Polydopamine Coating-Mediated Immobilization of BMP-2 on Polyethylene Terephthalate-Based Artificial Ligaments for Enhanced Bioactivity
Background/objectives: Polyethylene terephthalate (PET)-based artificial ligaments are one of the most commonly used grafts in anterior cruciate ligament (ACL) reconstruction surgery. However, the lack of favorable hydrophilicity and cell attachment for PET highly impeded its widespread application...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/afd60326aab24cc2a0dee41a02cf3512 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:afd60326aab24cc2a0dee41a02cf3512 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:afd60326aab24cc2a0dee41a02cf35122021-11-16T07:46:25ZPolydopamine Coating-Mediated Immobilization of BMP-2 on Polyethylene Terephthalate-Based Artificial Ligaments for Enhanced Bioactivity2296-418510.3389/fbioe.2021.749221https://doaj.org/article/afd60326aab24cc2a0dee41a02cf35122021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.749221/fullhttps://doaj.org/toc/2296-4185Background/objectives: Polyethylene terephthalate (PET)-based artificial ligaments are one of the most commonly used grafts in anterior cruciate ligament (ACL) reconstruction surgery. However, the lack of favorable hydrophilicity and cell attachment for PET highly impeded its widespread application in clinical practice. Studies found that surface modification on PET materials could enhance the biocompatibility and bioactivity of PET ligaments. In this study, we immobilized bone morphogenetic protein-2 (BMP-2) on the surface of PET ligaments mediated by polydopamine (PDA) coating and investigated the bioactivation and graft-to-bone healing effect of the modified grafts in vivo and in vitro.Methods: In this study, we prepared the PDA coating and subsequent BMP-2-immobilized PET artificial ligaments. Scanning electron microscopy (SEM) was used to analyze the morphological changes of the modified grafts. In addition, the surface wettability properties of the modified ligaments, amount of immobilized BMP 2, and the release of BMP-2 during a dynamic period up to 28 days were tested. Then, the attachment and proliferation of rat bone mesenchymal stem cells (rBMSCs) on grafts were examined by SEM and Cell Counting Kit-8 (CCK-8) assay, respectively. Alkaline phosphatase (ALP) assay, RT-PCR, and Alizarin Red S staining were performed to test the osteoinduction property. For in vivo experiments, an extra-articular graft-to-bone healing model in rabbits was established. At 8 weeks after surgery, biomechanical tests, micro-CT, and histological staining were performed on harvested samples.Results: A surface morphological analysis verified the success of the PDA coating. The wettability of the PET artificial ligaments was improved, and more than 80% of BMP-2 stably remained on the graft surface for 28 days. The modified grafts could significantly enhance the proliferation, attachment, as well as expression of ALP and osteogenic-related genes, which demonstrated the favorable bioactivity of the grafts immobilized with BMP-2 in vitro. Moreover, the grafts immobilized with BMP-2 at a concentration of 138.4 ± 10.6 ng/cm2 could highly improve the biomechanical properties, bone regeneration, and healing between grafts and host bone after the implantation into the rabbits compared with the PDA-PET group or the PET group.Conclusion: The immobilization of BMP-2 mediated by polydopamine coating on PET artificial ligament surface could enhance the compatibility and bioactivity of the scaffolds and the graft-to-bone healing in vivo.Zhanrong KangDejian LiChaoqin ShuChaoqin ShuJianhang DuBin YuZhi QianZeyuan ZhongXu ZhangBaoqing YuQikai HuangJianming HuangYufang ZhuChengqing YiHuifeng DingHuifeng DingFrontiers Media S.A.articleartificial ligamentbioactivitybone morphogenic proteinsgraft-to-bone healingpolyethylene terephthalatesurface modificationBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
artificial ligament bioactivity bone morphogenic proteins graft-to-bone healing polyethylene terephthalate surface modification Biotechnology TP248.13-248.65 |
| spellingShingle |
artificial ligament bioactivity bone morphogenic proteins graft-to-bone healing polyethylene terephthalate surface modification Biotechnology TP248.13-248.65 Zhanrong Kang Dejian Li Chaoqin Shu Chaoqin Shu Jianhang Du Bin Yu Zhi Qian Zeyuan Zhong Xu Zhang Baoqing Yu Qikai Huang Jianming Huang Yufang Zhu Chengqing Yi Huifeng Ding Huifeng Ding Polydopamine Coating-Mediated Immobilization of BMP-2 on Polyethylene Terephthalate-Based Artificial Ligaments for Enhanced Bioactivity |
| description |
