ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation

The bacterial antigen, lipopolysaccharide (LPS) and disruptions in calcium channels are independently known to influence oral cancer progression. Previously, we found that bacterial antigens, LPS and lipoteichoic acid (LTA) act as confounders during the action of capsaicin on Cal 27 oral cancer prol...

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Autores principales:	Rajdeep Chakraborty, Honghua Hu, Charbel Darido, Karen Vickery, Shoba Ranganathan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lipopolysaccharide lipoteichoic acid oral cancer ML218 HCl capsaicin proliferation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/b02dc11edf2f412eacdd208973f363d8
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spelling	oai:doaj.org-article:b02dc11edf2f412eacdd208973f363d82021-11-25T17:57:49ZML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation10.3390/ijms2222125591422-00671661-6596https://doaj.org/article/b02dc11edf2f412eacdd208973f363d82021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12559https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The bacterial antigen, lipopolysaccharide (LPS) and disruptions in calcium channels are independently known to influence oral cancer progression. Previously, we found that bacterial antigens, LPS and lipoteichoic acid (LTA) act as confounders during the action of capsaicin on Cal 27 oral cancer proliferation. As calcium channel drugs may affect oral cancer cell proliferation, we investigated the effect of ML218 HCl, a T-type voltage-gated calcium channel blocker, on the proliferation of Cal 27 oral cancer cells. We hypothesized that ML218 HCl could effectively reduce LPS-induced oral cancer cell proliferation. LPS and LTA antigens were added to Cal 27 oral cancer cells either prior to and/or concurrently with ML218 HCl treatment, and the efficacy of the treatment was evaluated by measuring Cal 27 proliferation, cell death and apoptosis. ML218 HCl inhibited oral cancer cell proliferation, increased apoptosis and cell death, but their efficacy was significantly reduced in the presence of bacterial antigens. ML218 HCl proved more effective than capsaicin in reducing bacterial antigen-induced Cal 27 oral cancer cell proliferation. Our results also suggest an interplay of proliferation factors during the bacterial antigens and calcium channel drug interaction in Cal 27. Bacterial antigen reduction of drug efficacy should be considered for developing newer pharmacological agents or testing the efficacy of the existing oral cancer chemotherapeutic agents. Finally, voltage gated calcium channel drugs should be considered for future oral cancer research.Rajdeep ChakrabortyHonghua HuCharbel DaridoKaren VickeryShoba RanganathanMDPI AGarticlelipopolysaccharidelipoteichoic acidoral cancerML218 HClcapsaicinproliferationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12559, p 12559 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipopolysaccharide lipoteichoic acid oral cancer ML218 HCl capsaicin proliferation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	lipopolysaccharide lipoteichoic acid oral cancer ML218 HCl capsaicin proliferation Biology (General) QH301-705.5 Chemistry QD1-999 Rajdeep Chakraborty Honghua Hu Charbel Darido Karen Vickery Shoba Ranganathan ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
description	The bacterial antigen, lipopolysaccharide (LPS) and disruptions in calcium channels are independently known to influence oral cancer progression. Previously, we found that bacterial antigens, LPS and lipoteichoic acid (LTA) act as confounders during the action of capsaicin on Cal 27 oral cancer proliferation. As calcium channel drugs may affect oral cancer cell proliferation, we investigated the effect of ML218 HCl, a T-type voltage-gated calcium channel blocker, on the proliferation of Cal 27 oral cancer cells. We hypothesized that ML218 HCl could effectively reduce LPS-induced oral cancer cell proliferation. LPS and LTA antigens were added to Cal 27 oral cancer cells either prior to and/or concurrently with ML218 HCl treatment, and the efficacy of the treatment was evaluated by measuring Cal 27 proliferation, cell death and apoptosis. ML218 HCl inhibited oral cancer cell proliferation, increased apoptosis and cell death, but their efficacy was significantly reduced in the presence of bacterial antigens. ML218 HCl proved more effective than capsaicin in reducing bacterial antigen-induced Cal 27 oral cancer cell proliferation. Our results also suggest an interplay of proliferation factors during the bacterial antigens and calcium channel drug interaction in Cal 27. Bacterial antigen reduction of drug efficacy should be considered for developing newer pharmacological agents or testing the efficacy of the existing oral cancer chemotherapeutic agents. Finally, voltage gated calcium channel drugs should be considered for future oral cancer research.
format	article
author	Rajdeep Chakraborty Honghua Hu Charbel Darido Karen Vickery Shoba Ranganathan
author_facet	Rajdeep Chakraborty Honghua Hu Charbel Darido Karen Vickery Shoba Ranganathan
author_sort	Rajdeep Chakraborty
title	ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
title_short	ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
title_full	ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
title_fullStr	ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
title_full_unstemmed	ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
title_sort	ml218 hcl is more efficient than capsaicin in inhibiting bacterial antigen-induced cal 27 oral cancer cell proliferation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b02dc11edf2f412eacdd208973f363d8
work_keys_str_mv	AT rajdeepchakraborty ml218hclismoreefficientthancapsaicinininhibitingbacterialantigeninducedcal27oralcancercellproliferation AT honghuahu ml218hclismoreefficientthancapsaicinininhibitingbacterialantigeninducedcal27oralcancercellproliferation AT charbeldarido ml218hclismoreefficientthancapsaicinininhibitingbacterialantigeninducedcal27oralcancercellproliferation AT karenvickery ml218hclismoreefficientthancapsaicinininhibitingbacterialantigeninducedcal27oralcancercellproliferation AT shobaranganathan ml218hclismoreefficientthancapsaicinininhibitingbacterialantigeninducedcal27oralcancercellproliferation
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ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation

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