Clustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.

Lesions in the DNA arise under ionizing irradiation conditions or various chemical oxidants as a single damage or as part of a multiply damaged site within 1-2 helical turns (clustered lesion). Here, we explored the repair opportunity of the apurinic/apyrimidinic site (AP site) composed of the clust...

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Autores principales:	Ekaterina A Belousova, Inna A Vasil'eva, Nina A Moor, Timofey S Zatsepin, Tatiana S Oretskaya, Olga I Lavrik
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b00a447fbb75479c8d6acbe835c3a24d
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spelling	oai:doaj.org-article:b00a447fbb75479c8d6acbe835c3a24d2021-11-18T09:01:04ZClustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.1932-620310.1371/journal.pone.0068576https://doaj.org/article/b00a447fbb75479c8d6acbe835c3a24d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936307/?tool=EBIhttps://doaj.org/toc/1932-6203Lesions in the DNA arise under ionizing irradiation conditions or various chemical oxidants as a single damage or as part of a multiply damaged site within 1-2 helical turns (clustered lesion). Here, we explored the repair opportunity of the apurinic/apyrimidinic site (AP site) composed of the clustered lesion with 5-formyluracil (5-foU) by the base excision repair (BER) proteins. We found, that if the AP site is shifted relative to the 5-foU of the opposite strand, it could be repaired primarily via the short-patch BER pathway. In this case, the cleavage efficiency of the AP site-containing DNA strand catalyzed by human apurinic/apyrimidinic endonuclease 1 (hAPE1) decreased under AP site excursion to the 3'-side relative to the lesion in the other DNA strand. DNA synthesis catalyzed by DNA polymerase lambda was more accurate in comparison to the one catalyzed by DNA polymerase beta. If the AP site was located exactly opposite 5-foU it was expected to switch the repair to the long-patch BER pathway. In this situation, human processivity factor hPCNA stimulates the process.Ekaterina A BelousovaInna A Vasil'evaNina A MoorTimofey S ZatsepinTatiana S OretskayaOlga I LavrikPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e68576 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ekaterina A Belousova Inna A Vasil'eva Nina A Moor Timofey S Zatsepin Tatiana S Oretskaya Olga I Lavrik Clustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.
description	Lesions in the DNA arise under ionizing irradiation conditions or various chemical oxidants as a single damage or as part of a multiply damaged site within 1-2 helical turns (clustered lesion). Here, we explored the repair opportunity of the apurinic/apyrimidinic site (AP site) composed of the clustered lesion with 5-formyluracil (5-foU) by the base excision repair (BER) proteins. We found, that if the AP site is shifted relative to the 5-foU of the opposite strand, it could be repaired primarily via the short-patch BER pathway. In this case, the cleavage efficiency of the AP site-containing DNA strand catalyzed by human apurinic/apyrimidinic endonuclease 1 (hAPE1) decreased under AP site excursion to the 3'-side relative to the lesion in the other DNA strand. DNA synthesis catalyzed by DNA polymerase lambda was more accurate in comparison to the one catalyzed by DNA polymerase beta. If the AP site was located exactly opposite 5-foU it was expected to switch the repair to the long-patch BER pathway. In this situation, human processivity factor hPCNA stimulates the process.
format	article
author	Ekaterina A Belousova Inna A Vasil'eva Nina A Moor Timofey S Zatsepin Tatiana S Oretskaya Olga I Lavrik
author_facet	Ekaterina A Belousova Inna A Vasil'eva Nina A Moor Timofey S Zatsepin Tatiana S Oretskaya Olga I Lavrik
author_sort	Ekaterina A Belousova
title	Clustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.
title_short	Clustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.
title_full	Clustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.
title_fullStr	Clustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.
title_full_unstemmed	Clustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.
title_sort	clustered dna lesions containing 5-formyluracil and ap site: repair via the ber system.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b00a447fbb75479c8d6acbe835c3a24d
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Clustered DNA lesions containing 5-formyluracil and AP site: repair via the BER system.

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