Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.

Recent reports have suggested the involvement of gut microbiota in the progression of colorectal cancer (CRC). We utilized pyrosequencing based analysis of 16S rRNA genes to determine the overall structure of microbiota in patients with colorectal cancer and healthy controls; we investigated microbi...

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Autores principales:	Weiguang Chen, Fanlong Liu, Zongxin Ling, Xiaojuan Tong, Charlie Xiang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b02c07f2e56046899e6d7c1063a93062
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spelling	oai:doaj.org-article:b02c07f2e56046899e6d7c1063a930622021-11-18T07:14:06ZHuman intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.1932-620310.1371/journal.pone.0039743https://doaj.org/article/b02c07f2e56046899e6d7c1063a930622012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22761885/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recent reports have suggested the involvement of gut microbiota in the progression of colorectal cancer (CRC). We utilized pyrosequencing based analysis of 16S rRNA genes to determine the overall structure of microbiota in patients with colorectal cancer and healthy controls; we investigated microbiota of the intestinal lumen, the cancerous tissue and matched noncancerous normal tissue. Moreover, we investigated the mucosa-adherent microbial composition using rectal swab samples because the structure of the tissue-adherent bacterial community is potentially altered following bowel cleansing. Our findings indicated that the microbial structure of the intestinal lumen and cancerous tissue differed significantly. Phylotypes that enhance energy harvest from diets or perform metabolic exchange with the host were more abundant in the lumen. There were more abundant Firmicutes and less abundant Bacteroidetes and Proteobacteria in lumen. The overall microbial structures of cancerous tissue and noncancerous tissue were similar; however the tumor microbiota exhibited lower diversity. The structures of the intestinal lumen microbiota and mucosa-adherent microbiota were different in CRC patients compared to matched microbiota in healthy individuals. Lactobacillales was enriched in cancerous tissue, whereas Faecalibacterium was reduced. In the mucosa-adherent microbiota, Bifidobacterium, Faecalibacterium, and Blautia were reduced in CRC patients, whereas Fusobacterium, Porphyromonas, Peptostreptococcus, and Mogibacterium were enriched. In the lumen, predominant phylotypes related to metabolic disorders or metabolic exchange with the host, Erysipelotrichaceae, Prevotellaceae, and Coriobacteriaceae were increased in cancer patients. Coupled with previous reports, these results suggest that the intestinal microbiota is associated with CRC risk and that intestinal lumen microflora potentially influence CRC risk via cometabolism or metabolic exchange with the host. However, mucosa-associated microbiota potentially affects CRC risk primarily through direct interaction with the host.Weiguang ChenFanlong LiuZongxin LingXiaojuan TongCharlie XiangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39743 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Weiguang Chen Fanlong Liu Zongxin Ling Xiaojuan Tong Charlie Xiang Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
description	Recent reports have suggested the involvement of gut microbiota in the progression of colorectal cancer (CRC). We utilized pyrosequencing based analysis of 16S rRNA genes to determine the overall structure of microbiota in patients with colorectal cancer and healthy controls; we investigated microbiota of the intestinal lumen, the cancerous tissue and matched noncancerous normal tissue. Moreover, we investigated the mucosa-adherent microbial composition using rectal swab samples because the structure of the tissue-adherent bacterial community is potentially altered following bowel cleansing. Our findings indicated that the microbial structure of the intestinal lumen and cancerous tissue differed significantly. Phylotypes that enhance energy harvest from diets or perform metabolic exchange with the host were more abundant in the lumen. There were more abundant Firmicutes and less abundant Bacteroidetes and Proteobacteria in lumen. The overall microbial structures of cancerous tissue and noncancerous tissue were similar; however the tumor microbiota exhibited lower diversity. The structures of the intestinal lumen microbiota and mucosa-adherent microbiota were different in CRC patients compared to matched microbiota in healthy individuals. Lactobacillales was enriched in cancerous tissue, whereas Faecalibacterium was reduced. In the mucosa-adherent microbiota, Bifidobacterium, Faecalibacterium, and Blautia were reduced in CRC patients, whereas Fusobacterium, Porphyromonas, Peptostreptococcus, and Mogibacterium were enriched. In the lumen, predominant phylotypes related to metabolic disorders or metabolic exchange with the host, Erysipelotrichaceae, Prevotellaceae, and Coriobacteriaceae were increased in cancer patients. Coupled with previous reports, these results suggest that the intestinal microbiota is associated with CRC risk and that intestinal lumen microflora potentially influence CRC risk via cometabolism or metabolic exchange with the host. However, mucosa-associated microbiota potentially affects CRC risk primarily through direct interaction with the host.
format	article
author	Weiguang Chen Fanlong Liu Zongxin Ling Xiaojuan Tong Charlie Xiang
author_facet	Weiguang Chen Fanlong Liu Zongxin Ling Xiaojuan Tong Charlie Xiang
author_sort	Weiguang Chen
title	Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
title_short	Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
title_full	Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
title_fullStr	Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
title_full_unstemmed	Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
title_sort	human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b02c07f2e56046899e6d7c1063a93062
work_keys_str_mv	AT weiguangchen humanintestinallumenandmucosaassociatedmicrobiotainpatientswithcolorectalcancer AT fanlongliu humanintestinallumenandmucosaassociatedmicrobiotainpatientswithcolorectalcancer AT zongxinling humanintestinallumenandmucosaassociatedmicrobiotainpatientswithcolorectalcancer AT xiaojuantong humanintestinallumenandmucosaassociatedmicrobiotainpatientswithcolorectalcancer AT charliexiang humanintestinallumenandmucosaassociatedmicrobiotainpatientswithcolorectalcancer
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Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.

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