First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)
Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase (<i>pol</i>) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses (<i>Urva auropunctata</i>) on the Caribbean island of St. Kitts. All 17 PCR amplicons...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/b00279a9d4ea42ebbb8e1178c7993516 |
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| Sumario: | Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase (<i>pol</i>) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses (<i>Urva auropunctata</i>) on the Caribbean island of St. Kitts. All 17 PCR amplicons were sequenced for the partial <i>pol</i> gene (~300 bp, hereafter referred to as Mon sequences). Fourteen of the 17 Mon sequences shared maximum homology (98.3–99.6% and 97–98.9% nucleotide (nt) and deduced amino acid (aa) sequence identities, respectively) with that of bovine adenovirus-6 (species <i>Bovine atadenovirus E</i>). Mongoose-associated adenovirus Mon-39 was most closely related (absolute nt and deduced aa identities) to an atadenovirus from a tropical screech owl. Mon-66 shared maximum nt and deduced aa identities of 69% and 71.4% with those of atadenoviruses from a spur-thighed tortoise and a brown anole lizard, respectively. Phylogenetically, Mon-39 and Mon-66 clustered within clades that were predominated by atadenoviruses from reptiles, indicating a reptilian origin of these viruses. Only a single mongoose-associated adenovirus, Mon-34, was related to the genus <i>Mastadenovirus</i>. However, phylogenetically, Mon-34 formed an isolated branch, distinct from other mastadenoviruses. Since the fecal samples were collected from apparently healthy mongooses, we could not determine whether the mongoose-associated adenoviruses infected the host. On the other hand, the phylogenetic clustering patterns of the mongoose-associated atadenoviruses pointed more towards a dietary origin of these viruses. Although the present study was based on partial pol sequences (~90 aa), sequence identities and phylogenetic analysis suggested that Mon-34, Mon-39, and Mon-66 might represent novel adenoviruses. To our knowledge, this is the first report on the detection and molecular characterization of adenoviruses from the mongoose. |
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