Background/objectives: Polyethylene terephthalate (PET)-based artificial ligaments are one of the most commonly used grafts in anterior cruciate ligament (ACL) reconstruction surgery. However, the lack of favorable hydrophilicity and cell attachment for PET highly impeded its widespread application in clinical practice. Studies found that surface modification on PET materials could enhance the biocompatibility and bioactivity of PET ligaments. In this study, we immobilized bone morphogenetic protein-2 (BMP-2) on the surface of PET ligaments mediated by polydopamine (PDA) coating and investigated the bioactivation and graft-to-bone healing effect of the modified grafts in vivo and in vitro.Methods: In this study, we prepared the PDA coating and subsequent BMP-2-immobilized PET artificial ligaments. Scanning electron microscopy (SEM) was used to analyze the morphological changes of the modified grafts. In addition, the surface wettability properties of the modified ligaments, amount of immobilized BMP 2, and the release of BMP-2 during a dynamic period up to 28 days were tested. Then, the attachment and proliferation of rat bone mesenchymal stem cells (rBMSCs) on grafts were examined by SEM and Cell Counting Kit-8 (CCK-8) assay, respectively. Alkaline phosphatase (ALP) assay, RT-PCR, and Alizarin Red S staining were performed to test the osteoinduction property. For in vivo experiments, an extra-articular graft-to-bone healing model in rabbits was established. At 8 weeks after surgery, biomechanical tests, micro-CT, and histological staining were performed on harvested samples.Results: A surface morphological analysis verified the success of the PDA coating. The wettability of the PET artificial ligaments was improved, and more than 80% of BMP-2 stably remained on the graft surface for 28 days. The modified grafts could significantly enhance the proliferation, attachment, as well as expression of ALP and osteogenic-related genes, which demonstrated the favorable bioactivity of the grafts immobilized with BMP-2 in vitro. Moreover, the grafts immobilized with BMP-2 at a concentration of 138.4 ± 10.6 ng/cm2 could highly improve the biomechanical properties, bone regeneration, and healing between grafts and host bone after the implantation into the rabbits compared with the PDA-PET group or the PET group.Conclusion: The immobilization of BMP-2 mediated by polydopamine coating on PET artificial ligament surface could enhance the compatibility and bioactivity of the scaffolds and the graft-to-bone healing in vivo. |
| format |
article |
| author |
Zhanrong Kang Dejian Li Chaoqin Shu Chaoqin Shu Jianhang Du Bin Yu Zhi Qian Zeyuan Zhong Xu Zhang Baoqing Yu Qikai Huang Jianming Huang Yufang Zhu Chengqing Yi Huifeng Ding Huifeng Ding |
| author_facet |
Zhanrong Kang Dejian Li Chaoqin Shu Chaoqin Shu Jianhang Du Bin Yu Zhi Qian Zeyuan Zhong Xu Zhang Baoqing Yu Qikai Huang Jianming Huang Yufang Zhu Chengqing Yi Huifeng Ding Huifeng Ding |
| author_sort |
Zhanrong Kang |
| title |
Polydopamine Coating-Mediated Immobilization of BMP-2 on Polyethylene Terephthalate-Based Artificial Ligaments for Enhanced Bioactivity |
| title_short |
Polydopamine Coating-Mediated Immobilization of BMP-2 on Polyethylene Terephthalate-Based Artificial Ligaments for Enhanced Bioactivity |
| title_full |
Polydopamine Coating-Mediated Immobilization of BMP-2 on Polyethylene Terephthalate-Based Artificial Ligaments for Enhanced Bioactivity |
| title_fullStr |
Polydopamine Coating-Mediated Immobilization of BMP-2 on Polyethylene Terephthalate-Based Artificial Ligaments for Enhanced Bioactivity |
| title_full_unstemmed |
Polydopamine Coating-Mediated Immobilization of BMP-2 on Polyethylene Terephthalate-Based Artificial Ligaments for Enhanced Bioactivity |
| title_sort |
polydopamine coating-mediated immobilization of bmp-2 on polyethylene terephthalate-based artificial ligaments for enhanced bioactivity |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/afd60326aab24cc2a0dee41a02cf3512 |
| work_keys_str_mv |
AT zhanrongkang polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT dejianli polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT chaoqinshu polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT chaoqinshu polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT jianhangdu polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT binyu polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT zhiqian polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT zeyuanzhong polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT xuzhang polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT baoqingyu polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT qikaihuang polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT jianminghuang polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT yufangzhu polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT chengqingyi polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT huifengding polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity AT huifengding polydopaminecoatingmediatedimmobilizationofbmp2onpolyethyleneterephthalatebasedartificialligamentsforenhancedbioactivity |
| _version_ |
1718426625383596032 |