Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids

Abstract Aberrant miRNA expression has been associated with many diseases, and extracellular miRNAs that circulate in the bloodstream are remarkably stable. Recently, there has been growing interest in identifying cell-free circulating miRNAs that can serve as non-invasive biomarkers for early detec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lewis Z. Hong, Lihan Zhou, Ruiyang Zou, Chin Meng Khoo, Adeline Lai San Chew, Chih-Liang Chin, Shian-Jiun Shih
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0268cd117244f9d8a2a748be174f675
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b0268cd117244f9d8a2a748be174f675
record_format	dspace
spelling	oai:doaj.org-article:b0268cd117244f9d8a2a748be174f6752021-12-02T13:20:04ZSystematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids10.1038/s41598-021-83365-z2045-2322https://doaj.org/article/b0268cd117244f9d8a2a748be174f6752021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83365-zhttps://doaj.org/toc/2045-2322Abstract Aberrant miRNA expression has been associated with many diseases, and extracellular miRNAs that circulate in the bloodstream are remarkably stable. Recently, there has been growing interest in identifying cell-free circulating miRNAs that can serve as non-invasive biomarkers for early detection of disease or selection of treatment options. However, quantifying miRNA levels in biofluids is technically challenging due to their low abundance. Using reference samples, we performed a cross-platform evaluation in which miRNA profiling was performed on four different qPCR platforms (MiRXES, Qiagen, Applied Biosystems, Exiqon), nCounter technology (NanoString), and miRNA-Seq. Overall, our results suggest that using miRNA-Seq for discovery and targeted qPCR for validation is a rational strategy for miRNA biomarker development in clinical samples that involve limited amounts of biofluids.Lewis Z. HongLihan ZhouRuiyang ZouChin Meng KhooAdeline Lai San ChewChih-Liang ChinShian-Jiun ShihNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lewis Z. Hong Lihan Zhou Ruiyang Zou Chin Meng Khoo Adeline Lai San Chew Chih-Liang Chin Shian-Jiun Shih Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids
description	Abstract Aberrant miRNA expression has been associated with many diseases, and extracellular miRNAs that circulate in the bloodstream are remarkably stable. Recently, there has been growing interest in identifying cell-free circulating miRNAs that can serve as non-invasive biomarkers for early detection of disease or selection of treatment options. However, quantifying miRNA levels in biofluids is technically challenging due to their low abundance. Using reference samples, we performed a cross-platform evaluation in which miRNA profiling was performed on four different qPCR platforms (MiRXES, Qiagen, Applied Biosystems, Exiqon), nCounter technology (NanoString), and miRNA-Seq. Overall, our results suggest that using miRNA-Seq for discovery and targeted qPCR for validation is a rational strategy for miRNA biomarker development in clinical samples that involve limited amounts of biofluids.
format	article
author	Lewis Z. Hong Lihan Zhou Ruiyang Zou Chin Meng Khoo Adeline Lai San Chew Chih-Liang Chin Shian-Jiun Shih
author_facet	Lewis Z. Hong Lihan Zhou Ruiyang Zou Chin Meng Khoo Adeline Lai San Chew Chih-Liang Chin Shian-Jiun Shih
author_sort	Lewis Z. Hong
title	Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids
title_short	Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids
title_full	Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids
title_fullStr	Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids
title_full_unstemmed	Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids
title_sort	systematic evaluation of multiple qpcr platforms, nanostring and mirna-seq for microrna biomarker discovery in human biofluids
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0268cd117244f9d8a2a748be174f675
work_keys_str_mv	AT lewiszhong systematicevaluationofmultipleqpcrplatformsnanostringandmirnaseqformicrornabiomarkerdiscoveryinhumanbiofluids AT lihanzhou systematicevaluationofmultipleqpcrplatformsnanostringandmirnaseqformicrornabiomarkerdiscoveryinhumanbiofluids AT ruiyangzou systematicevaluationofmultipleqpcrplatformsnanostringandmirnaseqformicrornabiomarkerdiscoveryinhumanbiofluids AT chinmengkhoo systematicevaluationofmultipleqpcrplatformsnanostringandmirnaseqformicrornabiomarkerdiscoveryinhumanbiofluids AT adelinelaisanchew systematicevaluationofmultipleqpcrplatformsnanostringandmirnaseqformicrornabiomarkerdiscoveryinhumanbiofluids AT chihliangchin systematicevaluationofmultipleqpcrplatformsnanostringandmirnaseqformicrornabiomarkerdiscoveryinhumanbiofluids AT shianjiunshih systematicevaluationofmultipleqpcrplatformsnanostringandmirnaseqformicrornabiomarkerdiscoveryinhumanbiofluids
_version_	1718393233218732032

Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids

Ejemplares similares