LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α

Cardiomyocyte apoptosis and autophagy play important roles in acute myocardial infarction (AMI), but the effect of leucine-rich alpha-2-glycoprotein 1 (LRG1) on the apoptosis and autophagy of H9c2 has not yet been reported. It was found through differential gene analysis and LASSO analysis that LRG1...

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Autores principales:	Jiajie Feng, Jiachen Zhan, Shuangshuang Ma
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	lrg1 hif-1α acute myocardial infarction autophagy apoptosis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/b0113b56390b4ff1b9a500c33f3e811f
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spelling	oai:doaj.org-article:b0113b56390b4ff1b9a500c33f3e811f2021-11-04T15:51:53ZLRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α2165-59792165-598710.1080/21655979.2021.1988368https://doaj.org/article/b0113b56390b4ff1b9a500c33f3e811f2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1988368https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Cardiomyocyte apoptosis and autophagy play important roles in acute myocardial infarction (AMI), but the effect of leucine-rich alpha-2-glycoprotein 1 (LRG1) on the apoptosis and autophagy of H9c2 has not yet been reported. It was found through differential gene analysis and LASSO analysis that LRG1 was the key gene in AMI. In this study, western blot was applied to detect the protein expression of Bax, Bcl2, LC3, p62, LRG1 and hypoxia-inducible factor-1α (HIF-1α); CCK-8 assay was employed to detect cell viability; Annexin V-FITC/PI staining was adopted to evaluate apoptosis, and immunofluorescence assay was applied to detect autophagy. Under hypoxia conditions in H9c2 cells, LRG1 protein levels were increased, the cell activity was decreased, and apoptosis and autophagy were promoted; the downregulated LRG1 significantly enhanced cell viability but inhibited apoptosis and autophagy. When knocking down HIF-1α in the overexpressed LRG1 cells, the effects of LRG1 were reversed under hypoxia condition. In conclusion, LRG1/HIF-1α promoted H9c2 cell apoptosis and autophagy in hypoxia, potentially providing new ideas for the determination and treatment of AMI. Abbreviation: LRG1: Leucine-rich alpha-2-glycoprotein 1; LRR: leucine-rich repeat; HIF-1α: Hypoxia-inducible factor-1α; AMI: acute myocardial infarctionJiajie FengJiachen ZhanShuangshuang MaTaylor & Francis Grouparticlelrg1hif-1αacute myocardial infarctionautophagyapoptosisBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8897-8907 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lrg1 hif-1α acute myocardial infarction autophagy apoptosis Biotechnology TP248.13-248.65
spellingShingle	lrg1 hif-1α acute myocardial infarction autophagy apoptosis Biotechnology TP248.13-248.65 Jiajie Feng Jiachen Zhan Shuangshuang Ma LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α
description	Cardiomyocyte apoptosis and autophagy play important roles in acute myocardial infarction (AMI), but the effect of leucine-rich alpha-2-glycoprotein 1 (LRG1) on the apoptosis and autophagy of H9c2 has not yet been reported. It was found through differential gene analysis and LASSO analysis that LRG1 was the key gene in AMI. In this study, western blot was applied to detect the protein expression of Bax, Bcl2, LC3, p62, LRG1 and hypoxia-inducible factor-1α (HIF-1α); CCK-8 assay was employed to detect cell viability; Annexin V-FITC/PI staining was adopted to evaluate apoptosis, and immunofluorescence assay was applied to detect autophagy. Under hypoxia conditions in H9c2 cells, LRG1 protein levels were increased, the cell activity was decreased, and apoptosis and autophagy were promoted; the downregulated LRG1 significantly enhanced cell viability but inhibited apoptosis and autophagy. When knocking down HIF-1α in the overexpressed LRG1 cells, the effects of LRG1 were reversed under hypoxia condition. In conclusion, LRG1/HIF-1α promoted H9c2 cell apoptosis and autophagy in hypoxia, potentially providing new ideas for the determination and treatment of AMI. Abbreviation: LRG1: Leucine-rich alpha-2-glycoprotein 1; LRR: leucine-rich repeat; HIF-1α: Hypoxia-inducible factor-1α; AMI: acute myocardial infarction
format	article
author	Jiajie Feng Jiachen Zhan Shuangshuang Ma
author_facet	Jiajie Feng Jiachen Zhan Shuangshuang Ma
author_sort	Jiajie Feng
title	LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α
title_short	LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α
title_full	LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α
title_fullStr	LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α
title_full_unstemmed	LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α
title_sort	lrg1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/b0113b56390b4ff1b9a500c33f3e811f
work_keys_str_mv	AT jiajiefeng lrg1promoteshypoxiainducedcardiomyocyteapoptosisandautophagybyregulatinghypoxiainduciblefactor1a AT jiachenzhan lrg1promoteshypoxiainducedcardiomyocyteapoptosisandautophagybyregulatinghypoxiainduciblefactor1a AT shuangshuangma lrg1promoteshypoxiainducedcardiomyocyteapoptosisandautophagybyregulatinghypoxiainduciblefactor1a
_version_	1718444734496636928

LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α

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