The cyst-dividing bacterium Ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment.
Ramlibacter tataouinensis TTB310(T) (strain TTB310), a betaproteobacterium isolated from a semi-arid region of South Tunisia (Tataouine), is characterized by the presence of both spherical and rod-shaped cells in pure culture. Cell division of strain TTB310 occurs by the binary fission of spherical...
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oai:doaj.org-article:afd0448274e24149bb9d29b455e3013f2021-11-18T06:46:48ZThe cyst-dividing bacterium Ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment.1932-620310.1371/journal.pone.0023784https://doaj.org/article/afd0448274e24149bb9d29b455e3013f2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21912644/?tool=EBIhttps://doaj.org/toc/1932-6203Ramlibacter tataouinensis TTB310(T) (strain TTB310), a betaproteobacterium isolated from a semi-arid region of South Tunisia (Tataouine), is characterized by the presence of both spherical and rod-shaped cells in pure culture. Cell division of strain TTB310 occurs by the binary fission of spherical "cyst-like" cells ("cyst-cyst" division). The rod-shaped cells formed at the periphery of a colony (consisting mainly of cysts) are highly motile and colonize a new environment, where they form a new colony by reversion to cyst-like cells. This unique cell cycle of strain TTB310, with desiccation tolerant cyst-like cells capable of division and desiccation sensitive motile rods capable of dissemination, appears to be a novel adaptation for life in a hot and dry desert environment. In order to gain insights into strain TTB310's underlying genetic repertoire and possible mechanisms responsible for its unusual lifestyle, the genome of strain TTB310 was completely sequenced and subsequently annotated. The complete genome consists of a single circular chromosome of 4,070,194 bp with an average G+C content of 70.0%, the highest among the Betaproteobacteria sequenced to date, with total of 3,899 predicted coding sequences covering 92% of the genome. We found that strain TTB310 has developed a highly complex network of two-component systems, which may utilize responses to light and perhaps a rudimentary circadian hourglass to anticipate water availability at the dew time in the middle/end of the desert winter nights and thus direct the growth window to cyclic water availability times. Other interesting features of the strain TTB310 genome that appear to be important for desiccation tolerance, including intermediary metabolism compounds such as trehalose or polyhydroxyalkanoate, and signal transduction pathways, are presented and discussed.Gilles De LucaMohamed BarakatPhilippe OrtetSylvain FochesatoCécile Jourlin-CastelliMireille AnsaldiBéatrice PyGwennaele FichantPedro M CoutinhoRomé VoulhouxOlivier BastienEric MaréchalBernard HenrissatYves QuentinPhilippe NoirotAlain FillouxVincent MéjeanMichael S DuBowFrédéric BarrasValérie BarbeJean WeissenbachIrina MihalcescuAndré VerméglioWafa AchouakThierry HeulinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e23784 (2011) |
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Medicine R Science Q Gilles De Luca Mohamed Barakat Philippe Ortet Sylvain Fochesato Cécile Jourlin-Castelli Mireille Ansaldi Béatrice Py Gwennaele Fichant Pedro M Coutinho Romé Voulhoux Olivier Bastien Eric Maréchal Bernard Henrissat Yves Quentin Philippe Noirot Alain Filloux Vincent Méjean Michael S DuBow Frédéric Barras Valérie Barbe Jean Weissenbach Irina Mihalcescu André Verméglio Wafa Achouak Thierry Heulin The cyst-dividing bacterium Ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment. |
| description |
Ramlibacter tataouinensis TTB310(T) (strain TTB310), a betaproteobacterium isolated from a semi-arid region of South Tunisia (Tataouine), is characterized by the presence of both spherical and rod-shaped cells in pure culture. Cell division of strain TTB310 occurs by the binary fission of spherical "cyst-like" cells ("cyst-cyst" division). The rod-shaped cells formed at the periphery of a colony (consisting mainly of cysts) are highly motile and colonize a new environment, where they form a new colony by reversion to cyst-like cells. This unique cell cycle of strain TTB310, with desiccation tolerant cyst-like cells capable of division and desiccation sensitive motile rods capable of dissemination, appears to be a novel adaptation for life in a hot and dry desert environment. In order to gain insights into strain TTB310's underlying genetic repertoire and possible mechanisms responsible for its unusual lifestyle, the genome of strain TTB310 was completely sequenced and subsequently annotated. The complete genome consists of a single circular chromosome of 4,070,194 bp with an average G+C content of 70.0%, the highest among the Betaproteobacteria sequenced to date, with total of 3,899 predicted coding sequences covering 92% of the genome. We found that strain TTB310 has developed a highly complex network of two-component systems, which may utilize responses to light and perhaps a rudimentary circadian hourglass to anticipate water availability at the dew time in the middle/end of the desert winter nights and thus direct the growth window to cyclic water availability times. Other interesting features of the strain TTB310 genome that appear to be important for desiccation tolerance, including intermediary metabolism compounds such as trehalose or polyhydroxyalkanoate, and signal transduction pathways, are presented and discussed. |
| format |
article |
| author |
Gilles De Luca Mohamed Barakat Philippe Ortet Sylvain Fochesato Cécile Jourlin-Castelli Mireille Ansaldi Béatrice Py Gwennaele Fichant Pedro M Coutinho Romé Voulhoux Olivier Bastien Eric Maréchal Bernard Henrissat Yves Quentin Philippe Noirot Alain Filloux Vincent Méjean Michael S DuBow Frédéric Barras Valérie Barbe Jean Weissenbach Irina Mihalcescu André Verméglio Wafa Achouak Thierry Heulin |
| author_facet |
Gilles De Luca Mohamed Barakat Philippe Ortet Sylvain Fochesato Cécile Jourlin-Castelli Mireille Ansaldi Béatrice Py Gwennaele Fichant Pedro M Coutinho Romé Voulhoux Olivier Bastien Eric Maréchal Bernard Henrissat Yves Quentin Philippe Noirot Alain Filloux Vincent Méjean Michael S DuBow Frédéric Barras Valérie Barbe Jean Weissenbach Irina Mihalcescu André Verméglio Wafa Achouak Thierry Heulin |
| author_sort |
Gilles De Luca |
| title |
The cyst-dividing bacterium Ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment. |
| title_short |
The cyst-dividing bacterium Ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment. |
| title_full |
The cyst-dividing bacterium Ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment. |
| title_fullStr |
The cyst-dividing bacterium Ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment. |
| title_full_unstemmed |
The cyst-dividing bacterium Ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment. |
| title_sort |
cyst-dividing bacterium ramlibacter tataouinensis ttb310 genome reveals a well-stocked toolbox for adaptation to a desert environment. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/afd0448274e24149bb9d29b455e3013f |
| work_keys_str_mv |
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