacted with GRP78 in cardiomyocytes. This interaction was attenuated in response to Dox treatment.Conclusion: Our data demonstrate that cardiomyocyte STIM1 binding to GRP78 ameliorates Dox cardiotoxicity by inhibiting pro-apoptotic ER stress.Keywords: doxorubicin, cardiotoxicity, cardiomyocyte apoptosis, STIM1, endoplasmic reticulum stressZhu JZhang XXie HWang YZhang XLin ZDove Medical Pressarticledoxorubicincardiotoxicitycardiomyocyte apoptosisstim1endoplasmic reticulum stressPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 3945-3958 (2021) |
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doxorubicin cardiotoxicity cardiomyocyte apoptosis stim1 endoplasmic reticulum stress Pathology RB1-214 Therapeutics. Pharmacology RM1-950 |
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doxorubicin cardiotoxicity cardiomyocyte apoptosis stim1 endoplasmic reticulum stress Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Zhu J Zhang X Xie H Wang Y Zhang X Lin Z Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress |
| description |
Jiang Zhu,1,* Xia Zhang,2,* Hong Xie,1 Yuye Wang,1 Xiaoxiao Zhang,1 Zhaoheng Lin3 1Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, Suzhou, 215008, Jiangsu, People’s Republic of China; 2Department of Anesthesiology, Wuzhong People’s Hospital, Suzhou, Jiangsu, 215128, People’s Republic of China; 3Intensive Care Unit, People’s Hospital of Xishuangbanna Dai Nationality Autonomous Prefecture, Jinghong City, 666100, Yunnan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhaoheng LinIntensive Care Unit, People’s Hospital of Xishuangbanna Dai Nationality Autonomous Prefecture, Galan South Road, Jinghong City, 666100, Yunnan, People’s Republic of ChinaTel +86691-2123836Fax +8615198406999Email linzh_med@163.comIntroduction: Doxorubicin (Dox) is an effective anticancer agent; however, its cardiotoxicity remains a challenge. Dysfunction of intracellular calcium ion (Ca2+) is implicated in the process of Dox-induced cardiomyocyte apoptosis. Although store-operated Ca2+ entry (SOCE) is suggested to be responsible for Ca2+ entry in cardiomyocytes, the direct role of store-operated Ca2+ channels in Dox-related cardiomyocyte apoptosis is unknown.Materials and Methods: Cardiomyocyte Stim1-specific knockout or overexpression mice were treated with Dox. Cardiomyocytes were pretreated with Stim1 adenovirus or siRNA followed by Dox incubation in vitro. Cardiac function and underlying mechanisms echocardiography were assessed via immunofluorescence, flow cytometry, real-time PCR, Western blotting and immunoprecipitation.Results: We observed the inhibition of Stim1 expression, association of Stim1 to Orai1 or Trpc1, and SOCE in Dox-treated mouse myocardium and cardiomyocytes. Orai1 and Trpc1 expression remained unchanged. Cardiomyocyte-specific deficiency of Stim1 exacerbated Dox-induced cardiac dysfunction and myocardial apoptosis. However, specific overexpression of Stim1 in the myocardium was associated with amelioration of cardiac dysfunction and myocardial apoptosis. In vitro, STIM1 knockdown potentiated Dox-induced AC16 human cardiomyocyte apoptosis. This apoptosis was attenuated by STIM1 upregulation. Moreover, STIM1 downregulation enhanced Dox-induced endoplasmic reticulum (ER) stress in cardiomyocytes. In contrast, STIM1 overexpression inhibited the activation of the above molecular markers of ER stress. Immunoprecipitation assay showed that STIM1 interacted with GRP78 in cardiomyocytes. This interaction was attenuated in response to Dox treatment.Conclusion: Our data demonstrate that cardiomyocyte STIM1 binding to GRP78 ameliorates Dox cardiotoxicity by inhibiting pro-apoptotic ER stress.Keywords: doxorubicin, cardiotoxicity, cardiomyocyte apoptosis, STIM1, endoplasmic reticulum stress |
| format |
article |
| author |
Zhu J Zhang X Xie H Wang Y Zhang X Lin Z |
| author_facet |
Zhu J Zhang X Xie H Wang Y Zhang X Lin Z |
| author_sort |
Zhu J |
| title |
Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress |
| title_short |
Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress |
| title_full |
Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress |
| title_fullStr |
Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress |
| title_full_unstemmed |
Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress |
| title_sort |
cardiomyocyte stim1 deficiency exacerbates doxorubicin cardiotoxicity by magnification of endoplasmic reticulum stress |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/afcb49be66584d79943dc9e47a71fa09 |
| work_keys_str_mv |
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1718383951349809152 |