Purification of human serum immunoglobulins by Thiophilic chromatography (T-gel)

Background and Objective: Human-derived antibodies, which are prepared by expensive methods, are used in diagnosis, prophylaxis, and treatment of diseases. The aim of this study was to develop an effective and inexpensive method for purification of human serum immunoglobulins. Methods: Human serum i...

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Autores principales:	M Pour Amir, S Asadallahi Dabagh, B Pak Nejad
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2002
Materias:	purification immunoglobulins human serum thiophilic gel Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b0190606c7374e718fb7ddc01ae1ac3d
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spelling	oai:doaj.org-article:b0190606c7374e718fb7ddc01ae1ac3d2021-11-10T09:19:40ZPurification of human serum immunoglobulins by Thiophilic chromatography (T-gel)1561-41072251-7170https://doaj.org/article/b0190606c7374e718fb7ddc01ae1ac3d2002-07-01T00:00:00Zhttp://jbums.org/article-1-2816-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Background and Objective: Human-derived antibodies, which are prepared by expensive methods, are used in diagnosis, prophylaxis, and treatment of diseases. The aim of this study was to develop an effective and inexpensive method for purification of human serum immunoglobulins. Methods: Human serum immunoglobulins were precipitated by ammonium sulfate (50% saturation) and then purification was done by Thiophilic chromatography (T-gel). Purification of antibodies was evaluated by cellulose acetate electrophoresis. Concentration of antibodies (IgG, IgM, IgA) in serum and the resulting pooled fractions were estimated by single radial immunodiffusion (SRID) and percentage of recovery was calculated. Findings: Analysis of products by electrophoresis showed only one band in γ-region. Recovery of the purified IgG was 75% while that for IgM was considerably low (about 17%). Conclusion: The purified immunoglobulins were eluated by a low salt buffer closely neutral pH, thus post treatment of product was not necessary. Thiophilic chromatography causes the purification of immunoglobulins at a stage after ammonium sulfate precipitation. The purified immunoglobulins in this study could be suitable for immunochemistry methods.M Pour AmirS Asadallahi DabaghB Pak NejadBabol University of Medical Sciencesarticlepurificationimmunoglobulinshuman serumthiophilic gelMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 4, Iss 3, Pp 12-17 (2002)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	purification immunoglobulins human serum thiophilic gel Medicine R Medicine (General) R5-920
spellingShingle	purification immunoglobulins human serum thiophilic gel Medicine R Medicine (General) R5-920 M Pour Amir S Asadallahi Dabagh B Pak Nejad Purification of human serum immunoglobulins by Thiophilic chromatography (T-gel)
description	Background and Objective: Human-derived antibodies, which are prepared by expensive methods, are used in diagnosis, prophylaxis, and treatment of diseases. The aim of this study was to develop an effective and inexpensive method for purification of human serum immunoglobulins. Methods: Human serum immunoglobulins were precipitated by ammonium sulfate (50% saturation) and then purification was done by Thiophilic chromatography (T-gel). Purification of antibodies was evaluated by cellulose acetate electrophoresis. Concentration of antibodies (IgG, IgM, IgA) in serum and the resulting pooled fractions were estimated by single radial immunodiffusion (SRID) and percentage of recovery was calculated. Findings: Analysis of products by electrophoresis showed only one band in γ-region. Recovery of the purified IgG was 75% while that for IgM was considerably low (about 17%). Conclusion: The purified immunoglobulins were eluated by a low salt buffer closely neutral pH, thus post treatment of product was not necessary. Thiophilic chromatography causes the purification of immunoglobulins at a stage after ammonium sulfate precipitation. The purified immunoglobulins in this study could be suitable for immunochemistry methods.
format	article
author	M Pour Amir S Asadallahi Dabagh B Pak Nejad
author_facet	M Pour Amir S Asadallahi Dabagh B Pak Nejad
author_sort	M Pour Amir
title	Purification of human serum immunoglobulins by Thiophilic chromatography (T-gel)
title_short	Purification of human serum immunoglobulins by Thiophilic chromatography (T-gel)
title_full	Purification of human serum immunoglobulins by Thiophilic chromatography (T-gel)
title_fullStr	Purification of human serum immunoglobulins by Thiophilic chromatography (T-gel)
title_full_unstemmed	Purification of human serum immunoglobulins by Thiophilic chromatography (T-gel)
title_sort	purification of human serum immunoglobulins by thiophilic chromatography (t-gel)
publisher	Babol University of Medical Sciences
publishDate	2002
url	https://doaj.org/article/b0190606c7374e718fb7ddc01ae1ac3d
work_keys_str_mv	AT mpouramir purificationofhumanserumimmunoglobulinsbythiophilicchromatographytgel AT sasadallahidabagh purificationofhumanserumimmunoglobulinsbythiophilicchromatographytgel AT bpaknejad purificationofhumanserumimmunoglobulinsbythiophilicchromatographytgel
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Purification of human serum immunoglobulins by Thiophilic chromatography (T-gel)

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