Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients

Abstract In tumor cells, higher expression of glucose transporter proteins (GLUT) and carbonic anhydrases (CAIX) genes is influenced by hypoxia-induced factors (HIF).Thus, we aimed to study the expression profile of these markers in sequential peripheral blood collections performed in breast cancer...

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Autores principales:	Carlos Henrique F. Peiró, Matheus M. Perez, Glauco S. A. de Aquino, Jéssica F. A. Encinas, Luiz Vinícius de A. Sousa, Glaucia Luciano da Veiga, Auro del Giglio, Fernando L. A. Fonseca, Beatriz da Costa Aguiar Alves
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b000ba4ab4ba4db3ba72a2c6eedff2e4
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spelling	oai:doaj.org-article:b000ba4ab4ba4db3ba72a2c6eedff2e42021-12-02T17:32:59ZDiagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients10.1038/s41598-021-87897-22045-2322https://doaj.org/article/b000ba4ab4ba4db3ba72a2c6eedff2e42021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87897-2https://doaj.org/toc/2045-2322Abstract In tumor cells, higher expression of glucose transporter proteins (GLUT) and carbonic anhydrases (CAIX) genes is influenced by hypoxia-induced factors (HIF).Thus, we aimed to study the expression profile of these markers in sequential peripheral blood collections performed in breast cancer patients in order to verify their predictive potential in liquid biopsies. Gene expressions were analyzed by qPCR in tumor and blood samples from 125 patients and 25 healthy women. Differential expression was determined by the 2(−ΔCq) method. Expression of HIF-1α and GLUT1 in the blood of breast cancer patients is significantly higher (90–91 and 160–161 fold increased expression, respectively; p < 0.0001) than that found in healthy women. Their diagnostic power was confirmed by ROC curve. CAIX is also more expressed in breast cancer women blood, but its expression was detected only in a few samples. But none of these genes could be considered predictive markers. Therefore, evaluation of the expression of HIF-1α and GLUT1 in blood may be a useful laboratory tool to complement the diagnosis of breast cancer, in addition to being useful for follow-up of patients and of women with a family history of breast cancer.Carlos Henrique F. PeiróMatheus M. PerezGlauco S. A. de AquinoJéssica F. A. EncinasLuiz Vinícius de A. SousaGlaucia Luciano da VeigaAuro del GiglioFernando L. A. FonsecaBeatriz da Costa Aguiar AlvesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carlos Henrique F. Peiró Matheus M. Perez Glauco S. A. de Aquino Jéssica F. A. Encinas Luiz Vinícius de A. Sousa Glaucia Luciano da Veiga Auro del Giglio Fernando L. A. Fonseca Beatriz da Costa Aguiar Alves Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients
description	Abstract In tumor cells, higher expression of glucose transporter proteins (GLUT) and carbonic anhydrases (CAIX) genes is influenced by hypoxia-induced factors (HIF).Thus, we aimed to study the expression profile of these markers in sequential peripheral blood collections performed in breast cancer patients in order to verify their predictive potential in liquid biopsies. Gene expressions were analyzed by qPCR in tumor and blood samples from 125 patients and 25 healthy women. Differential expression was determined by the 2(−ΔCq) method. Expression of HIF-1α and GLUT1 in the blood of breast cancer patients is significantly higher (90–91 and 160–161 fold increased expression, respectively; p < 0.0001) than that found in healthy women. Their diagnostic power was confirmed by ROC curve. CAIX is also more expressed in breast cancer women blood, but its expression was detected only in a few samples. But none of these genes could be considered predictive markers. Therefore, evaluation of the expression of HIF-1α and GLUT1 in blood may be a useful laboratory tool to complement the diagnosis of breast cancer, in addition to being useful for follow-up of patients and of women with a family history of breast cancer.
format	article
author	Carlos Henrique F. Peiró Matheus M. Perez Glauco S. A. de Aquino Jéssica F. A. Encinas Luiz Vinícius de A. Sousa Glaucia Luciano da Veiga Auro del Giglio Fernando L. A. Fonseca Beatriz da Costa Aguiar Alves
author_facet	Carlos Henrique F. Peiró Matheus M. Perez Glauco S. A. de Aquino Jéssica F. A. Encinas Luiz Vinícius de A. Sousa Glaucia Luciano da Veiga Auro del Giglio Fernando L. A. Fonseca Beatriz da Costa Aguiar Alves
author_sort	Carlos Henrique F. Peiró
title	Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients
title_short	Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients
title_full	Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients
title_fullStr	Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients
title_full_unstemmed	Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients
title_sort	diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b000ba4ab4ba4db3ba72a2c6eedff2e4
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Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients

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