Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner

Abstract In the present study, we showed that in the retina of Drosophila, the expression of the ho gene, encoding haeme oxygenase (HO), is regulated by light but only at the beginning of the day. This timing must be set by the circadian clock as light pulses applied at other time points during the...

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Autores principales:	Milena Damulewicz, Agnieszka Loboda, Alicja Jozkowicz, Jozef Dulak, Elzbieta Pyza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/afccc3a3edfa4d41abba522dc59cb69d
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spelling	oai:doaj.org-article:afccc3a3edfa4d41abba522dc59cb69d2021-12-02T12:32:00ZHaeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner10.1038/s41598-017-05418-62045-2322https://doaj.org/article/afccc3a3edfa4d41abba522dc59cb69d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05418-6https://doaj.org/toc/2045-2322Abstract In the present study, we showed that in the retina of Drosophila, the expression of the ho gene, encoding haeme oxygenase (HO), is regulated by light but only at the beginning of the day. This timing must be set by the circadian clock as light pulses applied at other time points during the day do not increase the ho mRNA level. Moreover, light-induced activation of HO does not depend on the canonical phototransduction pathway but instead involves cryptochrome and is enhanced by ultraviolet (UV) light. Interestingly, the level of DNA damage in the retina after UV exposure was inversely related to the circadian oscillation of the ho mRNA level during the night, being the highest when the HO level was low and reversed during the day. Accordingly, induction of HO by hemin was associated with low DNA damage, while inhibition of HO activity by SnPPIX aggravated the damage. Our data suggest that HO acts in the retina to decrease oxidative DNA damage in photoreceptors caused by UV-rich light in the morning.Milena DamulewiczAgnieszka LobodaAlicja JozkowiczJozef DulakElzbieta PyzaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Milena Damulewicz Agnieszka Loboda Alicja Jozkowicz Jozef Dulak Elzbieta Pyza Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner
description	Abstract In the present study, we showed that in the retina of Drosophila, the expression of the ho gene, encoding haeme oxygenase (HO), is regulated by light but only at the beginning of the day. This timing must be set by the circadian clock as light pulses applied at other time points during the day do not increase the ho mRNA level. Moreover, light-induced activation of HO does not depend on the canonical phototransduction pathway but instead involves cryptochrome and is enhanced by ultraviolet (UV) light. Interestingly, the level of DNA damage in the retina after UV exposure was inversely related to the circadian oscillation of the ho mRNA level during the night, being the highest when the HO level was low and reversed during the day. Accordingly, induction of HO by hemin was associated with low DNA damage, while inhibition of HO activity by SnPPIX aggravated the damage. Our data suggest that HO acts in the retina to decrease oxidative DNA damage in photoreceptors caused by UV-rich light in the morning.
format	article
author	Milena Damulewicz Agnieszka Loboda Alicja Jozkowicz Jozef Dulak Elzbieta Pyza
author_facet	Milena Damulewicz Agnieszka Loboda Alicja Jozkowicz Jozef Dulak Elzbieta Pyza
author_sort	Milena Damulewicz
title	Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner
title_short	Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner
title_full	Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner
title_fullStr	Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner
title_full_unstemmed	Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner
title_sort	haeme oxygenase protects against uv light dna damages in the retina in clock-dependent manner
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/afccc3a3edfa4d41abba522dc59cb69d
work_keys_str_mv	AT milenadamulewicz haemeoxygenaseprotectsagainstuvlightdnadamagesintheretinainclockdependentmanner AT agnieszkaloboda haemeoxygenaseprotectsagainstuvlightdnadamagesintheretinainclockdependentmanner AT alicjajozkowicz haemeoxygenaseprotectsagainstuvlightdnadamagesintheretinainclockdependentmanner AT jozefdulak haemeoxygenaseprotectsagainstuvlightdnadamagesintheretinainclockdependentmanner AT elzbietapyza haemeoxygenaseprotectsagainstuvlightdnadamagesintheretinainclockdependentmanner
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Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner

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