Benthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights

Apart from its contribution to climate change, offshore oil and gas extraction is also a potential threat to the diversity and function of marine ecosystems. Routine monitoring of the environmental status of affected areas is therefore critical for effective management. While current morphology-base...

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Autores principales:	Anders Lanzén, Thomas G. Dahlgren, Andrea Bagi, Jon Thomassen Hestetun
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Environmental DNA Environmental monitoring Marine ecosystems Metabarcoding Benthos Environmental assessment Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/b0322fde0cc04616b40e4cdc5c3caf01
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spelling	oai:doaj.org-article:b0322fde0cc04616b40e4cdc5c3caf012021-12-01T04:58:42ZBenthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights1470-160X10.1016/j.ecolind.2021.108064https://doaj.org/article/b0322fde0cc04616b40e4cdc5c3caf012021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21007299https://doaj.org/toc/1470-160XApart from its contribution to climate change, offshore oil and gas extraction is also a potential threat to the diversity and function of marine ecosystems. Routine monitoring of the environmental status of affected areas is therefore critical for effective management. While current morphology-based monitoring is relatively time consuming, costly and prone to identification bias, environmental DNA metabarcoding offers an attractive alternative for assessing the impacts of oil drilling, extraction or spills. However, to be ready for routine monitoring, its performance needs to be demonstrated through agreement with assessments based on physicochemical measurements and current bioindicators. To this end, we applied metabarcoding to sequence the metazoan (COI) and total eukaryotic (18S) benthic components. We targeted a range of sites, with a gradient of low to high level of impact, located near active production installations and reference sites, in the North and Barents Seas. Alpha diversity and community structure of both datasets correlated strongly with a physicochemical pressure index (PI) based on total hydrocarbons (THC), PAH16, Ba and Cu. Calculations of the macroinvertebrate-based Norwegian Sensitivity Index (NSI) based on COI metabarcoding data agreed well with corresponding morpho-taxonomy values and with the PI. Further, we identified a set of bioindicator taxa from both metabarcoding datasets, to develop novel biotic indices and demonstrate their predictive performance using cross-validation. Finally, we compared co-occurrence networks from impacted vs. non-impacted sites, to improve the understanding of the ecological consequences of impacts. Our study demonstrates that metabarcoding can act as a meaningful and relatively accurate complement to the current morpho-taxonomic approach.Anders LanzénThomas G. DahlgrenAndrea BagiJon Thomassen HestetunElsevierarticleEnvironmental DNAEnvironmental monitoringMarine ecosystemsMetabarcodingBenthosEnvironmental assessmentEcologyQH540-549.5ENEcological Indicators, Vol 130, Iss , Pp 108064- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Environmental DNA Environmental monitoring Marine ecosystems Metabarcoding Benthos Environmental assessment Ecology QH540-549.5
spellingShingle	Environmental DNA Environmental monitoring Marine ecosystems Metabarcoding Benthos Environmental assessment Ecology QH540-549.5 Anders Lanzén Thomas G. Dahlgren Andrea Bagi Jon Thomassen Hestetun Benthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights
description	Apart from its contribution to climate change, offshore oil and gas extraction is also a potential threat to the diversity and function of marine ecosystems. Routine monitoring of the environmental status of affected areas is therefore critical for effective management. While current morphology-based monitoring is relatively time consuming, costly and prone to identification bias, environmental DNA metabarcoding offers an attractive alternative for assessing the impacts of oil drilling, extraction or spills. However, to be ready for routine monitoring, its performance needs to be demonstrated through agreement with assessments based on physicochemical measurements and current bioindicators. To this end, we applied metabarcoding to sequence the metazoan (COI) and total eukaryotic (18S) benthic components. We targeted a range of sites, with a gradient of low to high level of impact, located near active production installations and reference sites, in the North and Barents Seas. Alpha diversity and community structure of both datasets correlated strongly with a physicochemical pressure index (PI) based on total hydrocarbons (THC), PAH16, Ba and Cu. Calculations of the macroinvertebrate-based Norwegian Sensitivity Index (NSI) based on COI metabarcoding data agreed well with corresponding morpho-taxonomy values and with the PI. Further, we identified a set of bioindicator taxa from both metabarcoding datasets, to develop novel biotic indices and demonstrate their predictive performance using cross-validation. Finally, we compared co-occurrence networks from impacted vs. non-impacted sites, to improve the understanding of the ecological consequences of impacts. Our study demonstrates that metabarcoding can act as a meaningful and relatively accurate complement to the current morpho-taxonomic approach.
format	article
author	Anders Lanzén Thomas G. Dahlgren Andrea Bagi Jon Thomassen Hestetun
author_facet	Anders Lanzén Thomas G. Dahlgren Andrea Bagi Jon Thomassen Hestetun
author_sort	Anders Lanzén
title	Benthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights
title_short	Benthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights
title_full	Benthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights
title_fullStr	Benthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights
title_full_unstemmed	Benthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights
title_sort	benthic edna metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/b0322fde0cc04616b40e4cdc5c3caf01
work_keys_str_mv	AT anderslanzen benthicednametabarcodingprovidesaccurateassessmentsofimpactfromoilextractionandecologicalinsights AT thomasgdahlgren benthicednametabarcodingprovidesaccurateassessmentsofimpactfromoilextractionandecologicalinsights AT andreabagi benthicednametabarcodingprovidesaccurateassessmentsofimpactfromoilextractionandecologicalinsights AT jonthomassenhestetun benthicednametabarcodingprovidesaccurateassessmentsofimpactfromoilextractionandecologicalinsights
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Benthic eDNA metabarcoding provides accurate assessments of impact from oil extraction, and ecological insights

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