Osteogenic activity of titanium surfaces with nanonetwork structures

Helin Xing,1,2 Satoshi Komasa,3 Yoichiro Taguchi,4 Tohru Sekino,5 Joji Okazaki3 1Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China; 2Graduate School of Dentistry (Removable Prosthodontics and Occl...

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Autores principales:	Xing H, Komasa S, Taguchi Y, Sekino T, Okazaki J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/afe30915785149c998cfe2feb89394f7
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spelling	oai:doaj.org-article:afe30915785149c998cfe2feb89394f72021-12-02T07:13:44ZOsteogenic activity of titanium surfaces with nanonetwork structures1178-2013https://doaj.org/article/afe30915785149c998cfe2feb89394f72014-04-01T00:00:00Zhttp://www.dovepress.com/osteogenic-activity-of-titanium-surfaces-with-nanonetwork-structures-a16366https://doaj.org/toc/1178-2013 Helin Xing,1,2 Satoshi Komasa,3 Yoichiro Taguchi,4 Tohru Sekino,5 Joji Okazaki3 1Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China; 2Graduate School of Dentistry (Removable Prosthodontics and Occlusion), 3Department of Removable Prosthodontics and Occlusion, 4Department of Periodontology, Osaka Dental University, Hirakata, Osaka, Japan; 5Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba-ku, Sendai, Japan Background: Titanium surfaces play an important role in affecting osseointegration of dental implants. Previous studies have shown that the titania nanotube promotes osseointegration by enhancing osteogenic differentiation. Only relatively recently have the effects of titanium surfaces with other nanostructures on osteogenic differentiation been investigated. Methods: In this study, we used NaOH solutions with concentrations of 2.5, 5.0, 7.5, 10.0, and 12.5 M to develop a simple and useful titanium surface modification that introduces the nanonetwork structures with titania nanosheet (TNS) nanofeatures to the surface of titanium disks. The effects of such a modified nanonetwork structure, with different alkaline concentrations on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMMSCs), were evaluated. Results: The nanonetwork structures with TNS nanofeatures induced by alkali etching markedly enhanced BMMSC functions of cell adhesion and osteogenesis-related gene expression, and other cell behaviors such as proliferation, alkaline phosphatase activity, extracellular matrix deposition, and mineralization were also significantly increased. These effects were most pronounced when the concentration of NaOH was 10.0 M. Conclusion: The results suggest that nanonetwork structures with TNS nanofeatures improved BMMSC proliferation and induced BMMSC osteogenic differentiation. In addition, the surfaces formed with 10.0 M NaOH suggest the potential to improve the clinical performance of dental implants. Keywords: nanotopography, osseointegration, surface modification, bone marrow mesenchymal stem cellsXing HKomasa STaguchi YSekino TOkazaki JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1741-1755 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Xing H Komasa S Taguchi Y Sekino T Okazaki J Osteogenic activity of titanium surfaces with nanonetwork structures
description	Helin Xing,1,2 Satoshi Komasa,3 Yoichiro Taguchi,4 Tohru Sekino,5 Joji Okazaki3 1Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China; 2Graduate School of Dentistry (Removable Prosthodontics and Occlusion), 3Department of Removable Prosthodontics and Occlusion, 4Department of Periodontology, Osaka Dental University, Hirakata, Osaka, Japan; 5Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba-ku, Sendai, Japan Background: Titanium surfaces play an important role in affecting osseointegration of dental implants. Previous studies have shown that the titania nanotube promotes osseointegration by enhancing osteogenic differentiation. Only relatively recently have the effects of titanium surfaces with other nanostructures on osteogenic differentiation been investigated. Methods: In this study, we used NaOH solutions with concentrations of 2.5, 5.0, 7.5, 10.0, and 12.5 M to develop a simple and useful titanium surface modification that introduces the nanonetwork structures with titania nanosheet (TNS) nanofeatures to the surface of titanium disks. The effects of such a modified nanonetwork structure, with different alkaline concentrations on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMMSCs), were evaluated. Results: The nanonetwork structures with TNS nanofeatures induced by alkali etching markedly enhanced BMMSC functions of cell adhesion and osteogenesis-related gene expression, and other cell behaviors such as proliferation, alkaline phosphatase activity, extracellular matrix deposition, and mineralization were also significantly increased. These effects were most pronounced when the concentration of NaOH was 10.0 M. Conclusion: The results suggest that nanonetwork structures with TNS nanofeatures improved BMMSC proliferation and induced BMMSC osteogenic differentiation. In addition, the surfaces formed with 10.0 M NaOH suggest the potential to improve the clinical performance of dental implants. Keywords: nanotopography, osseointegration, surface modification, bone marrow mesenchymal stem cells
format	article
author	Xing H Komasa S Taguchi Y Sekino T Okazaki J
author_facet	Xing H Komasa S Taguchi Y Sekino T Okazaki J
author_sort	Xing H
title	Osteogenic activity of titanium surfaces with nanonetwork structures
title_short	Osteogenic activity of titanium surfaces with nanonetwork structures
title_full	Osteogenic activity of titanium surfaces with nanonetwork structures
title_fullStr	Osteogenic activity of titanium surfaces with nanonetwork structures
title_full_unstemmed	Osteogenic activity of titanium surfaces with nanonetwork structures
title_sort	osteogenic activity of titanium surfaces with nanonetwork structures
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/afe30915785149c998cfe2feb89394f7
work_keys_str_mv	AT xingh osteogenicactivityoftitaniumsurfaceswithnanonetworkstructures AT komasas osteogenicactivityoftitaniumsurfaceswithnanonetworkstructures AT taguchiy osteogenicactivityoftitaniumsurfaceswithnanonetworkstructures AT sekinot osteogenicactivityoftitaniumsurfaceswithnanonetworkstructures AT okazakij osteogenicactivityoftitaniumsurfaceswithnanonetworkstructures
_version_	1718399533815169024

Osteogenic activity of titanium surfaces with nanonetwork structures

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