Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling

Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling

Heat stress (HS) is one of the most serious factors to negatively affect the lactation performance of dairy cows. Bovine mammary epithelial cells are important for lactation. It was demonstrated that HS decreases the lactation performance of dairy cows, partly through altering gene expression within...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lin Fu, Li Zhang, Li Liu, Heng Yang, Peng Zhou, Fan Song, Guozhong Dong, Juncai Chen, Gaofu Wang, Xianwen Dong
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
heat stress
metabolomics
amino acid metabolism
milk protein synthesis
MAC-T cell
Veterinary medicine
SF600-1100
Zoology
QL1-991
Acceso en línea:https://doaj.org/article/b00f202a557647df868dfb6496271ed0
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:b00f202a557647df868dfb6496271ed0
record_format dspace
spelling oai:doaj.org-article:b00f202a557647df868dfb6496271ed02021-11-25T16:17:17ZEffect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling10.3390/ani111131532076-2615https://doaj.org/article/b00f202a557647df868dfb6496271ed02021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3153https://doaj.org/toc/2076-2615Heat stress (HS) is one of the most serious factors to negatively affect the lactation performance of dairy cows. Bovine mammary epithelial cells are important for lactation. It was demonstrated that HS decreases the lactation performance of dairy cows, partly through altering gene expression within bovine mammary epithelial tissue. However, the cellular metabolism mechanisms under HS remains largely unknown. The objective of this study was to determine whether HS induced changes in intracellular metabolites and gene transcription related to amino acid metabolism, amino acid transportation and the mTOR signaling pathway. Immortalized bovine mammary epithelial cell lines (MAC-T cells, <i>n</i> = 5 replicates/treatment) were incubated for 12 h at 37 °C (Control group) and 42 °C (HS group). Relative to the control group, HS led to a greater mRNA expression of heat shock protein genes <i>HSF1, HSPB8, HSPA5, HSP90AB1</i> and <i>HSPA1A</i>. Compared with the control group, metabolomics using liquid chromatography tandem–mass spectrometry identified 417 differential metabolites with <i>p</i> < 0.05 and a variable importance in projection (VIP) score >1.0 in the HS group. HS resulted in significant changes to the intracellular amino acid metabolism of glutathione, phenylalanine, tyrosine, tryptophan, valine, leucine, isoleucine, arginine, proline, cysteine, methionine, alanine, aspartate and glutamate. HS led to a greater mRNA expression of the amino acid transporter genes <i>SLC43A1</i>, <i>SLC38A9</i>, <i>SLC36A1</i>, and <i>SLC3A2</i> but a lower mRNA expression of <i>SLC7A5</i> and <i>SLC38A2</i>. Additionally, HS influenced the expression of genes associated with the mTOR signaling pathway and significantly upregulated the mRNA expression of mTOR, <i>AKT</i>, <i>RHEB</i>, <i>eIF4E</i> and <i>eEF2K</i> but decreased the mRNA expression of <i>TSC1, TSC2</i> and <i>eEF2</i> relative to the control group. Compared with the control group, HS also led to greater mRNA expression of the <i>CSN1S2</i> gene. Overall, our study indicates that bovine mammary epithelial cells may have the ability to resist HS damage and continue milk protein synthesis partly through enhanced intracellular amino acid absorption and metabolism and by activating the mTOR signaling pathway during HS.Lin FuLi ZhangLi LiuHeng YangPeng ZhouFan SongGuozhong DongJuncai ChenGaofu WangXianwen DongMDPI AGarticleheat stressmetabolomicsamino acid metabolismmilk protein synthesisMAC-T cellVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3153, p 3153 (2021)
institution DOAJ
collection DOAJ
language EN
topic heat stress
metabolomics
amino acid metabolism
milk protein synthesis
MAC-T cell
Veterinary medicine
SF600-1100
Zoology
QL1-991
spellingShingle heat stress
metabolomics
amino acid metabolism
milk protein synthesis
MAC-T cell
Veterinary medicine
SF600-1100
Zoology
QL1-991
Lin Fu
Li Zhang
Li Liu
Heng Yang
Peng Zhou
Fan Song
Guozhong Dong
Juncai Chen
Gaofu Wang
Xianwen Dong
Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling
description Heat stress (HS) is one of the most serious factors to negatively affect the lactation performance of dairy cows. Bovine mammary epithelial cells are important for lactation. It was demonstrated that HS decreases the lactation performance of dairy cows, partly through altering gene expression within bovine mammary epithelial tissue. However, the cellular metabolism mechanisms under HS remains largely unknown. The objective of this study was to determine whether HS induced changes in intracellular metabolites and gene transcription related to amino acid metabolism, amino acid transportation and the mTOR signaling pathway. Immortalized bovine mammary epithelial cell lines (MAC-T cells, <i>n</i> = 5 replicates/treatment) were incubated for 12 h at 37 °C (Control group) and 42 °C (HS group). Relative to the control group, HS led to a greater mRNA expression of heat shock protein genes <i>HSF1, HSPB8, HSPA5, HSP90AB1</i> and <i>HSPA1A</i>. Compared with the control group, metabolomics using liquid chromatography tandem–mass spectrometry identified 417 differential metabolites with <i>p</i> < 0.05 and a variable importance in projection (VIP) score >1.0 in the HS group. HS resulted in significant changes to the intracellular amino acid metabolism of glutathione, phenylalanine, tyrosine, tryptophan, valine, leucine, isoleucine, arginine, proline, cysteine, methionine, alanine, aspartate and glutamate. HS led to a greater mRNA expression of the amino acid transporter genes <i>SLC43A1</i>, <i>SLC38A9</i>, <i>SLC36A1</i>, and <i>SLC3A2</i> but a lower mRNA expression of <i>SLC7A5</i> and <i>SLC38A2</i>. Additionally, HS influenced the expression of genes associated with the mTOR signaling pathway and significantly upregulated the mRNA expression of mTOR, <i>AKT</i>, <i>RHEB</i>, <i>eIF4E</i> and <i>eEF2K</i> but decreased the mRNA expression of <i>TSC1, TSC2</i> and <i>eEF2</i> relative to the control group. Compared with the control group, HS also led to greater mRNA expression of the <i>CSN1S2</i> gene. Overall, our study indicates that bovine mammary epithelial cells may have the ability to resist HS damage and continue milk protein synthesis partly through enhanced intracellular amino acid absorption and metabolism and by activating the mTOR signaling pathway during HS.
format article
author Lin Fu
Li Zhang
Li Liu
Heng Yang
Peng Zhou
Fan Song
Guozhong Dong
Juncai Chen
Gaofu Wang
Xianwen Dong
author_facet Lin Fu
Li Zhang
Li Liu
Heng Yang
Peng Zhou
Fan Song
Guozhong Dong
Juncai Chen
Gaofu Wang
Xianwen Dong
author_sort Lin Fu
title Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling
title_short Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling
title_full Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling
title_fullStr Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling
title_full_unstemmed Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling
title_sort effect of heat stress on bovine mammary cellular metabolites and gene transcription related to amino acid metabolism, amino acid transportation and mammalian target of rapamycin (mtor) signaling
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/b00f202a557647df868dfb6496271ed0
work_keys_str_mv AT linfu effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT lizhang effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT liliu effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT hengyang effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT pengzhou effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT fansong effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT guozhongdong effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT juncaichen effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT gaofuwang effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
AT xianwendong effectofheatstressonbovinemammarycellularmetabolitesandgenetranscriptionrelatedtoaminoacidmetabolismaminoacidtransportationandmammaliantargetofrapamycinmtorsignaling
_version_ 1718413277592027136

Ejemplares similares