Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4

Abstract Background and objectives MicroRNA (miRNA) that translocate from the nucleus to mitochondria are referred to as mitochondrial microRNA (mitomiR). Albeit mitomiRs have been shown to modulate gene expression, their functional impact within mitochondria is unknown. The main objective of this s...

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Autores principales:	Praveen Sharma, Vibhuti Sharma, Tarunveer Singh Ahluwalia, Nilambra Dogra, Santosh Kumar, Sandeep Singh
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Mitochondria Metabolic reprogramming Mito-miRs Glycolysis Cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/afea08b00de54bb385b01b9322bff6f4
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spelling	oai:doaj.org-article:afea08b00de54bb385b01b9322bff6f42021-11-28T12:36:50ZLet-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND410.1186/s12935-021-02339-31475-2867https://doaj.org/article/afea08b00de54bb385b01b9322bff6f42021-11-01T00:00:00Zhttps://doi.org/10.1186/s12935-021-02339-3https://doaj.org/toc/1475-2867Abstract Background and objectives MicroRNA (miRNA) that translocate from the nucleus to mitochondria are referred to as mitochondrial microRNA (mitomiR). Albeit mitomiRs have been shown to modulate gene expression, their functional impact within mitochondria is unknown. The main objective of this study is to investigate whether the mitochondrial genome is regulated by miR present inside the mitochondria. Methods and results Here, we report mitomiR let-7a regulates mitochondrial transcription in breast cancer cells and reprogram the metabolism accordingly. These effects were mediated through the interaction of let-7a with mtDNA, as studied by RNA pull-down assays, altering the activity of Complex I in a cell line-specific manner. Our study, for the first time, identifies the role of mitomiR (let-7a) in regulating the mitochondrial genome by transcriptional repression and its contribution to regulating mitochondrial metabolism of breast cancer cells. Conclusion These findings uncover a novel mechanism by which mitomiR regulates mitochondrial transcription.Praveen SharmaVibhuti SharmaTarunveer Singh AhluwaliaNilambra DograSantosh KumarSandeep SinghBMCarticleMitochondriaMetabolic reprogrammingMito-miRsGlycolysisCancerNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCancer Cell International, Vol 21, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mitochondria Metabolic reprogramming Mito-miRs Glycolysis Cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	Mitochondria Metabolic reprogramming Mito-miRs Glycolysis Cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Praveen Sharma Vibhuti Sharma Tarunveer Singh Ahluwalia Nilambra Dogra Santosh Kumar Sandeep Singh Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4
description	Abstract Background and objectives MicroRNA (miRNA) that translocate from the nucleus to mitochondria are referred to as mitochondrial microRNA (mitomiR). Albeit mitomiRs have been shown to modulate gene expression, their functional impact within mitochondria is unknown. The main objective of this study is to investigate whether the mitochondrial genome is regulated by miR present inside the mitochondria. Methods and results Here, we report mitomiR let-7a regulates mitochondrial transcription in breast cancer cells and reprogram the metabolism accordingly. These effects were mediated through the interaction of let-7a with mtDNA, as studied by RNA pull-down assays, altering the activity of Complex I in a cell line-specific manner. Our study, for the first time, identifies the role of mitomiR (let-7a) in regulating the mitochondrial genome by transcriptional repression and its contribution to regulating mitochondrial metabolism of breast cancer cells. Conclusion These findings uncover a novel mechanism by which mitomiR regulates mitochondrial transcription.
format	article
author	Praveen Sharma Vibhuti Sharma Tarunveer Singh Ahluwalia Nilambra Dogra Santosh Kumar Sandeep Singh
author_facet	Praveen Sharma Vibhuti Sharma Tarunveer Singh Ahluwalia Nilambra Dogra Santosh Kumar Sandeep Singh
author_sort	Praveen Sharma
title	Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4
title_short	Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4
title_full	Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4
title_fullStr	Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4
title_full_unstemmed	Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4
title_sort	let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded nd4
publisher	BMC
publishDate	2021
url	https://doaj.org/article/afea08b00de54bb385b01b9322bff6f4
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Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4

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