MicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.

MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study, we further demonstrated that...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu Wang, Jianwei Ren, Yun Gao, Joel Z I Ma, Han Chong Toh, Pierce Chow, Alexander Y F Chung, London L P J Ooi, Caroline G L Lee
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b02347caf191495fa33d49eaff6e98a5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b02347caf191495fa33d49eaff6e98a5
record_format	dspace
spelling	oai:doaj.org-article:b02347caf191495fa33d49eaff6e98a52021-11-18T09:02:30ZMicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.1932-620310.1371/journal.pone.0068744https://doaj.org/article/b02347caf191495fa33d49eaff6e98a52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23922662/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study, we further demonstrated that miR-224 up-regulation promotes cell proliferation using both in vitro assays and in vivo tumor growth models. We systematically screened for high confidence miR-224 targets by overlapping in silico predicted targets from multiple algorithms and significantly down-regulated genes in miR-224-expressing cells from whole genome expression microarrays. A total of 72 high confidence miR-224 targets were identified and found to be enriched in various cancer-related processes. SMAD family member 4 (SMAD4) is experimentally validated as the direct cellular target through which miR-224 promotes cell proliferation. The clinical relevance of our experimental observations was supported by a statistically significant inverse correlation between miR-224 and SMAD4 transcript expression in tumor versus paired adjacent non-tumorous tissues from HCC patients (p<0.001, r= -0.45, R(2) =0.122). Furthermore, miR-224 up-regulation and SMAD4 down-regulation is significantly associated with poorer patient survival (p<0.05). In summary, miR-224/SMAD4 pathway is a clinically relevant pathway to provide new insights in understanding HCC. (191 words).Yu WangJianwei RenYun GaoJoel Z I MaHan Chong TohPierce ChowAlexander Y F ChungLondon L P J OoiCaroline G L LeePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68744 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu Wang Jianwei Ren Yun Gao Joel Z I Ma Han Chong Toh Pierce Chow Alexander Y F Chung London L P J Ooi Caroline G L Lee MicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.
description	MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study, we further demonstrated that miR-224 up-regulation promotes cell proliferation using both in vitro assays and in vivo tumor growth models. We systematically screened for high confidence miR-224 targets by overlapping in silico predicted targets from multiple algorithms and significantly down-regulated genes in miR-224-expressing cells from whole genome expression microarrays. A total of 72 high confidence miR-224 targets were identified and found to be enriched in various cancer-related processes. SMAD family member 4 (SMAD4) is experimentally validated as the direct cellular target through which miR-224 promotes cell proliferation. The clinical relevance of our experimental observations was supported by a statistically significant inverse correlation between miR-224 and SMAD4 transcript expression in tumor versus paired adjacent non-tumorous tissues from HCC patients (p<0.001, r= -0.45, R(2) =0.122). Furthermore, miR-224 up-regulation and SMAD4 down-regulation is significantly associated with poorer patient survival (p<0.05). In summary, miR-224/SMAD4 pathway is a clinically relevant pathway to provide new insights in understanding HCC. (191 words).
format	article
author	Yu Wang Jianwei Ren Yun Gao Joel Z I Ma Han Chong Toh Pierce Chow Alexander Y F Chung London L P J Ooi Caroline G L Lee
author_facet	Yu Wang Jianwei Ren Yun Gao Joel Z I Ma Han Chong Toh Pierce Chow Alexander Y F Chung London L P J Ooi Caroline G L Lee
author_sort	Yu Wang
title	MicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.
title_short	MicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.
title_full	MicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.
title_fullStr	MicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.
title_full_unstemmed	MicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.
title_sort	microrna-224 targets smad family member 4 to promote cell proliferation and negatively influence patient survival.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b02347caf191495fa33d49eaff6e98a5
work_keys_str_mv	AT yuwang microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival AT jianweiren microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival AT yungao microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival AT joelzima microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival AT hanchongtoh microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival AT piercechow microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival AT alexanderyfchung microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival AT londonlpjooi microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival AT carolinegllee microrna224targetssmadfamilymember4topromotecellproliferationandnegativelyinfluencepatientsurvival
_version_	1718420985028280320

MicroRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival.

Ejemplares similares