Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma

Xiaoju Zhou,1,2,* Mengzi Zhang,2,* Bryant Yung,2 Hong Li,2 Chenguang Zhou,2 L James Lee,3,4 Robert J Lee2,41State Key Laboratory of Virology, Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University School of Pharmaceutical Sciences, Wuhan, People&a...

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Autores principales:	Zhou X, Zhang M, Yung B, Li H, Zhou C, Lee LJ, Lee RJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b01fc40554ad48549e12d2312bb67efa
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spelling	oai:doaj.org-article:b01fc40554ad48549e12d2312bb67efa2021-12-02T05:56:08ZLactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma1176-91141178-2013https://doaj.org/article/b01fc40554ad48549e12d2312bb67efa2012-10-01T00:00:00Zhttp://www.dovepress.com/lactosylated-liposomes-for-targeted-delivery-of-doxorubicin-to-hepatoc-a11277https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xiaoju Zhou,1,2,* Mengzi Zhang,2,* Bryant Yung,2 Hong Li,2 Chenguang Zhou,2 L James Lee,3,4 Robert J Lee2,41State Key Laboratory of Virology, Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University School of Pharmaceutical Sciences, Wuhan, People&rsquo;s Republic of China; 2Division of Pharmaceutics, 3Department of Chemical and Biomolecular Engineering, 4NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, OH, USA*These authors contributed equally to this workBackground: N-lactosyl-dioleoylphosphatidylethanolamine (Lac-DOPE) was synthesized and evaluated as a liver-specific targeting ligand via asialoglycoprotein receptors for liposomal delivery of doxorubicin.Methods: Lactosylated liposomes encapsulating calcein (Lac-L-calcein) or doxorubicin (Lac-L-DOX) composed of egg phosphatidylcholine, cholesterol, monomethoxy polyethylene glycol 2000-distearoyl phosphatidylethanolamine, and Lac-DOPE at 50:35:5:10 (mol/mol) were prepared by polycarbonate membrane extrusion and evaluated in human hepatocellular carcinoma HepG2 cells. Cellular uptake of Lac-L-calcein was monitored by confocal microscopy and by flow cytometry. The cytotoxicity of Lac-L-DOX was evaluated by MTT assay. The pharmacokinetic properties of Lac-L-DOX were studied in normal mice, and its biodistribution and antitumor activity were studied in nude mice with HepG2 xenografts.Results: The size of Lac-L-DOX was less than 100 nm and the liposomes demonstrated excellent colloidal stability. In vitro uptake of Lac-L-calcein by HepG2 cells was four times greater than that of non-targeted L-calcein. In the presence of 20 mM lactose, the uptake of Lac-L-calcein was inhibited, suggesting that asialoglycoprotein receptors mediated the observed cellular uptake. Lac-L-DOX exhibited enhanced in vivo cytotoxicity compared with the nontargeted liposomal doxorubicin (L-DOX), and its pharmacokinetic parameters indicate that Lac-L-DOX has a long blood circulation time (t1/2 8.73 hours). Tissue distribution and therapeutic efficacy studies in nude mice bearing HepG2 xenografts show that Lac-L-DOX had significantly stronger tumor inhibitory activity compared with L-DOX and free doxorubicin, along with a higher accumulation of drug within the tumor site and greater cellular uptake by tumor cells.Conclusion: These data suggest that lactosylated liposomes are promising drug delivery vehicles for hepatocellular carcinoma.Keywords: liposomes, hepatocellular carcinoma, asialoglycoprotein receptor, drug targetingZhou XZhang MYung BLi HZhou CLee LJLee RJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5465-5474 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhou X Zhang M Yung B Li H Zhou C Lee LJ Lee RJ Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma
description	Xiaoju Zhou,1,2,* Mengzi Zhang,2,* Bryant Yung,2 Hong Li,2 Chenguang Zhou,2 L James Lee,3,4 Robert J Lee2,41State Key Laboratory of Virology, Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University School of Pharmaceutical Sciences, Wuhan, People&rsquo;s Republic of China; 2Division of Pharmaceutics, 3Department of Chemical and Biomolecular Engineering, 4NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, OH, USA*These authors contributed equally to this workBackground: N-lactosyl-dioleoylphosphatidylethanolamine (Lac-DOPE) was synthesized and evaluated as a liver-specific targeting ligand via asialoglycoprotein receptors for liposomal delivery of doxorubicin.Methods: Lactosylated liposomes encapsulating calcein (Lac-L-calcein) or doxorubicin (Lac-L-DOX) composed of egg phosphatidylcholine, cholesterol, monomethoxy polyethylene glycol 2000-distearoyl phosphatidylethanolamine, and Lac-DOPE at 50:35:5:10 (mol/mol) were prepared by polycarbonate membrane extrusion and evaluated in human hepatocellular carcinoma HepG2 cells. Cellular uptake of Lac-L-calcein was monitored by confocal microscopy and by flow cytometry. The cytotoxicity of Lac-L-DOX was evaluated by MTT assay. The pharmacokinetic properties of Lac-L-DOX were studied in normal mice, and its biodistribution and antitumor activity were studied in nude mice with HepG2 xenografts.Results: The size of Lac-L-DOX was less than 100 nm and the liposomes demonstrated excellent colloidal stability. In vitro uptake of Lac-L-calcein by HepG2 cells was four times greater than that of non-targeted L-calcein. In the presence of 20 mM lactose, the uptake of Lac-L-calcein was inhibited, suggesting that asialoglycoprotein receptors mediated the observed cellular uptake. Lac-L-DOX exhibited enhanced in vivo cytotoxicity compared with the nontargeted liposomal doxorubicin (L-DOX), and its pharmacokinetic parameters indicate that Lac-L-DOX has a long blood circulation time (t1/2 8.73 hours). Tissue distribution and therapeutic efficacy studies in nude mice bearing HepG2 xenografts show that Lac-L-DOX had significantly stronger tumor inhibitory activity compared with L-DOX and free doxorubicin, along with a higher accumulation of drug within the tumor site and greater cellular uptake by tumor cells.Conclusion: These data suggest that lactosylated liposomes are promising drug delivery vehicles for hepatocellular carcinoma.Keywords: liposomes, hepatocellular carcinoma, asialoglycoprotein receptor, drug targeting
format	article
author	Zhou X Zhang M Yung B Li H Zhou C Lee LJ Lee RJ
author_facet	Zhou X Zhang M Yung B Li H Zhou C Lee LJ Lee RJ
author_sort	Zhou X
title	Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma
title_short	Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma
title_full	Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma
title_fullStr	Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma
title_full_unstemmed	Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma
title_sort	lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/b01fc40554ad48549e12d2312bb67efa
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Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma

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