First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (Urva auropunctata)

Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase (pol) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses (Urva auropunctata) on the Caribbean island of St. Kitts. All 17 PCR amplicons...

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Autores principales:	Kerry Gainor, Anne A. M. J. Becker, Yashpal S. Malik, Souvik Ghosh
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adenovirus small Indian mongoose atadenovirus mastadenovirus DNA-dependent DNA polymerase (pol) Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b00279a9d4ea42ebbb8e1178c7993516
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spelling	oai:doaj.org-article:b00279a9d4ea42ebbb8e1178c79935162021-11-25T19:13:14ZFirst Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)10.3390/v131121941999-4915https://doaj.org/article/b00279a9d4ea42ebbb8e1178c79935162021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2194https://doaj.org/toc/1999-4915Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase (<i>pol</i>) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses (<i>Urva auropunctata</i>) on the Caribbean island of St. Kitts. All 17 PCR amplicons were sequenced for the partial <i>pol</i> gene (~300 bp, hereafter referred to as Mon sequences). Fourteen of the 17 Mon sequences shared maximum homology (98.3–99.6% and 97–98.9% nucleotide (nt) and deduced amino acid (aa) sequence identities, respectively) with that of bovine adenovirus-6 (species <i>Bovine atadenovirus E</i>). Mongoose-associated adenovirus Mon-39 was most closely related (absolute nt and deduced aa identities) to an atadenovirus from a tropical screech owl. Mon-66 shared maximum nt and deduced aa identities of 69% and 71.4% with those of atadenoviruses from a spur-thighed tortoise and a brown anole lizard, respectively. Phylogenetically, Mon-39 and Mon-66 clustered within clades that were predominated by atadenoviruses from reptiles, indicating a reptilian origin of these viruses. Only a single mongoose-associated adenovirus, Mon-34, was related to the genus <i>Mastadenovirus</i>. However, phylogenetically, Mon-34 formed an isolated branch, distinct from other mastadenoviruses. Since the fecal samples were collected from apparently healthy mongooses, we could not determine whether the mongoose-associated adenoviruses infected the host. On the other hand, the phylogenetic clustering patterns of the mongoose-associated atadenoviruses pointed more towards a dietary origin of these viruses. Although the present study was based on partial pol sequences (~90 aa), sequence identities and phylogenetic analysis suggested that Mon-34, Mon-39, and Mon-66 might represent novel adenoviruses. To our knowledge, this is the first report on the detection and molecular characterization of adenoviruses from the mongoose.Kerry GainorAnne A. M. J. BeckerYashpal S. MalikSouvik GhoshMDPI AGarticleadenovirussmall Indian mongooseatadenovirusmastadenovirusDNA-dependent DNA polymerase (pol)MicrobiologyQR1-502ENViruses, Vol 13, Iss 2194, p 2194 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adenovirus small Indian mongoose atadenovirus mastadenovirus DNA-dependent DNA polymerase (pol) Microbiology QR1-502
spellingShingle	adenovirus small Indian mongoose atadenovirus mastadenovirus DNA-dependent DNA polymerase (pol) Microbiology QR1-502 Kerry Gainor Anne A. M. J. Becker Yashpal S. Malik Souvik Ghosh First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)
description	Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase (<i>pol</i>) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses (<i>Urva auropunctata</i>) on the Caribbean island of St. Kitts. All 17 PCR amplicons were sequenced for the partial <i>pol</i> gene (~300 bp, hereafter referred to as Mon sequences). Fourteen of the 17 Mon sequences shared maximum homology (98.3–99.6% and 97–98.9% nucleotide (nt) and deduced amino acid (aa) sequence identities, respectively) with that of bovine adenovirus-6 (species <i>Bovine atadenovirus E</i>). Mongoose-associated adenovirus Mon-39 was most closely related (absolute nt and deduced aa identities) to an atadenovirus from a tropical screech owl. Mon-66 shared maximum nt and deduced aa identities of 69% and 71.4% with those of atadenoviruses from a spur-thighed tortoise and a brown anole lizard, respectively. Phylogenetically, Mon-39 and Mon-66 clustered within clades that were predominated by atadenoviruses from reptiles, indicating a reptilian origin of these viruses. Only a single mongoose-associated adenovirus, Mon-34, was related to the genus <i>Mastadenovirus</i>. However, phylogenetically, Mon-34 formed an isolated branch, distinct from other mastadenoviruses. Since the fecal samples were collected from apparently healthy mongooses, we could not determine whether the mongoose-associated adenoviruses infected the host. On the other hand, the phylogenetic clustering patterns of the mongoose-associated atadenoviruses pointed more towards a dietary origin of these viruses. Although the present study was based on partial pol sequences (~90 aa), sequence identities and phylogenetic analysis suggested that Mon-34, Mon-39, and Mon-66 might represent novel adenoviruses. To our knowledge, this is the first report on the detection and molecular characterization of adenoviruses from the mongoose.
format	article
author	Kerry Gainor Anne A. M. J. Becker Yashpal S. Malik Souvik Ghosh
author_facet	Kerry Gainor Anne A. M. J. Becker Yashpal S. Malik Souvik Ghosh
author_sort	Kerry Gainor
title	First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)
title_short	First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)
title_full	First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)
title_fullStr	First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)
title_full_unstemmed	First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)
title_sort	first report on detection and molecular characterization of adenoviruses in the small indian mongoose (<i>urva auropunctata</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b00279a9d4ea42ebbb8e1178c7993516
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First Report on Detection and Molecular Characterization of Adenoviruses in the Small Indian Mongoose (<i>Urva auropunctata</i>)

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