miR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA1

Abstract Post-transcriptional gene regulation by microRNAs (miRNAs) is involved in memory formation. However, the roles of individual miRNAs in these processes remain largely unknown. In this study, we want to clarify the role of miR-181a in hippocampus-dependent memory formation. A transient increa...

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Autores principales:	Sun-fu Zhang, Jun-chen Chen, Jing Zhang, Jian-guo Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b02a2278566840bbbcb15b0b08373b12
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spelling	oai:doaj.org-article:b02a2278566840bbbcb15b0b08373b122021-12-02T12:32:33ZmiR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA110.1038/s41598-017-09095-32045-2322https://doaj.org/article/b02a2278566840bbbcb15b0b08373b122017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09095-3https://doaj.org/toc/2045-2322Abstract Post-transcriptional gene regulation by microRNAs (miRNAs) is involved in memory formation. However, the roles of individual miRNAs in these processes remain largely unknown. In this study, we want to clarify the role of miR-181a in hippocampus-dependent memory formation. A transient increase in miR-181a expression was observed after conditioned fear conditioning (CFC) and object location task (OLT) training. Selective overexpression or inhibition of miR-181a in the dorsal hippocampus (DH) via the injection of a miR-181a agomir or antagomir enhanced or impaired the CFC- and OLT-dependent memory formation, respectively. Using bioinformatics and luciferase assays, we identified PRKAA1 as a potential target gene of miR-181a. After CFC or OLT training, the expression and activity of PRKAA1 decreased as miR-181a expression increased and was effectively blocked by the miR-181a antagomir. Moreover, microinjection of the PRKAA1 agonist AICAR or inhibitor compound C in the DH reversed the roles of the miR-181a agomir or antagomir in CFC- and OLT-dependent memory formation. In conclusion, this work provides novel evidence describing the role and mechanism of miR-181a in hippocampus-dependent memory formation, which sheds light on the potential regulation of cognition and future treatments for cognitive disorders.Sun-fu ZhangJun-chen ChenJing ZhangJian-guo XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sun-fu Zhang Jun-chen Chen Jing Zhang Jian-guo Xu miR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA1
description	Abstract Post-transcriptional gene regulation by microRNAs (miRNAs) is involved in memory formation. However, the roles of individual miRNAs in these processes remain largely unknown. In this study, we want to clarify the role of miR-181a in hippocampus-dependent memory formation. A transient increase in miR-181a expression was observed after conditioned fear conditioning (CFC) and object location task (OLT) training. Selective overexpression or inhibition of miR-181a in the dorsal hippocampus (DH) via the injection of a miR-181a agomir or antagomir enhanced or impaired the CFC- and OLT-dependent memory formation, respectively. Using bioinformatics and luciferase assays, we identified PRKAA1 as a potential target gene of miR-181a. After CFC or OLT training, the expression and activity of PRKAA1 decreased as miR-181a expression increased and was effectively blocked by the miR-181a antagomir. Moreover, microinjection of the PRKAA1 agonist AICAR or inhibitor compound C in the DH reversed the roles of the miR-181a agomir or antagomir in CFC- and OLT-dependent memory formation. In conclusion, this work provides novel evidence describing the role and mechanism of miR-181a in hippocampus-dependent memory formation, which sheds light on the potential regulation of cognition and future treatments for cognitive disorders.
format	article
author	Sun-fu Zhang Jun-chen Chen Jing Zhang Jian-guo Xu
author_facet	Sun-fu Zhang Jun-chen Chen Jing Zhang Jian-guo Xu
author_sort	Sun-fu Zhang
title	miR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA1
title_short	miR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA1
title_full	miR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA1
title_fullStr	miR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA1
title_full_unstemmed	miR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA1
title_sort	mir-181a involves in the hippocampus-dependent memory formation via targeting prkaa1
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b02a2278566840bbbcb15b0b08373b12
work_keys_str_mv	AT sunfuzhang mir181ainvolvesinthehippocampusdependentmemoryformationviatargetingprkaa1 AT junchenchen mir181ainvolvesinthehippocampusdependentmemoryformationviatargetingprkaa1 AT jingzhang mir181ainvolvesinthehippocampusdependentmemoryformationviatargetingprkaa1 AT jianguoxu mir181ainvolvesinthehippocampusdependentmemoryformationviatargetingprkaa1
_version_	1718394055887421440

miR-181a involves in the hippocampus-dependent memory formation via targeting PRKAA1

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