Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.

Team1 (vB_SauM_Team1) is a polyvalent staphylococcal phage belonging to the Myoviridae family. Phage Team1 was propagated on a Staphylococcus aureus strain and a non-pathogenic Staphylococcus xylosus strain used in industrial meat fermentation. The two Team1 preparations were compared with respect t...

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Autores principales:	Lynn El Haddad, Nour Ben Abdallah, Pier-Luc Plante, Jeannot Dumaresq, Ramaz Katsarava, Steve Labrie, Jacques Corbeil, Daniel St-Gelais, Sylvain Moineau
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b022b5a07aa249a48230bd9dcae68530
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spelling	oai:doaj.org-article:b022b5a07aa249a48230bd9dcae685302021-11-25T06:07:09ZImproving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.1932-620310.1371/journal.pone.0102600https://doaj.org/article/b022b5a07aa249a48230bd9dcae685302014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25061757/?tool=EBIhttps://doaj.org/toc/1932-6203Team1 (vB_SauM_Team1) is a polyvalent staphylococcal phage belonging to the Myoviridae family. Phage Team1 was propagated on a Staphylococcus aureus strain and a non-pathogenic Staphylococcus xylosus strain used in industrial meat fermentation. The two Team1 preparations were compared with respect to their microbiological and genomic properties. The burst sizes, latent periods, and host ranges of the two derivatives were identical as were their genome sequences. Phage Team1 has 140,903 bp of double stranded DNA encoding for 217 open reading frames and 4 tRNAs. Comparative genomic analysis revealed similarities to staphylococcal phages ISP (97%) and G1 (97%). The host range of Team1 was compared to the well-known polyvalent staphylococcal phages phi812 and K using a panel of 57 S. aureus strains collected from various sources. These bacterial strains were found to represent 18 sequence types (MLST) and 14 clonal complexes (eBURST). Altogether, the three phages propagated on S. xylosus lysed 52 out of 57 distinct strains of S. aureus. The identification of phage-insensitive strains underlines the importance of designing phage cocktails with broadly varying and overlapping host ranges. Taken altogether, our study suggests that some staphylococcal phages can be propagated on food-grade bacteria for biocontrol and safety purposes.Lynn El HaddadNour Ben AbdallahPier-Luc PlanteJeannot DumaresqRamaz KatsaravaSteve LabrieJacques CorbeilDaniel St-GelaisSylvain MoineauPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102600 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lynn El Haddad Nour Ben Abdallah Pier-Luc Plante Jeannot Dumaresq Ramaz Katsarava Steve Labrie Jacques Corbeil Daniel St-Gelais Sylvain Moineau Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.
description	Team1 (vB_SauM_Team1) is a polyvalent staphylococcal phage belonging to the Myoviridae family. Phage Team1 was propagated on a Staphylococcus aureus strain and a non-pathogenic Staphylococcus xylosus strain used in industrial meat fermentation. The two Team1 preparations were compared with respect to their microbiological and genomic properties. The burst sizes, latent periods, and host ranges of the two derivatives were identical as were their genome sequences. Phage Team1 has 140,903 bp of double stranded DNA encoding for 217 open reading frames and 4 tRNAs. Comparative genomic analysis revealed similarities to staphylococcal phages ISP (97%) and G1 (97%). The host range of Team1 was compared to the well-known polyvalent staphylococcal phages phi812 and K using a panel of 57 S. aureus strains collected from various sources. These bacterial strains were found to represent 18 sequence types (MLST) and 14 clonal complexes (eBURST). Altogether, the three phages propagated on S. xylosus lysed 52 out of 57 distinct strains of S. aureus. The identification of phage-insensitive strains underlines the importance of designing phage cocktails with broadly varying and overlapping host ranges. Taken altogether, our study suggests that some staphylococcal phages can be propagated on food-grade bacteria for biocontrol and safety purposes.
format	article
author	Lynn El Haddad Nour Ben Abdallah Pier-Luc Plante Jeannot Dumaresq Ramaz Katsarava Steve Labrie Jacques Corbeil Daniel St-Gelais Sylvain Moineau
author_facet	Lynn El Haddad Nour Ben Abdallah Pier-Luc Plante Jeannot Dumaresq Ramaz Katsarava Steve Labrie Jacques Corbeil Daniel St-Gelais Sylvain Moineau
author_sort	Lynn El Haddad
title	Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.
title_short	Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.
title_full	Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.
title_fullStr	Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.
title_full_unstemmed	Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.
title_sort	improving the safety of staphylococcus aureus polyvalent phages by their production on a staphylococcus xylosus strain.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b022b5a07aa249a48230bd9dcae68530
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Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain.

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