Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease

Abstract Three-dimensional tissue-structural relationships are not well captured by typical thin-section histology, posing challenges for the study of tissue physiology and pathology. Moreover, while recent progress has been made with intact methods for clearing, labeling, and imaging whole organs s...

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Autores principales:	Brian Hsueh, Vanessa M. Burns, Philip Pauerstein, Katherine Holzem, Li Ye, Kristin Engberg, Ai-Chi Wang, Xueying Gu, Harini Chakravarthy, H. Efsun Arda, Gregory Charville, Hannes Vogel, Igor R. Efimov, Seung Kim, Karl Deisseroth
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b005b26d2af84e9196f1657c71463209
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spelling	oai:doaj.org-article:b005b26d2af84e9196f1657c714632092021-12-02T12:30:51ZPathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease10.1038/s41598-017-05614-42045-2322https://doaj.org/article/b005b26d2af84e9196f1657c714632092017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05614-4https://doaj.org/toc/2045-2322Abstract Three-dimensional tissue-structural relationships are not well captured by typical thin-section histology, posing challenges for the study of tissue physiology and pathology. Moreover, while recent progress has been made with intact methods for clearing, labeling, and imaging whole organs such as the mature brain, these approaches are generally unsuitable for soft, irregular, and heterogeneous tissues that account for the vast majority of clinical samples and biopsies. Here we develop a biphasic hydrogel methodology, which along with automated analysis, provides for high-throughput quantitative volumetric interrogation of spatially-irregular and friable tissue structures. We validate and apply this approach in the examination of a variety of developing and diseased tissues, with specific focus on the dynamics of normal and pathological pancreatic innervation and development, including in clinical samples. Quantitative advantages of the intact-tissue approach were demonstrated compared to conventional thin-section histology, pointing to broad applications in both research and clinical settings.Brian HsuehVanessa M. BurnsPhilip PauersteinKatherine HolzemLi YeKristin EngbergAi-Chi WangXueying GuHarini ChakravarthyH. Efsun ArdaGregory CharvilleHannes VogelIgor R. EfimovSeung KimKarl DeisserothNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brian Hsueh Vanessa M. Burns Philip Pauerstein Katherine Holzem Li Ye Kristin Engberg Ai-Chi Wang Xueying Gu Harini Chakravarthy H. Efsun Arda Gregory Charville Hannes Vogel Igor R. Efimov Seung Kim Karl Deisseroth Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease
description	Abstract Three-dimensional tissue-structural relationships are not well captured by typical thin-section histology, posing challenges for the study of tissue physiology and pathology. Moreover, while recent progress has been made with intact methods for clearing, labeling, and imaging whole organs such as the mature brain, these approaches are generally unsuitable for soft, irregular, and heterogeneous tissues that account for the vast majority of clinical samples and biopsies. Here we develop a biphasic hydrogel methodology, which along with automated analysis, provides for high-throughput quantitative volumetric interrogation of spatially-irregular and friable tissue structures. We validate and apply this approach in the examination of a variety of developing and diseased tissues, with specific focus on the dynamics of normal and pathological pancreatic innervation and development, including in clinical samples. Quantitative advantages of the intact-tissue approach were demonstrated compared to conventional thin-section histology, pointing to broad applications in both research and clinical settings.
format	article
author	Brian Hsueh Vanessa M. Burns Philip Pauerstein Katherine Holzem Li Ye Kristin Engberg Ai-Chi Wang Xueying Gu Harini Chakravarthy H. Efsun Arda Gregory Charville Hannes Vogel Igor R. Efimov Seung Kim Karl Deisseroth
author_facet	Brian Hsueh Vanessa M. Burns Philip Pauerstein Katherine Holzem Li Ye Kristin Engberg Ai-Chi Wang Xueying Gu Harini Chakravarthy H. Efsun Arda Gregory Charville Hannes Vogel Igor R. Efimov Seung Kim Karl Deisseroth
author_sort	Brian Hsueh
title	Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease
title_short	Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease
title_full	Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease
title_fullStr	Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease
title_full_unstemmed	Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease
title_sort	pathways to clinical clarity: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b005b26d2af84e9196f1657c71463209
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Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease

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